################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AlwI AocI ApaI AscI AseI AsnI Asp718I AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MroI MslI MspA1I MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 901 CGCG CG^CG AcsI 505 AAATTC [GA]^AATT[CT] AcyI 930 GACGCC G[GA]^CG[CT]C AfaI 315 GTAC GT^AC AhdI 450 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 67 GTGCAC G^TGCAC Aor51HI 365 AGCGCT AGC^GCT ApaLI 67 GTGCAC G^TGCAC ApoI 505 AAATTC [GA]^AATT[CT] Asp700I 174 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 450 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 98 TGGCCA TGG^CCA BglI 645 GCCCACACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 930 GACGCC G[GA]^CG[CT]C BsmBI 928 GAGACG CGTCTC(1/5) BsmI 164 GAATGC GAATGC(1/-1) BsrFI 407 ACCGGC [GA]^CCGG[CT] BssSI 292 CACGAG CACGAG(-5/-1) BstBI 161 TTCGAA TT^CGAA BstF5I 122 GGATG GGATG(2/0) BstUI 901 CGCG CG^CG Cfr10I 407 ACCGGC [GA]^CCGG[CT] DrdI 27 GACCGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 450 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 365 AGCGCT AGC^GCT FauI 119 GCGGG CCCGC(4/6) FokI 122 GGATG GGATG(9/13) HgaI 930 GACGC GACGC(5/10) Hin1I 930 GACGCC G[GA]^CG[CT]C HincII 474 GTTGAC GT[CT]^[GA]AC HindII 474 GTTGAC GT[CT]^[GA]AC MluNI 98 TGGCCA TGG^CCA MscI 98 TGGCCA TGG^CCA MseI 568 TTAA T^TAA MvnI 901 CGCG CG^CG NruI 900 TCGCGA TCG^CGA NspV 161 TTCGAA TT^CGAA PflMI 261 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 489 CTGCAG CTGCA^G RsaI 315 GTAC GT^AC SfcI 489 CTGCAG C^T[GA][CT]AG SfuI 161 TTCGAA TT^CGAA TaqI 162 TCGA T^CGA Tru9I 568 TTAA T^TAA Tsp509I 506 AATT ^AATT TthHB8I 162 TCGA T^CGA Van91I 261 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 174 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AlwNI 262 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 760 CAGGTCCTG AvaI 751 CCCGAG C^[CT]CG[GA]G 910 CCCGAG BanI 416 GGCACC G^G[CT][GA]CC 943 GGCACC BlpI 87 GCTGAGC GC^T[ACGT]AGC 246 GCTCAGC Bpu1102I 87 GCTGAGC GC^T[ACGT]AGC 246 GCTCAGC BsoBI 751 CCCGAG C^[CT]CG[GA]G 910 CCCGAG BspMI 109 ACCTGC ACCTGC(4/8) 759 GCAGGT BstXI 866 CCAGGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 947 CCAATGAGATGG CelII 87 GCTGAGC GC^T[ACGT]AGC 246 GCTCAGC DpnII 144 GATC ^GATC 517 GATC DraII 761 AGGTCCT [GA]G^G[ACGT]CC[CT] 868 AGGTCCC DsaI 29 CCGTGG C^C[GA][CT]GG 938 CCGTGG EaeI 98 TGGCCA [CT]^GGCC[GA] 350 CGGCCA EcoO109I 761 AGGTCCT [GA]G^G[ACGT]CC[CT] 868 AGGTCCC HapII 408 CCGG C^CGG 843 CCGG HinfI 34 GAGTC G^A[ACGT]TC 582 GACTC HpaII 408 CCGG C^CGG 843 CCGG MaeIII 404 GTCAC ^GT[ACGT]AC 780 GTCAC MboI 144 GATC ^GATC 517 GATC MspI 408 CCGG C^CGG 843 CCGG NdeII 144 GATC ^GATC 517 GATC PleI 582 GACTC GAGTC(4/5) 34 GAGTC PpuMI 761 AGGTCCT [GA]G^G[AT]CC[CT] 868 AGGTCCC SacI 90 GAGCTC GAGCT^C 621 GAGCTC Sau3AI 144 GATC ^GATC 517 GATC SexAI 80 ACCTGGT A^CC[AT]GGT 865 ACCAGGT Tsp45I 404 GTCAC ^GT[GC]AC 780 GTCAC XcmI 242 CCACGCTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 863 CCACCAGGTCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AluI AspHI AspI AvaII BanII BbvI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI EarI EcoRII Fnu4HI HaeII HaeIII HhaI HinP1I HphI ItaI Ksp632I MboII MnlI MvaI MwoI NlaIII NlaIV PflFI Sau96I ScrFI SfaNI TseI TspRI Tth111I