################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI NaeI NarI NciI NcoI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI TaqI TfiI Tru9I Tsp45I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 17 CGCG CG^CG AcsI 604 AAATTC [GA]^AATT[CT] AfaI 228 GTAC GT^AC Aor51HI 98 AGCGCT AGC^GCT ApoI 604 AAATTC [GA]^AATT[CT] Asp700I 342 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 437 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 342 GAAGAC GAAGAC(2/6) BlpI 490 GCTGAGC GC^T[ACGT]AGC Bpu1102I 490 GCTGAGC GC^T[ACGT]AGC BpuAI 342 GAAGAC GAAGAC(2/6) BsaI 358 GAGACC GGTCTC(1/5) BseRI 263 CTCCTC GAGGAG(10/8) BsgI 255 CTGCAC GTGCAG(16/14) BsiEI 436 CGACCG CG[GA][CT]^CG BsmAI 358 GAGAC GTCTC(1/5) Bsp1407I 227 TGTACA T^GTACA BsrGI 227 TGTACA T^GTACA BstF5I 111 CATCC GGATG(2/0) BstUI 17 CGCG CG^CG CelII 490 GCTGAGC GC^T[ACGT]AGC CfoI 99 GCGC GCG^C Eco47III 98 AGCGCT AGC^GCT FauI 125 GCGGG CCCGC(4/6) FokI 111 CATCC GGATG(9/13) HaeII 98 AGCGCT [GA]GCGC^[CT] HhaI 99 GCGC GCG^C HinP1I 99 GCGC G^CGC MaeII 518 ACGT A^CGT MvnI 17 CGCG CG^CG NdeI 337 CATATG CA^TATG NspI 129 GCATGC [GA]CATG^[CT] PflFI 437 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC RsaI 228 GTAC GT^AC SacI 54 GAGCTC GAGCT^C SfaNI 110 GCATC GCATC(5/9) SphI 129 GCATGC GCATG^C SspBI 227 TGTACA T^GTACA Tth111I 437 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 342 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AspHI 54 GAGCTC G[AT]GC[AT]^C 136 GAGCAC AvaII 8 GGACC G^G[AT]CC 187 GGACC BsiHKAI 54 GAGCTC G[AT]GC[AT]^C 136 GAGCAC EcoT14I 298 CCAAGG C^C[AT][AT]GG 315 CCAAGG HinfI 483 GAGTC G^A[ACGT]TC 503 GAGTC PflMI 166 CCATGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 298 CCAAGGACTGG PleI 483 GAGTC GAGTC(4/5) 503 GAGTC StyI 298 CCAAGG C^C[AT][AT]GG 315 CCAAGG Tsp509I 427 AATT ^AATT 605 AATT TspRI 351 CACTG CA[GC]TG[ACGT][ACGT]^ 593 CAGTG Van91I 166 CCATGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 298 CCAAGGACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AciI AluI BanI BanII BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII HphI ItaI MboII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TseI