Restriction map for pF1KB7394
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AvaI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BpmI        BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiWI       
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HapII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MspI        MunI        NaeI        
NarI        NciI        NcoI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspI        StuI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      TthHB8I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccII          17  CGCG                 CG^CG
AcsI          604  AAATTC               [GA]^AATT[CT]
AfaI          228  GTAC                 GT^AC
Aor51HI        98  AGCGCT               AGC^GCT
ApoI          604  AAATTC               [GA]^AATT[CT]
Asp700I       342  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          437  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI          342  GAAGAC               GAAGAC(2/6)
BlpI          490  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      490  GCTGAGC              GC^T[ACGT]AGC
BpuAI         342  GAAGAC               GAAGAC(2/6)
BsaI          358  GAGACC               GGTCTC(1/5)
BseRI         263  CTCCTC               GAGGAG(10/8)
BsgI          255  CTGCAC               GTGCAG(16/14)
BsiEI         436  CGACCG               CG[GA][CT]^CG
BsmAI         358  GAGAC                GTCTC(1/5)
Bsp1407I      227  TGTACA               T^GTACA
BsrGI         227  TGTACA               T^GTACA
BstF5I        111  CATCC                GGATG(2/0)
BstUI          17  CGCG                 CG^CG
CelII         490  GCTGAGC              GC^T[ACGT]AGC
CfoI           99  GCGC                 GCG^C
Eco47III       98  AGCGCT               AGC^GCT
FauI          125  GCGGG                CCCGC(4/6)
FokI          111  CATCC                GGATG(9/13)
HaeII          98  AGCGCT               [GA]GCGC^[CT]
HhaI           99  GCGC                 GCG^C
HinP1I         99  GCGC                 G^CGC
MaeII         518  ACGT                 A^CGT
MvnI           17  CGCG                 CG^CG
NdeI          337  CATATG               CA^TATG
NspI          129  GCATGC               [GA]CATG^[CT]
PflFI         437  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RsaI          228  GTAC                 GT^AC
SacI           54  GAGCTC               GAGCT^C
SfaNI         110  GCATC                GCATC(5/9)
SphI          129  GCATGC               GCATG^C
SspBI         227  TGTACA               T^GTACA
Tth111I       437  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          342  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AspHI          54  GAGCTC               G[AT]GC[AT]^C
              136  GAGCAC              
AvaII           8  GGACC                G^G[AT]CC
              187  GGACC               
BsiHKAI        54  GAGCTC               G[AT]GC[AT]^C
              136  GAGCAC              
EcoT14I       298  CCAAGG               C^C[AT][AT]GG
              315  CCAAGG              
HinfI         483  GAGTC                G^A[ACGT]TC
              503  GAGTC               
PflMI         166  CCATGCTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              298  CCAAGGACTGG         
PleI          483  GAGTC                GAGTC(4/5)
              503  GAGTC               
StyI          298  CCAAGG               C^C[AT][AT]GG
              315  CCAAGG              
Tsp509I       427  AATT                 ^AATT
              605  AATT                
TspRI         351  CACTG                CA[GC]TG[ACGT][ACGT]^
              593  CAGTG               
Van91I        166  CCATGCTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              298  CCAAGGACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AciI        AluI        BanI        BanII       BbvI        
BmyI        BsaJI       BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstNI       Cac8I       
Cfr13I      DdeI        EcoRII      Fnu4HI      HaeIII      
HphI        ItaI        MboII       MnlI        MvaI        
MwoI        NlaIII      NlaIV       Sau96I      ScrFI       
TseI        
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