################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI Alw44I AlwNI ApaI ApaLI ApoI AscI Asp700I AspHI AspI AvaI AviII BalI BanII BbeI BbrPI BfrI BglII BmyI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccII 10 CGCG CG^CG Aor51HI 387 AGCGCT AGC^GCT BamHI 339 GGATCC G^GATCC BcgI 686 CGAGGCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 615 TGATCA T^GATCA BglI 905 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 234 GCTCAGC GC^T[ACGT]AGC Bpu1102I 234 GCTCAGC GC^T[ACGT]AGC BsaI 590 GGTCTC GGTCTC(1/5) BsgI 283 GTGCAG GTGCAG(16/14) BsmAI 591 GTCTC GTCTC(1/5) BspMI 264 ACCTGC ACCTGC(4/8) BsrBI 188 CCGCTC CCGCTC(-3/-3) BstUI 10 CGCG CG^CG BstYI 339 GGATCC [GA]^GATC[CT] CelII 234 GCTCAGC GC^T[ACGT]AGC DraII 663 AGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 317 CCATGG C^C[GA][CT]GG EarI 915 CTCTTC CTCTTC(1/4) Eco47III 387 AGCGCT AGC^GCT EcoO109I 663 AGGCCCT [GA]G^G[ACGT]CC[CT] FauI 187 CCCGC CCCGC(4/6) FbaI 615 TGATCA T^GATCA HaeII 387 AGCGCT [GA]GCGC^[CT] Ksp632I 915 CTCTTC CTCTTC(1/4) MflI 339 GGATCC [GA]^GATC[CT] MvnI 10 CGCG CG^CG NcoI 317 CCATGG C^CATGG PflMI 111 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 914 GCTCTTC GCTCTTC(1/4) SbfI 265 CCTGCAGG CCTGCA^GG Sse8387I 265 CCTGCAGG CCTGCA^GG Tsp509I 544 AATT ^AATT Van91I 111 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 339 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 628 GGTACC G^GTACC 739 GGTACC AfaI 629 GTAC GT^AC 740 GTAC AhdI 196 GACCCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 514 GACTCGGAGTC AlwI 340 GATCC GGATC(4/5) 339 GGATC AocI 334 CCTGAGG CC^T[ACGT]AGG 703 CCTGAGG Asp718I 628 GGTACC G^GTACC 739 GGTACC AspEI 196 GACCCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 514 GACTCGGAGTC AvrII 199 CCTAGG C^CTAGG 958 CCTAGG BanI 628 GGTACC G^G[CT][GA]CC 739 GGTACC BbsI 53 GTCTTC GAAGAC(2/6) 462 GTCTTC BbvCI 133 CCTCAGC CCTCAGC(-5/-2) 367 GCTGAGG BfaI 200 CTAG C^TAG 959 CTAG BlnI 199 CCTAGG C^CTAGG 958 CCTAGG BpmI 414 CTCCAG CTGGAG(16/14) 893 CTGGAG BpuAI 53 GTCTTC GAAGAC(2/6) 462 GTCTTC BsmFI 121 GGGAC GGGAC(10/14) 670 GGGAC Bsu36I 334 CCTGAGG CC^T[ACGT]AGG 703 CCTGAGG CfoI 26 GCGC GCG^C 388 GCGC DpnII 340 GATC ^GATC 616 GATC Eam1105I 196 GACCCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 514 GACTCGGAGTC Eco81I 334 CCTGAGG CC^T[ACGT]AGG 703 CCTGAGG EcoNI 265 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 504 CCTCCACCAGG HhaI 26 GCGC GCG^C 388 GCGC HinP1I 26 GCGC G^CGC 388 GCGC HphI 487 TCACC GGTGA(8/7) 618 TCACC KpnI 628 GGTACC GGTAC^C 739 GGTACC MaeI 200 CTAG C^TAG 959 CTAG MboI 340 GATC ^GATC 616 GATC NdeII 340 GATC ^GATC 616 GATC NlaIII 318 CATG CATG^ 693 CATG PstI 266 CTGCAG CTGCA^G 278 CTGCAG RsaI 629 GTAC GT^AC 740 GTAC Sau3AI 340 GATC ^GATC 616 GATC SfcI 266 CTGCAG C^T[GA][CT]AG 278 CTGCAG StuI 66 AGGCCT AGG^CCT 480 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AluI AseI AsnI AvaII BbvI BciVI BcnI BsaJI BseRI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NlaIV PleI PshBI PvuII Sau96I ScrFI SfaNI SspI StyI TfiI Tru9I TseI TspRI XcmI