##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI Alw44I
AlwNI ApaI ApaLI ApoI AscI
Asp700I AspHI AspI AvaI AviII
BalI BanII BbeI BbrPI BfrI
BglII BmyI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SwaI TaqI
Tsp45I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 10 CGCG CG^CG
Aor51HI 387 AGCGCT AGC^GCT
BamHI 339 GGATCC G^GATCC
BcgI 686 CGAGGCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 615 TGATCA T^GATCA
BglI 905 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 234 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 234 GCTCAGC GC^T[ACGT]AGC
BsaI 590 GGTCTC GGTCTC(1/5)
BsgI 283 GTGCAG GTGCAG(16/14)
BsmAI 591 GTCTC GTCTC(1/5)
BspMI 264 ACCTGC ACCTGC(4/8)
BsrBI 188 CCGCTC CCGCTC(-3/-3)
BstUI 10 CGCG CG^CG
BstYI 339 GGATCC [GA]^GATC[CT]
CelII 234 GCTCAGC GC^T[ACGT]AGC
DraII 663 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 317 CCATGG C^C[GA][CT]GG
EarI 915 CTCTTC CTCTTC(1/4)
Eco47III 387 AGCGCT AGC^GCT
EcoO109I 663 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 187 CCCGC CCCGC(4/6)
FbaI 615 TGATCA T^GATCA
HaeII 387 AGCGCT [GA]GCGC^[CT]
Ksp632I 915 CTCTTC CTCTTC(1/4)
MflI 339 GGATCC [GA]^GATC[CT]
MvnI 10 CGCG CG^CG
NcoI 317 CCATGG C^CATGG
PflMI 111 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 914 GCTCTTC GCTCTTC(1/4)
SbfI 265 CCTGCAGG CCTGCA^GG
Sse8387I 265 CCTGCAGG CCTGCA^GG
Tsp509I 544 AATT ^AATT
Van91I 111 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 339 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 628 GGTACC G^GTACC
739 GGTACC
AfaI 629 GTAC GT^AC
740 GTAC
AhdI 196 GACCCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
514 GACTCGGAGTC
AlwI 340 GATCC GGATC(4/5)
339 GGATC
AocI 334 CCTGAGG CC^T[ACGT]AGG
703 CCTGAGG
Asp718I 628 GGTACC G^GTACC
739 GGTACC
AspEI 196 GACCCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
514 GACTCGGAGTC
AvrII 199 CCTAGG C^CTAGG
958 CCTAGG
BanI 628 GGTACC G^G[CT][GA]CC
739 GGTACC
BbsI 53 GTCTTC GAAGAC(2/6)
462 GTCTTC
BbvCI 133 CCTCAGC CCTCAGC(-5/-2)
367 GCTGAGG
BfaI 200 CTAG C^TAG
959 CTAG
BlnI 199 CCTAGG C^CTAGG
958 CCTAGG
BpmI 414 CTCCAG CTGGAG(16/14)
893 CTGGAG
BpuAI 53 GTCTTC GAAGAC(2/6)
462 GTCTTC
BsmFI 121 GGGAC GGGAC(10/14)
670 GGGAC
Bsu36I 334 CCTGAGG CC^T[ACGT]AGG
703 CCTGAGG
CfoI 26 GCGC GCG^C
388 GCGC
DpnII 340 GATC ^GATC
616 GATC
Eam1105I 196 GACCCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
514 GACTCGGAGTC
Eco81I 334 CCTGAGG CC^T[ACGT]AGG
703 CCTGAGG
EcoNI 265 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
504 CCTCCACCAGG
HhaI 26 GCGC GCG^C
388 GCGC
HinP1I 26 GCGC G^CGC
388 GCGC
HphI 487 TCACC GGTGA(8/7)
618 TCACC
KpnI 628 GGTACC GGTAC^C
739 GGTACC
MaeI 200 CTAG C^TAG
959 CTAG
MboI 340 GATC ^GATC
616 GATC
NdeII 340 GATC ^GATC
616 GATC
NlaIII 318 CATG CATG^
693 CATG
PstI 266 CTGCAG CTGCA^G
278 CTGCAG
RsaI 629 GTAC GT^AC
740 GTAC
Sau3AI 340 GATC ^GATC
616 GATC
SfcI 266 CTGCAG C^T[GA][CT]AG
278 CTGCAG
StuI 66 AGGCCT AGG^CCT
480 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AseI AsnI AvaII
BbvI BciVI BcnI BsaJI BseRI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NciI NlaIV PleI PshBI PvuII
Sau96I ScrFI SfaNI SspI StyI
TfiI Tru9I TseI TspRI XcmI