Restriction map for
pF1KB7399
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BseRI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DraII DraIII DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeIII MamI MfeI MluI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StyI
SwaI Tsp45I Tsp509I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 179 TCCGGA T^CCGGA
AfaI 432 GTAC GT^AC
AocI 165 CCTGAGG CC^T[ACGT]AGG
AvaII 389 GGACC G^G[AT]CC
BalI 89 TGGCCA TGG^CCA
BanII 501 GAGCCC G[GA]GC[CT]^C
BmyI 501 GAGCCC G[AGT]GC[ACT]^C
BpmI 370 CTCCAG CTGGAG(16/14)
BsaWI 179 TCCGGA [AT]^CCGG[AT]
BseAI 179 TCCGGA T^CCGGA
BsgI 235 GTGCAG GTGCAG(16/14)
BsmBI 330 CGTCTC CGTCTC(1/5)
BsmFI 388 GGGAC GGGAC(10/14)
Bsp1286I 501 GAGCCC G[AGT]GC[ACT]^C
BspEI 179 TCCGGA T^CCGGA
BspMI 421 ACCTGC ACCTGC(4/8)
BstBI 223 TTCGAA TT^CGAA
BstYI 474 AGATCC [GA]^GATC[CT]
Bsu36I 165 CCTGAGG CC^T[ACGT]AGG
CfoI 409 GCGC GCG^C
DrdI 163 GACCTGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 165 CCTGAGG CC^T[ACGT]AGG
FauI 504 CCCGC CCCGC(4/6)
HaeII 408 GGCGCT [GA]GCGC^[CT]
HapII 180 CCGG C^CGG
HhaI 409 GCGC GCG^C
HinP1I 409 GCGC G^CGC
HinfI 141 GAATC G^A[ACGT]TC
HpaII 180 CCGG C^CGG
HphI 359 TCACC GGTGA(8/7)
MaeII 86 ACGT A^CGT
MflI 474 AGATCC [GA]^GATC[CT]
MluNI 89 TGGCCA TGG^CCA
MroI 179 TCCGGA T^CCGGA
MscI 89 TGGCCA TGG^CCA
MseI 149 TTAA T^TAA
MslI 63 CACCGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 180 CCGG C^CGG
MwoI 513 GCCTCCCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspV 223 TTCGAA TT^CGAA
RsaI 432 GTAC GT^AC
SfcI 345 CTACAG C^T[GA][CT]AG
SfuI 223 TTCGAA TT^CGAA
StuI 511 AGGCCT AGG^CCT
TfiI 141 GAATC G^A[AT]TC
Tru9I 149 TTAA T^TAA
TspRI 323 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 474 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AciI 505 CCGC CCGC(-3/-1)
522 GCGG
AlwI 55 GATCC GGATC(4/5)
475 GATCC
BbsI 273 GAAGAC GAAGAC(2/6)
172 GTCTTC
BbvI 40 GCAGC GCAGC(8/12)
206 GCAGC
BlpI 15 GCTGAGC GC^T[ACGT]AGC
27 GCTGAGC
Bpu1102I 15 GCTGAGC GC^T[ACGT]AGC
27 GCTGAGC
BpuAI 273 GAAGAC GAAGAC(2/6)
172 GTCTTC
BsaI 161 GAGACC GGTCTC(1/5)
394 GAGACC
BsiYI 399 CCACACGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
518 CCATGCGGTGG
BslI 399 CCACACGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
518 CCATGCGGTGG
Cac8I 405 GCTGGC GC[ACGT]^[ACGT]GC
503 GCCCGC
CelII 15 GCTGAGC GC^T[ACGT]AGC
27 GCTGAGC
Cfr13I 312 GGGCC G^G[ACGT]CC
389 GGACC
DpnII 55 GATC ^GATC
475 GATC
EaeI 89 TGGCCA [CT]^GGCC[GA]
326 TGGCCG
Fnu4HI 40 GCAGC GC^[ACGT]GC
206 GCAGC
ItaI 40 GCAGC GC^[ACGT]GC
206 GCAGC
MboI 55 GATC ^GATC
475 GATC
NdeII 55 GATC ^GATC
475 GATC
NlaIV 388 GGGACC GG[ACGT]^[ACGT]CC
500 GGAGCC
PflMI 399 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
518 CCATGCGGTGG
Sau3AI 55 GATC ^GATC
475 GATC
Sau96I 312 GGGCC G^G[ACGT]CC
389 GGACC
SspI 184 AATATT AAT^ATT
213 AATATT
TseI 40 GCAGC G^C[AT]GC
206 GCAGC
Van91I 399 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
518 CCATGCGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
BsaJI BsmAI BssKI BstNI DdeI
EcoRII HaeIII MboII MnlI MvaI
NlaIII ScrFI TaqI TthHB8I