Restriction map for
pF1KB7401
##################################
# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HgaI Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
MaeI MaeII MaeIII MamI MflI
MluI MluNI MroI MscI MseI
NarI NciI NcoI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tru9I Tsp45I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AfaI 433 GTAC GT^AC
Aor51HI 173 AGCGCT AGC^GCT
ApaI 195 GGGCCC GGGCC^C
AspHI 451 GAGCTC G[AT]GC[AT]^C
AvaII 75 GGTCC G^G[AT]CC
BanI 427 GGCACC G^G[CT][GA]CC
BcgI 501 CGATCAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 141 GCTGAGC GC^T[ACGT]AGC
BpmI 454 CTCCAG CTGGAG(16/14)
Bpu1102I 141 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 451 GAGCTC G[AT]GC[AT]^C
BsmAI 240 GTCTC GTCTC(1/5)
BsrFI 201 GCCGGC [GA]^CCGG[CT]
BssSI 298 CACGAG CACGAG(-5/-1)
CelII 141 GCTGAGC GC^T[ACGT]AGC
Cfr10I 201 GCCGGC [GA]^CCGG[CT]
DpnII 502 GATC ^GATC
DraII 194 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 396 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 105 CCGCGG C^C[GA][CT]GG
Eco47III 173 AGCGCT AGC^GCT
EcoNI 285 CCTATGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 194 GGGGCCC [GA]G^G[ACGT]CC[CT]
HapII 202 CCGG C^CGG
HpaII 202 CCGG C^CGG
KspI 105 CCGCGG CCGC^GG
MboI 502 GATC ^GATC
MfeI 512 CAATTG C^AATTG
MspI 202 CCGG C^CGG
MunI 512 CAATTG C^AATTG
NaeI 201 GCCGGC GCC^GGC
NdeII 502 GATC ^GATC
NgoMIV 201 GCCGGC G^CCGGC
NotI 107 GCGGCCGC GC^GGCCGC
NspI 134 GCATGT [GA]CATG^[CT]
PvuII 441 CAGCTG CAG^CTG
RsaI 433 GTAC GT^AC
SacI 451 GAGCTC GAGCT^C
SacII 105 CCGCGG CCGC^GG
Sau3AI 502 GATC ^GATC
TaqI 464 TCGA T^CGA
TspRI 28 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 464 TCGA T^CGA
XcmI 377 CCATCGTGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BbsI 352 GTCTTC GAAGAC(2/6)
361 GTCTTC
BbvI 113 GCTGC GCAGC(8/12)
246 GCTGC
BpuAI 352 GTCTTC GAAGAC(2/6)
361 GTCTTC
BsgI 160 GTGCAG GTGCAG(16/14)
184 GTGCAG
BsiEI 108 CGGCCG CG[GA][CT]^CG
203 CGGCCG
BsmFI 294 GGGAC GGGAC(10/14)
76 GTCCC
BsrDI 366 CATTGC GCAATG(2/0)
553 GCAATG
DdeI 26 CTCAG C^T[ACGT]AG
142 CTGAG
EagI 108 CGGCCG C^GGCCG
203 CGGCCG
EclXI 108 CGGCCG C^GGCCG
203 CGGCCG
Eco52I 108 CGGCCG C^GGCCG
203 CGGCCG
FauI 78 CCCGC CCCGC(4/6)
198 CCCGC
MslI 375 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
439 CACAGCTGTG
NlaIII 135 CATG CATG^
399 CATG
TseI 113 GCTGC G^C[AT]GC
246 GCTGC
Tsp509I 13 AATT ^AATT
513 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AluI BanII BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
EaeI EarI EcoRII Fnu4HI HaeII
HaeIII HhaI HinP1I HphI ItaI
Ksp632I MboII MnlI MspA1I MvaI
MvnI MwoI NlaIV SapI Sau96I
ScrFI