Restriction map for
pF1KB7403
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI CelII ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
HphI KasI KpnI KspI MaeII
MluI MroI NarI NcoI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccII 521 CGCG CG^CG
AlwNI 957 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 472 CCTAAGG CC^T[ACGT]AGG
Asp700I 202 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 665 TGGCCA TGG^CCA
BamHI 577 GGATCC G^GATCC
BanI 853 GGCACC G^G[CT][GA]CC
BbsI 821 GTCTTC GAAGAC(2/6)
BcnI 1449 CCCGG CC^[GC]GG
BmyI 455 GGGCAC G[AGT]GC[ACT]^C
BpuAI 821 GTCTTC GAAGAC(2/6)
BsaBI 578 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 305 GGGAC GGGAC(10/14)
Bsp1286I 455 GGGCAC G[AGT]GC[ACT]^C
BspHI 234 TCATGA T^CATGA
BsrDI 1148 GCAATG GCAATG(2/0)
BsrFI 485 GCCGGC [GA]^CCGG[CT]
Bst1107I 808 GTATAC GTA^TAC
BstUI 521 CGCG CG^CG
BstXI 566 CCATTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 577 GGATCC [GA]^GATC[CT]
BstZ17I 808 GTATAC GTA^TAC
Bsu36I 472 CCTAAGG CC^T[ACGT]AGG
CfoI 520 GCGC GCG^C
Cfr10I 485 GCCGGC [GA]^CCGG[CT]
DpnII 578 GATC ^GATC
DraI 1155 TTTAAA TTT^AAA
EaeI 665 TGGCCA [CT]^GGCC[GA]
EarI 1261 GAAGAG CTCTTC(1/4)
Eco81I 472 CCTAAGG CC^T[ACGT]AGG
EcoRI 1168 GAATTC G^AATTC
FauI 545 CCCGC CCCGC(4/6)
HhaI 520 GCGC GCG^C
HinP1I 520 GCGC G^CGC
HindIII 660 AAGCTT A^AGCTT
Ksp632I 1261 GAAGAG CTCTTC(1/4)
MamI 578 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 578 GATC ^GATC
MfeI 1319 CAATTG C^AATTG
MflI 577 GGATCC [GA]^GATC[CT]
MluNI 665 TGGCCA TGG^CCA
MscI 665 TGGCCA TGG^CCA
MspA1I 830 CAGCTG C[AC]G^C[GT]G
MunI 1319 CAATTG C^AATTG
MvnI 521 CGCG CG^CG
NaeI 485 GCCGGC GCC^GGC
NciI 1449 CCCGG CC^[GC]GG
NdeI 713 CATATG CA^TATG
NdeII 578 GATC ^GATC
NgoMIV 485 GCCGGC G^CCGGC
PflMI 1404 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 620 GACTC GAGTC(4/5)
PvuII 830 CAGCTG CAG^CTG
RcaI 234 TCATGA T^CATGA
Sau3AI 578 GATC ^GATC
SexAI 889 ACCTGGT A^CC[AT]GGT
TaqI 141 TCGA T^CGA
TthHB8I 141 TCGA T^CGA
Van91I 1404 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 577 GGATCC [GA]^GATC[CT]
XmnI 202 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 808 GTATAC GT^[AC][GT]AC
1185 GTCTAC
AciI 546 CCGC CCGC(-3/-1)
155 GCGG
AlwI 578 GATCC GGATC(4/5)
577 GGATC
BfaI 719 CTAG C^TAG
805 CTAG
BpmI 633 CTGGAG CTGGAG(16/14)
1161 CTGGAG
BstNI 890 CCTGG CC^[AT]GG
1119 CCTGG
DraII 305 GGGACCT [GA]G^G[ACGT]CC[CT]
468 AGGACCT
EcoO109I 305 GGGACCT [GA]G^G[ACGT]CC[CT]
468 AGGACCT
EcoRII 890 CCTGG ^CC[AT]GG
1119 CCTGG
EcoT14I 877 CCTTGG C^C[AT][AT]GG
1101 CCTTGG
HaeIII 641 GGCC GG^CC
666 GGCC
HapII 486 CCGG C^CGG
1450 CCGG
HpaII 486 CCGG C^CGG
1450 CCGG
MaeI 719 CTAG C^TAG
805 CTAG
MspI 486 CCGG C^CGG
1450 CCGG
MvaI 890 CCTGG CC^[AT]GG
1119 CCTGG
MwoI 26 GCCCTAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1091 GCATAATGAGC
PpuMI 305 GGGACCT [GA]G^G[AT]CC[CT]
468 AGGACCT
ScaI 1216 AGTACT AGT^ACT
1265 AGTACT
StyI 877 CCTTGG C^C[AT][AT]GG
1101 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbvI BsaJI BsiYI BslI BsmAI
BspMI BsrI BssKI BstF5I Cac8I
Cfr13I DdeI Fnu4HI FokI HinfI
ItaI MaeIII MboII MnlI MseI
MslI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI SfcI SspI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI