Restriction map for
pF1KB7407
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaWI BseAI BseRI BsgI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BspMI BsrDI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI DpnII DraI
DraII DrdI Eam1105I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HindIII HinfI
HpaI KpnI MaeI MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NcoI NdeI
NdeII NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI SacI
SalI Sau3AI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TfiI Tru9I Tsp45I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 115 GTCTAC GT^[AC][GT]AC
AluI 392 AGCT AG^CT
Aor51HI 329 AGCGCT AGC^GCT
BanII 544 GAGCCC G[GA]GC[CT]^C
BbvCI 600 CCTCAGC CCTCAGC(-5/-2)
BsaI 523 GAGACC GGTCTC(1/5)
BsmAI 523 GAGAC GTCTC(1/5)
BsmFI 158 GGGAC GGGAC(10/14)
Bsp1407I 272 TGTACA T^GTACA
BsrBI 367 CCGCTC CCGCTC(-3/-3)
BsrGI 272 TGTACA T^GTACA
BsrI 119 ACTGG ACTGG(1/-1)
BstAPI 261 GCACTACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 609 CATCC GGATG(2/0)
BstXI 467 CCAATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 101 CGGACCG CG^G[AT]CCG
DraIII 262 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 350 CCGCGG C^C[GA][CT]GG
Eco47III 329 AGCGCT AGC^GCT
EcoNI 528 CCTGTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 609 CATCC GGATG(9/13)
HgaI 82 GACGC GACGC(5/10)
HincII 280 GTCAAC GT[CT]^[GA]AC
HindII 280 GTCAAC GT[CT]^[GA]AC
KspI 350 CCGCGG CCGC^GG
MaeIII 45 GTTAC ^GT[ACGT]AC
MslI 609 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 243 GCCGGC GCC^GGC
NgoMIV 243 GCCGGC G^CCGGC
RsrII 101 CGGACCG CG^G[AT]CCG
SacII 350 CCGCGG CCGC^GG
SapI 393 GCTCTTC GCTCTTC(1/4)
SfaNI 191 GCATC GCATC(5/9)
SmlI 370 CTCAAG C^T[CT][GA]AG
SspBI 272 TGTACA T^GTACA
TaqI 419 TCGA T^CGA
TthHB8I 419 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspHI 259 GAGCAC G[AT]GC[AT]^C
616 GTGCTC
AvaI 358 CCCGGG C^[CT]CG[GA]G
579 CCCGGG
BsaAI 265 TACGTG [CT]AC^GT[GA]
505 TACGTG
BsiEI 245 CGGCCG CG[GA][CT]^CG
511 CGGCCG
BsiHKAI 259 GAGCAC G[AT]GC[AT]^C
616 GTGCTC
BsoBI 358 CCCGGG C^[CT]CG[GA]G
579 CCCGGG
BssHII 70 GCGCGC G^CGCGC
72 GCGCGC
BssSI 436 CACGAG CACGAG(-5/-1)
567 CTCGTG
DdeI 542 CTGAG C^T[ACGT]AG
601 CTCAG
EagI 245 CGGCCG C^GGCCG
511 CGGCCG
EarI 394 CTCTTC CTCTTC(1/4)
595 CTCTTC
EclXI 245 CGGCCG C^GGCCG
511 CGGCCG
Eco52I 245 CGGCCG C^GGCCG
511 CGGCCG
HphI 186 GGTGA GGTGA(8/7)
554 TCACC
Ksp632I 394 CTCTTC CTCTTC(1/4)
595 CTCTTC
MaeII 266 ACGT A^CGT
506 ACGT
NlaIII 255 CATG CATG^
276 CATG
SmaI 358 CCCGGG CCC^GGG
579 CCCGGG
XmaCI 358 CCCGGG C^CCGGG
579 CCCGGG
XmaI 358 CCCGGG C^CCGGG
579 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AcyI AfaI AvaII
BanI BbeI BbvI BcnI BglI
BmyI BsaHI BsaJI BsiYI BslI
Bsp1286I BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I EaeI
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
ItaI KasI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIV RsaI Sau96I ScrFI
SfoI TseI