################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AocI Aor51HI ApoI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BcgI BciVI BfaI BfrI BglII BlnI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI HincII HindII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 236 GTCTAC GT^[AC][GT]AC AcyI 696 GGCGCC G[GA]^CG[CT]C AhdI 793 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 551 GTGCAC G^TGCAC ApaI 941 GGGCCC GGGCC^C ApaLI 551 GTGCAC G^TGCAC Asp700I 115 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 793 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 127 TGGCCA TGG^CCA BbeI 696 GGCGCC GGCGC^C BbrPI 496 CACGTG CAC^GTG BbvCI 908 CCTCAGC CCTCAGC(-5/-2) BclI 467 TGATCA T^GATCA BglI 620 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 496 CACGTG [CT]AC^GT[GA] BsaHI 696 GGCGCC G[GA]^CG[CT]C BseRI 315 GAGGAG GAGGAG(10/8) BsmI 135 GAATGC GAATGC(1/-1) Bsp1407I 363 TGTACA T^GTACA BspMI 653 ACCTGC ACCTGC(4/8) BsrGI 363 TGTACA T^GTACA BssSI 776 CTCGTG CACGAG(-5/-1) BstXI 651 CCACCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 686 CCGTGG C^C[GA][CT]GG EaeI 127 TGGCCA [CT]^GGCC[GA] Eam1105I 793 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 130 CCAAGG C^C[AT][AT]GG FbaI 467 TGATCA T^GATCA Hin1I 696 GGCGCC G[GA]^CG[CT]C HindIII 977 AAGCTT A^AGCTT KasI 696 GGCGCC G^GCGCC MaeIII 871 GTCAC ^GT[ACGT]AC MluNI 127 TGGCCA TGG^CCA MscI 127 TGGCCA TGG^CCA NarI 696 GGCGCC GG^CGCC PflMI 751 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 496 CACGTG CAC^GTG PmlI 496 CACGTG CAC^GTG PpuMI 743 AGGACCT [GA]G^G[AT]CC[CT] SacI 881 GAGCTC GAGCT^C SfcI 247 CTACAG C^T[GA][CT]AG SfoI 696 GGCGCC GGC^GCC SspBI 363 TGTACA T^GTACA StyI 130 CCAAGG C^C[AT][AT]GG Tsp45I 871 GTCAC ^GT[GC]AC Tsp509I 933 AATT ^AATT Van91I 751 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 255 CCAAATCCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 115 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AfaI 364 GTAC GT^AC 1114 GTAC AlwI 384 GATCC GGATC(4/5) 102 GGATC AspHI 551 GTGCAC G[AT]GC[AT]^C 881 GAGCTC AvaI 594 CCCGGG C^[CT]CG[GA]G 875 CCCGGG BbsI 451 GAAGAC GAAGAC(2/6) 1108 GAAGAC BlpI 286 GCTCAGC GC^T[ACGT]AGC 582 GCTGAGC Bpu1102I 286 GCTCAGC GC^T[ACGT]AGC 582 GCTGAGC BpuAI 451 GAAGAC GAAGAC(2/6) 1108 GAAGAC BsgI 182 CTGCAC GTGCAG(16/14) 310 GTGCAG BsiHKAI 551 GTGCAC G[AT]GC[AT]^C 881 GAGCTC BsmFI 436 GGGAC GGGAC(10/14) 1140 GTCCC BsoBI 594 CCCGGG C^[CT]CG[GA]G 875 CCCGGG BsrI 59 ACTGG ACTGG(1/-1) 1125 ACTGG BstYI 102 GGATCT [GA]^GATC[CT] 383 AGATCC CelII 286 GCTCAGC GC^T[ACGT]AGC 582 GCTGAGC CfoI 537 GCGC GCG^C 697 GCGC EcoNI 1047 CCTGACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1071 CCTCATTCAGG HaeII 536 AGCGCC [GA]GCGC^[CT] 696 GGCGCC HhaI 537 GCGC GCG^C 697 GCGC HinP1I 537 GCGC G^CGC 697 GCGC MflI 102 GGATCT [GA]^GATC[CT] 383 AGATCC MslI 456 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 652 CACCTGCATG PleI 438 GACTC GAGTC(4/5) 799 GAGTC PvuII 289 CAGCTG CAG^CTG 1058 CAGCTG RsaI 364 GTAC GT^AC 1114 GTAC SapI 1147 GAAGAGC GCTCTTC(1/4) 4 GCTCTTC SmaI 594 CCCGGG CCC^GGG 875 CCCGGG TfiI 16 GATTC G^A[AT]TC 120 GATTC XhoII 102 GGATCT [GA]^GATC[CT] 383 AGATCC XmaCI 594 CCCGGG C^CCGGG 875 CCCGGG XmaI 594 CCCGGG C^CCGGG 875 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AluI AlwNI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI TspRI