Restriction map for
pF1KB7414
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AvaI AvaII AviII AvrII
BalI BamHI BbrPI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT22I FauI
FbaI FseI FspI HapII HincII
HindII HindIII HpaI HpaII KpnI
Ksp632I KspI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 1067 GAATTC [GA]^AATT[CT]
AcyI 17 GGCGCC G[GA]^CG[CT]C
ApaI 98 GGGCCC GGGCC^C
ApoI 1067 GAATTC [GA]^AATT[CT]
AspI 429 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 98 GGGCCC G[GA]GC[CT]^C
BbeI 17 GGCGCC GGCGC^C
BbvCI 461 CCTCAGC CCTCAGC(-5/-2)
BmyI 98 GGGCCC G[AGT]GC[ACT]^C
BsaHI 17 GGCGCC G[GA]^CG[CT]C
BsaI 177 GGTCTC GGTCTC(1/5)
BseRI 441 GAGGAG GAGGAG(10/8)
BsgI 91 GTGCAG GTGCAG(16/14)
Bsp1286I 98 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 104 TGTACA T^GTACA
BsrGI 104 TGTACA T^GTACA
CfoI 18 GCGC GCG^C
DraI 754 TTTAAA TTT^AAA
DsaI 205 CCATGG C^C[GA][CT]GG
EcoNI 461 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1067 GAATTC G^AATTC
EcoRV 579 GATATC GAT^ATC
EcoT14I 205 CCATGG C^C[AT][AT]GG
HaeII 17 GGCGCC [GA]GCGC^[CT]
HgaI 533 GCGTC GACGC(5/10)
HhaI 18 GCGC GCG^C
Hin1I 17 GGCGCC G[GA]^CG[CT]C
HinP1I 18 GCGC G^CGC
KasI 17 GGCGCC G^GCGCC
NarI 17 GGCGCC GG^CGCC
NcoI 205 CCATGG C^CATGG
PflFI 429 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1251 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 17 GGCGCC GGC^GCC
SmlI 960 CTCAAG C^T[CT][GA]AG
SspBI 104 TGTACA T^GTACA
SspI 526 AATATT AAT^ATT
StyI 205 CCATGG C^C[AT][AT]GG
SwaI 753 ATTTAAAT ATTT^AAAT
TaqI 215 TCGA T^CGA
Tth111I 429 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 215 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 11 CCGC CCGC(-3/-1)
854 GCGG
AfaI 54 GTAC GT^AC
105 GTAC
AlwI 217 GATCC GGATC(4/5)
306 GGATC
Asp700I 942 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1110 GAAGGCATTC
BanI 17 GGCGCC G^G[CT][GA]CC
470 GGCACC
BbsI 1248 GAAGAC GAAGAC(2/6)
880 GTCTTC
BfaI 397 CTAG C^TAG
569 CTAG
BpuAI 1248 GAAGAC GAAGAC(2/6)
880 GTCTTC
BsmAI 444 GAGAC GTCTC(1/5)
178 GTCTC
BstF5I 42 GGATG GGATG(2/0)
267 GGATG
Cac8I 76 GCCAGC GC[ACGT]^[ACGT]GC
353 GCCTGC
DpnII 217 GATC ^GATC
307 GATC
FokI 42 GGATG GGATG(9/13)
267 GGATG
MaeI 397 CTAG C^TAG
569 CTAG
MboI 217 GATC ^GATC
307 GATC
MspA1I 184 CAGCTG C[AC]G^C[GT]G
1203 CAGCTG
MwoI 133 GCAAATGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
142 GCTTCCCTGGC
NdeII 217 GATC ^GATC
307 GATC
PvuII 184 CAGCTG CAG^CTG
1203 CAGCTG
RsaI 54 GTAC GT^AC
105 GTAC
Sau3AI 217 GATC ^GATC
307 GATC
StuI 479 AGGCCT AGG^CCT
860 AGGCCT
Tsp45I 31 GTGAC ^GT[GC]AC
650 GTCAC
XcmI 164 CCACGCCTGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
406 CCACAAGGTCATTGG
XmnI 942 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1110 GAAGGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI BbvI BpmI BsaJI BsiYI
BslI BsmI BsrI BssKI BstNI
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
MaeIII MboII MnlI MseI MslI
MvaI NlaIII NlaIV PleI Sau96I
ScrFI SfaNI TfiI Tru9I TseI
Tsp509I TspRI