Restriction map for
pF1KB7418
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaWI BseAI BseRI BsgI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
FbaI FseI FspI HindIII HpaI
KpnI MaeI MfeI MluI MroI
MslI MunI NcoI NdeI NheI
NotI NruI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AcsI 1096 AAATTC [GA]^AATT[CT]
Alw44I 795 GTGCAC G^TGCAC
ApaLI 795 GTGCAC G^TGCAC
ApoI 1096 AAATTC [GA]^AATT[CT]
AspI 706 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 115 CCCGGG C^[CT]CG[GA]G
BalI 782 TGGCCA TGG^CCA
BbeI 255 GGCGCC GGCGC^C
BcgI 797 GCACAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 927 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 927 GCTGAGC GC^T[ACGT]AGC
BsaAI 343 TACGTG [CT]AC^GT[GA]
BsaBI 285 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 967 GAGACC GGTCTC(1/5)
BsmBI 59 CGTCTC CGTCTC(1/5)
BsmFI 624 GTCCC GGGAC(10/14)
BsoBI 115 CCCGGG C^[CT]CG[GA]G
BspMI 1081 ACCTGC ACCTGC(4/8)
BsrBI 11 CCGCTC CCGCTC(-3/-3)
BsrDI 578 CATTGC GCAATG(2/0)
BstYI 954 AGATCC [GA]^GATC[CT]
CelII 927 GCTGAGC GC^T[ACGT]AGC
DraIII 484 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 208 GACGCTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 146 CGGCCG C^GGCCG
EarI 442 CTCTTC CTCTTC(1/4)
EclXI 146 CGGCCG C^GGCCG
Eco52I 146 CGGCCG C^GGCCG
EcoNI 675 CCTCTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 575 ATGCAT ATGCA^T
HincII 651 GTTGAC GT[CT]^[GA]AC
HindII 651 GTTGAC GT[CT]^[GA]AC
HinfI 622 GAGTC G^A[ACGT]TC
KasI 255 GGCGCC G^GCGCC
Ksp632I 442 CTCTTC CTCTTC(1/4)
KspI 122 CCGCGG CCGC^GG
MaeIII 559 GTCAC ^GT[ACGT]AC
MamI 285 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 954 AGATCC [GA]^GATC[CT]
MluNI 782 TGGCCA TGG^CCA
MscI 782 TGGCCA TGG^CCA
MseI 875 TTAA T^TAA
NarI 255 GGCGCC GG^CGCC
NlaIII 786 CATG CATG^
NsiI 575 ATGCAT ATGCA^T
PflFI 706 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 483 CCACAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 622 GAGTC GAGTC(4/5)
PpuMI 589 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 757 CAGCTG CAG^CTG
SacI 850 GAGCTC GAGCT^C
SacII 122 CCGCGG CCGC^GG
SfoI 255 GGCGCC GGC^GCC
SmaI 115 CCCGGG CCC^GGG
SmlI 907 CTCAAG C^T[CT][GA]AG
TaqI 641 TCGA T^CGA
Tru9I 875 TTAA T^TAA
Tsp45I 559 GTCAC ^GT[GC]AC
Tsp509I 1097 AATT ^AATT
Tth111I 706 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 641 TCGA T^CGA
Van91I 483 CCACAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 954 AGATCC [GA]^GATC[CT]
XmaCI 115 CCCGGG C^CCGGG
XmaI 115 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 955 GATCC GGATC(4/5)
233 GGATC
AlwNI 514 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
754 CAGCAGCTG
AspHI 795 GTGCAC G[AT]GC[AT]^C
850 GAGCTC
BpmI 1072 CTCCAG CTGGAG(16/14)
220 CTGGAG
BsiEI 146 CGGCCG CG[GA][CT]^CG
270 CGGTCG
BsiHKAI 795 GTGCAC G[AT]GC[AT]^C
850 GAGCTC
BsrFI 141 GCCGGC [GA]^CCGG[CT]
923 GCCGGC
BssSI 1033 CACGAG CACGAG(-5/-1)
805 CTCGTG
Cfr10I 141 GCCGGC [GA]^CCGG[CT]
923 GCCGGC
DsaI 122 CCGCGG C^C[GA][CT]GG
599 CCACGG
EaeI 146 CGGCCG [CT]^GGCC[GA]
782 TGGCCA
MboII 443 TCTTC GAAGA(8/7)
468 TCTTC
NaeI 141 GCCGGC GCC^GGC
923 GCCGGC
NgoMIV 141 GCCGGC G^CCGGC
923 GCCGGC
PstI 583 CTGCAG CTGCA^G
751 CTGCAG
SfcI 583 CTGCAG C^T[GA][CT]AG
751 CTGCAG
StuI 401 AGGCCT AGG^CCT
801 AGGCCT
XcmI 316 CCAGTGGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
770 CCATCCTGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BsaHI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI MaeII MboI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
SfaNI StyI TseI TspRI