Restriction map for
pF1KB7422
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BsrI BssSI
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MfeI MluI MroI
MslI MunI NarI NcoI NdeI
NheI NlaIII NotI NruI NsiI
NspI PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SapI SbfI ScaI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tsp509I TspRI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 30 GTCGAC GT^[AC][GT]AC
AlwNI 205 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 258 GAGCTC G[AT]GC[AT]^C
AvaI 48 CCCGAG C^[CT]CG[GA]G
AvaII 243 GGACC G^G[AT]CC
BalI 62 TGGCCA TGG^CCA
BanI 133 GGCACC G^G[CT][GA]CC
BanII 258 GAGCTC G[GA]GC[CT]^C
BbsI 316 GAAGAC GAAGAC(2/6)
BcgI 15 GCAGACTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 130 CCCGG CC^[GC]GG
BmyI 258 GAGCTC G[AGT]GC[ACT]^C
BpuAI 316 GAAGAC GAAGAC(2/6)
BsiHKAI 258 GAGCTC G[AT]GC[AT]^C
BsoBI 48 CCCGAG C^[CT]CG[GA]G
Bsp1286I 258 GAGCTC G[AGT]GC[ACT]^C
BspMI 245 ACCTGC ACCTGC(4/8)
BsrBI 400 GAGCGG CCGCTC(-3/-3)
BssHII 86 GCGCGC G^CGCGC
BstBI 313 TTCGAA TT^CGAA
BstNI 300 CCTGG CC^[AT]GG
BstYI 152 AGATCC [GA]^GATC[CT]
DdeI 11 CTAAG C^T[ACGT]AG
DraII 44 AGGCCCC [GA]G^G[ACGT]CC[CT]
EagI 263 CGGCCG C^GGCCG
EclXI 263 CGGCCG C^GGCCG
Eco52I 263 CGGCCG C^GGCCG
EcoO109I 44 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 300 CCTGG ^CC[AT]GG
HincII 30 GTCGAC GT[CT]^[GA]AC
HindII 30 GTCGAC GT[CT]^[GA]AC
HphI 105 GGTGA GGTGA(8/7)
MaeIII 352 GTGAC ^GT[ACGT]AC
MflI 152 AGATCC [GA]^GATC[CT]
MluNI 62 TGGCCA TGG^CCA
MscI 62 TGGCCA TGG^CCA
MseI 236 TTAA T^TAA
MvaI 300 CCTGG CC^[AT]GG
NaeI 90 GCCGGC GCC^GGC
NciI 130 CCCGG CC^[GC]GG
NgoMIV 90 GCCGGC G^CCGGC
NspV 313 TTCGAA TT^CGAA
PflMI 204 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 277 CTGCAG CTGCA^G
SacI 258 GAGCTC GAGCT^C
SalI 30 GTCGAC G^TCGAC
SexAI 299 ACCTGGT A^CC[AT]GGT
SfaNI 389 GCATC GCATC(5/9)
SfcI 277 CTGCAG C^T[GA][CT]AG
SfiI 285 GGCCAGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 313 TTCGAA TT^CGAA
StuI 293 AGGCCT AGG^CCT
Tru9I 236 TTAA T^TAA
Tsp45I 352 GTGAC ^GT[GC]AC
Van91I 204 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 152 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 8 GTAC GT^AC
214 GTAC
AlwI 153 GATCC GGATC(4/5)
186 GATCC
BsiEI 32 CGACCG CG[GA][CT]^CG
263 CGGCCG
BssKI 130 CCCGG ^CC[ACGT]GG
300 CCTGG
EcoT14I 68 CCAAGG C^C[AT][AT]GG
365 CCAAGG
HaeII 158 GGCGCT [GA]GCGC^[CT]
273 GGCGCT
MboII 108 GAAGA GAAGA(8/7)
316 GAAGA
NlaIV 45 GGCCCC GG[ACGT]^[ACGT]CC
133 GGCACC
PvuII 58 CAGCTG CAG^CTG
376 CAGCTG
RsaI 8 GTAC GT^AC
214 GTAC
ScrFI 130 CCCGG CC^[ACGT]GG
300 CCTGG
StyI 68 CCAAGG C^C[AT][AT]GG
365 CCAAGG
TaqI 31 TCGA T^CGA
314 TCGA
TthHB8I 31 TCGA T^CGA
314 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AluI BbvI BglI
BsaJI BsiYI BslI BsrFI BstF5I
BstUI Cac8I CfoI Cfr10I Cfr13I
DpnII DsaI EaeI FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MboI MnlI MspA1I
MspI MvnI MwoI NdeII Sau3AI
Sau96I TseI