Restriction map for
pF1KB7425
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BfrI BlnI BsaAI BsaI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstUI BstXI Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HaeII
HindIII HpaI KasI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tsp45I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 944 GGTACC G^GTACC
AcyI 213 GACGCC G[GA]^CG[CT]C
Alw44I 989 GTGCAC G^TGCAC
AlwI 1346 GGATC GGATC(4/5)
ApaLI 989 GTGCAC G^TGCAC
Asp718I 944 GGTACC G^GTACC
AvaI 1244 CTCGGG C^[CT]CG[GA]G
BalI 68 TGGCCA TGG^CCA
BbsI 210 GAAGAC GAAGAC(2/6)
BciVI 617 GTATCC GTATCC(6/5)
BclI 860 TGATCA T^GATCA
BcnI 941 CCCGG CC^[GC]GG
BglI 1196 GCCACACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 210 GAAGAC GAAGAC(2/6)
BsaBI 481 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 213 GACGCC G[GA]^CG[CT]C
BsaWI 1359 TCCGGT [AT]^CCGG[AT]
BsgI 166 GTGCAG GTGCAG(16/14)
BsmI 572 GCATTC GAATGC(1/-1)
BsoBI 1244 CTCGGG C^[CT]CG[GA]G
BspLU11I 641 ACATGT A^CATGT
BsrDI 1157 GCAATG GCAATG(2/0)
BsrI 1401 ACTGG ACTGG(1/-1)
DrdI 339 GACCATATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1169 CCATGG C^C[GA][CT]GG
EcoRI 772 GAATTC G^AATTC
FauI 315 GCGGG CCCGC(4/6)
FbaI 860 TGATCA T^GATCA
Hin1I 213 GACGCC G[GA]^CG[CT]C
HincII 959 GTCAAC GT[CT]^[GA]AC
HindII 959 GTCAAC GT[CT]^[GA]AC
KpnI 944 GGTACC GGTAC^C
MamI 481 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 68 TGGCCA TGG^CCA
MscI 68 TGGCCA TGG^CCA
MseI 898 TTAA T^TAA
NciI 941 CCCGG CC^[GC]GG
NcoI 1169 CCATGG C^CATGG
NdeI 342 CATATG CA^TATG
NspI 641 ACATGT [GA]CATG^[CT]
PshAI 1261 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 102 GCTCTTC GCTCTTC(1/4)
SfcI 7 CTACAG C^T[GA][CT]AG
Tru9I 898 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 604 GTATAC GT^[AC][GT]AC
1316 GTATAC
AciI 291 GCGG CCGC(-3/-1)
315 GCGG
AflIII 641 ACATGT A^C[GA][CT]GT
1163 ACACGT
AlwNI 43 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
138 CAGACGCTG
AocI 789 CCTCAGG CC^T[ACGT]AGG
870 CCTCAGG
Asp700I 441 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
521 GAAGAGGTTC
BanI 866 GGTGCC G^G[CT][GA]CC
944 GGTACC
BglII 227 AGATCT A^GATCT
486 AGATCT
BseRI 787 CTCCTC GAGGAG(10/8)
1285 CTCCTC
BsmAI 886 GAGAC GTCTC(1/5)
953 GTCTC
BspMI 734 ACCTGC ACCTGC(4/8)
1187 ACCTGC
Bst1107I 604 GTATAC GTA^TAC
1316 GTATAC
BstAPI 42 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
137 GCAGACGCTGC
BstZ17I 604 GTATAC GTA^TAC
1316 GTATAC
Bsu36I 789 CCTCAGG CC^T[ACGT]AGG
870 CCTCAGG
CfoI 996 GCGC GCG^C
1194 GCGC
EaeI 68 TGGCCA [CT]^GGCC[GA]
649 TGGCCG
Eco81I 789 CCTCAGG CC^T[ACGT]AGG
870 CCTCAGG
HapII 942 CCGG C^CGG
1360 CCGG
HgaI 140 GACGC GACGC(5/10)
213 GACGC
HhaI 996 GCGC GCG^C
1194 GCGC
HinP1I 996 GCGC G^CGC
1194 GCGC
HpaII 942 CCGG C^CGG
1360 CCGG
MaeII 1165 ACGT A^CGT
1384 ACGT
MaeIII 677 GTTAC ^GT[ACGT]AC
731 GTTAC
MslI 1153 CACGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1164 CACGTCCATG
MspA1I 856 CAGCTG C[AC]G^C[GT]G
970 CAGCTG
MspI 942 CCGG C^CGG
1360 CCGG
PflMI 60 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
621 CCAAGTGTTGG
PpuMI 199 GGGTCCC [GA]G^G[AT]CC[CT]
1184 AGGACCT
PvuII 856 CAGCTG CAG^CTG
970 CAGCTG
SfaNI 810 GATGC GCATC(5/9)
1311 GATGC
SmlI 463 CTCAAG C^T[CT][GA]AG
1009 CTTGAG
Van91I 60 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
621 CCAAGTGTTGG
XcmI 71 CCATCTCCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1391 CCACTGAGCAACTGG
XmnI 441 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
521 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
AvaII BanII BbvI BfaI BlpI
BmyI BpmI Bpu1102I BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI BstYI Cac8I CelII
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MboI MboII MflI MnlI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI SacI Sau3AI Sau96I
ScrFI StuI StyI TfiI TseI
Tsp509I TspRI XhoII