################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII AccII AccIII AclI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BfrI BlnI BsaAI BsaI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI BstXI Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HaeII HindIII HpaI KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 944 GGTACC G^GTACC AcyI 213 GACGCC G[GA]^CG[CT]C Alw44I 989 GTGCAC G^TGCAC AlwI 1346 GGATC GGATC(4/5) ApaLI 989 GTGCAC G^TGCAC Asp718I 944 GGTACC G^GTACC AvaI 1244 CTCGGG C^[CT]CG[GA]G BalI 68 TGGCCA TGG^CCA BbsI 210 GAAGAC GAAGAC(2/6) BciVI 617 GTATCC GTATCC(6/5) BclI 860 TGATCA T^GATCA BcnI 941 CCCGG CC^[GC]GG BglI 1196 GCCACACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 210 GAAGAC GAAGAC(2/6) BsaBI 481 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 213 GACGCC G[GA]^CG[CT]C BsaWI 1359 TCCGGT [AT]^CCGG[AT] BsgI 166 GTGCAG GTGCAG(16/14) BsmI 572 GCATTC GAATGC(1/-1) BsoBI 1244 CTCGGG C^[CT]CG[GA]G BspLU11I 641 ACATGT A^CATGT BsrDI 1157 GCAATG GCAATG(2/0) BsrI 1401 ACTGG ACTGG(1/-1) DrdI 339 GACCATATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1169 CCATGG C^C[GA][CT]GG EcoRI 772 GAATTC G^AATTC FauI 315 GCGGG CCCGC(4/6) FbaI 860 TGATCA T^GATCA Hin1I 213 GACGCC G[GA]^CG[CT]C HincII 959 GTCAAC GT[CT]^[GA]AC HindII 959 GTCAAC GT[CT]^[GA]AC KpnI 944 GGTACC GGTAC^C MamI 481 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 68 TGGCCA TGG^CCA MscI 68 TGGCCA TGG^CCA MseI 898 TTAA T^TAA NciI 941 CCCGG CC^[GC]GG NcoI 1169 CCATGG C^CATGG NdeI 342 CATATG CA^TATG NspI 641 ACATGT [GA]CATG^[CT] PshAI 1261 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 102 GCTCTTC GCTCTTC(1/4) SfcI 7 CTACAG C^T[GA][CT]AG Tru9I 898 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccI 604 GTATAC GT^[AC][GT]AC 1316 GTATAC AciI 291 GCGG CCGC(-3/-1) 315 GCGG AflIII 641 ACATGT A^C[GA][CT]GT 1163 ACACGT AlwNI 43 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 138 CAGACGCTG AocI 789 CCTCAGG CC^T[ACGT]AGG 870 CCTCAGG Asp700I 441 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 521 GAAGAGGTTC BanI 866 GGTGCC G^G[CT][GA]CC 944 GGTACC BglII 227 AGATCT A^GATCT 486 AGATCT BseRI 787 CTCCTC GAGGAG(10/8) 1285 CTCCTC BsmAI 886 GAGAC GTCTC(1/5) 953 GTCTC BspMI 734 ACCTGC ACCTGC(4/8) 1187 ACCTGC Bst1107I 604 GTATAC GTA^TAC 1316 GTATAC BstAPI 42 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 137 GCAGACGCTGC BstZ17I 604 GTATAC GTA^TAC 1316 GTATAC Bsu36I 789 CCTCAGG CC^T[ACGT]AGG 870 CCTCAGG CfoI 996 GCGC GCG^C 1194 GCGC EaeI 68 TGGCCA [CT]^GGCC[GA] 649 TGGCCG Eco81I 789 CCTCAGG CC^T[ACGT]AGG 870 CCTCAGG HapII 942 CCGG C^CGG 1360 CCGG HgaI 140 GACGC GACGC(5/10) 213 GACGC HhaI 996 GCGC GCG^C 1194 GCGC HinP1I 996 GCGC G^CGC 1194 GCGC HpaII 942 CCGG C^CGG 1360 CCGG MaeII 1165 ACGT A^CGT 1384 ACGT MaeIII 677 GTTAC ^GT[ACGT]AC 731 GTTAC MslI 1153 CACGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1164 CACGTCCATG MspA1I 856 CAGCTG C[AC]G^C[GT]G 970 CAGCTG MspI 942 CCGG C^CGG 1360 CCGG PflMI 60 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 621 CCAAGTGTTGG PpuMI 199 GGGTCCC [GA]G^G[AT]CC[CT] 1184 AGGACCT PvuII 856 CAGCTG CAG^CTG 970 CAGCTG SfaNI 810 GATGC GCATC(5/9) 1311 GATGC SmlI 463 CTCAAG C^T[CT][GA]AG 1009 CTTGAG Van91I 60 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 621 CCAAGTGTTGG XcmI 71 CCATCTCCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1391 CCACTGAGCAACTGG XmnI 441 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 521 GAAGAGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AcsI AfaI AluI ApoI AspHI AvaII BanII BbvI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MboI MboII MflI MnlI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI SacI Sau3AI Sau96I ScrFI StuI StyI TfiI TseI Tsp509I TspRI XhoII