Restriction map for
pF1KB7431
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
Acc65I AccI AccII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BfrI BglII BlnI BsaAI BsaBI
BsaI BsiHKAI BsiWI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraI DraIII DsaI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MluI
MluNI MscI MslI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tsp45I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 897 GACGTC GACGT^C
AccIII 714 TCCGGA T^CCGGA
AlwNI 314 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 7 CCTGAGG CC^T[ACGT]AGG
ApaI 301 GGGCCC GGGCC^C
AvaII 834 GGACC G^G[AT]CC
BbeI 445 GGCGCC GGCGC^C
BbsI 532 GAAGAC GAAGAC(2/6)
BciVI 711 GTATCC GTATCC(6/5)
BclI 515 TGATCA T^GATCA
BfaI 433 CTAG C^TAG
BlpI 540 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 540 GCTGAGC GC^T[ACGT]AGC
BpuAI 532 GAAGAC GAAGAC(2/6)
BsaWI 714 TCCGGA [AT]^CCGG[AT]
BseAI 714 TCCGGA T^CCGGA
BsiEI 146 CGGCCG CG[GA][CT]^CG
BsmAI 895 GAGAC GTCTC(1/5)
BsmBI 895 GAGACG CGTCTC(1/5)
BspEI 714 TCCGGA T^CCGGA
BsrFI 360 GCCGGT [GA]^CCGG[CT]
BssSI 247 CTCGTG CACGAG(-5/-1)
BstAPI 282 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 700 CCAATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 7 CCTGAGG CC^T[ACGT]AGG
CelII 540 GCTGAGC GC^T[ACGT]AGC
Cfr10I 360 GCCGGT [GA]^CCGG[CT]
DrdI 309 GACCCCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 146 CGGCCG C^GGCCG
EclXI 146 CGGCCG C^GGCCG
Eco52I 146 CGGCCG C^GGCCG
Eco81I 7 CCTGAGG CC^T[ACGT]AGG
EcoNI 825 CCTATGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 515 TGATCA T^GATCA
HaeII 445 GGCGCC [GA]GCGC^[CT]
HgaI 461 GCGTC GACGC(5/10)
HphI 663 GGTGA GGTGA(8/7)
KasI 445 GGCGCC G^GCGCC
MaeI 433 CTAG C^TAG
MaeII 898 ACGT A^CGT
MaeIII 620 GTAAC ^GT[ACGT]AC
MfeI 701 CAATTG C^AATTG
MroI 714 TCCGGA T^CCGGA
MunI 701 CAATTG C^AATTG
NarI 445 GGCGCC GG^CGCC
NspI 536 ACATGC [GA]CATG^[CT]
PpuMI 833 AGGACCT [GA]G^G[AT]CC[CT]
SfaNI 579 GCATC GCATC(5/9)
SfoI 445 GGCGCC GGC^GCC
Tsp509I 702 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 445 GGCGCC G[GA]^CG[CT]C
897 GACGTC
AlwI 822 GATCC GGATC(4/5)
802 GGATC
BanII 12 GGGCTC G[GA]GC[CT]^C
301 GGGCCC
BcnI 27 CCGGG CC^[GC]GG
149 CCGGG
BsaHI 445 GGCGCC G[GA]^CG[CT]C
897 GACGTC
BsgI 320 CTGCAC GTGCAG(16/14)
133 GTGCAG
BsmFI 905 GGGAC GGGAC(10/14)
463 GTCCC
BsrBI 253 GAGCGG CCGCTC(-3/-3)
412 GAGCGG
BstYI 802 GGATCT [GA]^GATC[CT]
821 AGATCC
DraII 242 AGGGCCT [GA]G^G[ACGT]CC[CT]
833 AGGACCT
EaeI 146 CGGCCG [CT]^GGCC[GA]
338 TGGCCG
EarI 571 GAAGAG CTCTTC(1/4)
883 GAAGAG
EcoO109I 242 AGGGCCT [GA]G^G[ACGT]CC[CT]
833 AGGACCT
Hin1I 445 GGCGCC G[GA]^CG[CT]C
897 GACGTC
HinfI 316 GAGTC G^A[ACGT]TC
907 GACTC
Ksp632I 571 GAAGAG CTCTTC(1/4)
883 GAAGAG
MflI 802 GGATCT [GA]^GATC[CT]
821 AGATCC
MseI 365 TTAA T^TAA
764 TTAA
MspA1I 271 CAGCGG C[AC]G^C[GT]G
397 CAGCGG
NciI 27 CCGGG CC^[GC]GG
149 CCGGG
PleI 907 GACTC GAGTC(4/5)
316 GAGTC
Tru9I 365 TTAA T^TAA
764 TTAA
TspRI 323 CACTG CA[GC]TG[ACGT][ACGT]^
519 CACTG
XcmI 456 CCAGAGCGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
650 CCAAGGAATATCTGG
XhoII 802 GGATCT [GA]^GATC[CT]
821 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI BanI BbvI BglI
BmyI BpmI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI StuI
StyI TseI