Restriction map for
pF1KB7432
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI AluI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BbvI
BcgI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI FauI
Fnu4HI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII ItaI
KasI KpnI KspI MaeI MaeII
MfeI MflI MluI MroI MspA1I
MspI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI TseI Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 106 GTCTAC GT^[AC][GT]AC
AccII 483 CGCG CG^CG
AcsI 77 AAATTC [GA]^AATT[CT]
AfaI 787 GTAC GT^AC
AlwI 849 GATCC GGATC(4/5)
ApoI 77 AAATTC [GA]^AATT[CT]
AseI 339 ATTAAT AT^TAAT
AsnI 339 ATTAAT AT^TAAT
AspHI 486 GTGCTC G[AT]GC[AT]^C
BalI 377 TGGCCA TGG^CCA
BbsI 715 GTCTTC GAAGAC(2/6)
BciVI 957 GTATCC GTATCC(6/5)
BclI 146 TGATCA T^GATCA
BpmI 804 CTCCAG CTGGAG(16/14)
BpuAI 715 GTCTTC GAAGAC(2/6)
BsaBI 141 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 486 GTGCTC G[AT]GC[AT]^C
BsiYI 369 CCACTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 369 CCACTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 661 GCATTC GAATGC(1/-1)
BsrDI 234 CATTGC GCAATG(2/0)
BstUI 483 CGCG CG^CG
Cac8I 484 GCGTGC GC[ACGT]^[ACGT]GC
DraI 495 TTTAAA TTT^AAA
DraIII 370 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 377 TGGCCA [CT]^GGCC[GA]
EcoRV 558 GATATC GAT^ATC
EcoT14I 759 CCATGG C^C[AT][AT]GG
FbaI 146 TGATCA T^GATCA
MamI 141 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 377 TGGCCA TGG^CCA
MscI 377 TGGCCA TGG^CCA
MvnI 483 CGCG CG^CG
NcoI 759 CCATGG C^CATGG
NspI 466 GCATGT [GA]CATG^[CT]
PflMI 369 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 339 ATTAAT AT^TAAT
RsaI 787 GTAC GT^AC
SexAI 257 ACCTGGT A^CC[AT]GGT
SfcI 873 CTACAG C^T[GA][CT]AG
SspI 929 AATATT AAT^ATT
StyI 759 CCATGG C^C[AT][AT]GG
Van91I 369 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 708 CCGC CCGC(-3/-1)
352 GCGG
Asp700I 275 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
337 GAATTAATTC
BanI 704 GGCACC G^G[CT][GA]CC
763 GGCACC
BmyI 486 GTGCTC G[AGT]GC[ACT]^C
703 GGGCAC
BsaJI 759 CCATGG C^C[ACGT][ACGT]GG
842 CCGTGG
Bsp1286I 486 GTGCTC G[AGT]GC[ACT]^C
703 GGGCAC
BsrI 591 CCAGT ACTGG(1/-1)
698 CCAGT
BstXI 242 CCATGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
431 CCATGCTGGTGG
DsaI 759 CCATGG C^C[GA][CT]GG
842 CCGTGG
EarI 85 CTCTTC CTCTTC(1/4)
772 CTCTTC
EcoT22I 452 ATGCAT ATGCA^T
659 ATGCAT
Ksp632I 85 CTCTTC CTCTTC(1/4)
772 CTCTTC
MaeIII 331 GTGAC ^GT[ACGT]AC
673 GTCAC
MwoI 352 GCGGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
484 GCGTGCTCTGC
NlaIV 704 GGCACC GG[ACGT]^[ACGT]CC
763 GGCACC
NsiI 452 ATGCAT ATGCA^T
659 ATGCAT
PleI 577 GACTC GAGTC(4/5)
461 GAGTC
TfiI 129 GAATC G^A[AT]TC
861 GAATC
Tsp45I 331 GTGAC ^GT[GC]AC
673 GTCAC
XmnI 275 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
337 GAATTAATTC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
BssKI BstF5I BstNI DdeI DpnII
EcoRII FokI HaeIII HinfI HphI
MboI MboII MnlI MseI MslI
MvaI NdeII NlaIII Sau3AI ScrFI
SfaNI Tru9I Tsp509I TspRI