Restriction map for
pF1KB7433
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BspHI BspMI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI KspI MaeII
MamI MflI MluI MroI MseI
MspI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI Tru9I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcsI 249 GAATTT [GA]^AATT[CT]
AflIII 653 ACATGT A^C[GA][CT]GT
AlwI 135 GATCC GGATC(4/5)
ApoI 249 GAATTT [GA]^AATT[CT]
AspHI 684 GAGCTC G[AT]GC[AT]^C
BalI 371 TGGCCA TGG^CCA
BanI 757 GGCACC G^G[CT][GA]CC
BanII 684 GAGCTC G[GA]GC[CT]^C
BbvI 305 GCTGC GCAGC(8/12)
BciVI 153 GGATAC GTATCC(6/5)
BfaI 196 CTAG C^TAG
BlpI 686 GCTCAGC GC^T[ACGT]AGC
BmyI 684 GAGCTC G[AGT]GC[ACT]^C
BpmI 410 CTCCAG CTGGAG(16/14)
Bpu1102I 686 GCTCAGC GC^T[ACGT]AGC
BseRI 550 CTCCTC GAGGAG(10/8)
BsiHKAI 684 GAGCTC G[AT]GC[AT]^C
BsmI 422 GCATTC GAATGC(1/-1)
Bsp1286I 684 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 257 TGTACA T^GTACA
BspLU11I 653 ACATGT A^CATGT
BsrBI 149 GAGCGG CCGCTC(-3/-3)
BsrGI 257 TGTACA T^GTACA
Cac8I 735 GCTGGC GC[ACGT]^[ACGT]GC
CelII 686 GCTCAGC GC^T[ACGT]AGC
Cfr13I 45 GGGCC G^G[ACGT]CC
DsaI 350 CCATGG C^C[GA][CT]GG
EaeI 371 TGGCCA [CT]^GGCC[GA]
EcoT14I 350 CCATGG C^C[AT][AT]GG
EcoT22I 284 ATGCAT ATGCA^T
Fnu4HI 305 GCTGC GC^[ACGT]GC
HgaI 701 GACGC GACGC(5/10)
HincII 520 GTCAAC GT[CT]^[GA]AC
HindII 520 GTCAAC GT[CT]^[GA]AC
ItaI 305 GCTGC GC^[ACGT]GC
MaeI 196 CTAG C^TAG
MfeI 12 CAATTG C^AATTG
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MspA1I 689 CAGCTG C[AC]G^C[GT]G
MunI 12 CAATTG C^AATTG
MwoI 346 GCTTCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 350 CCATGG C^CATGG
NlaIV 757 GGCACC GG[ACGT]^[ACGT]CC
NsiI 284 ATGCAT ATGCA^T
PleI 408 GACTC GAGTC(4/5)
PvuII 689 CAGCTG CAG^CTG
SacI 684 GAGCTC GAGCT^C
Sau96I 45 GGGCC G^G[ACGT]CC
SmlI 789 CTCAAG C^T[CT][GA]AG
SspBI 257 TGTACA T^GTACA
StyI 350 CCATGG C^C[AT][AT]GG
TseI 305 GCTGC G^C[AT]GC
Tsp45I 742 GTCAC ^GT[GC]AC
TspRI 113 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 492 CCGC CCGC(-3/-1)
151 GCGG
AocI 552 CCTCAGG CC^T[ACGT]AGG
675 CCTGAGG
BbsI 698 GAAGAC GAAGAC(2/6)
826 GTCTTC
BpuAI 698 GAAGAC GAAGAC(2/6)
826 GTCTTC
BsaJI 20 CCCAGG C^C[ACGT][ACGT]GG
350 CCATGG
BsiYI 363 CCGATATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
671 CCATCCTGAGG
BslI 363 CCGATATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
671 CCATCCTGAGG
BsrI 300 ACTGG ACTGG(1/-1)
342 ACTGG
BssKI 21 CCAGG ^CC[ACGT]GG
412 CCAGG
BstNI 21 CCAGG CC^[AT]GG
412 CCAGG
Bsu36I 552 CCTCAGG CC^T[ACGT]AGG
675 CCTGAGG
DpnII 135 GATC ^GATC
765 GATC
EarI 106 CTCTTC CTCTTC(1/4)
219 CTCTTC
Eco81I 552 CCTCAGG CC^T[ACGT]AGG
675 CCTGAGG
EcoRII 21 CCAGG ^CC[AT]GG
412 CCAGG
Ksp632I 106 CTCTTC CTCTTC(1/4)
219 CTCTTC
MboI 135 GATC ^GATC
765 GATC
MslI 168 CACACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
315 CACCTTCATG
MvaI 21 CCAGG CC^[AT]GG
412 CCAGG
NdeII 135 GATC ^GATC
765 GATC
Sau3AI 135 GATC ^GATC
765 GATC
ScrFI 21 CCAGG CC^[ACGT]GG
412 CCAGG
SfcI 450 CTATAG C^T[GA][CT]AG
720 CTGTAG
Tsp509I 13 AATT ^AATT
250 AATT
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
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AfaI AluI BsmAI BstF5I DdeI
FokI HaeIII HinfI HphI MaeIII
MboII MnlI NlaIII NspI RsaI
SfaNI TfiI