Restriction map for
pF1KB7435
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MaeII MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI NarI NciI NcoI
NdeI NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AcsI 74 GAATTC [GA]^AATT[CT]
AflIII 229 ACATGT A^C[GA][CT]GT
ApoI 74 GAATTC [GA]^AATT[CT]
AspHI 31 GAGCTC G[AT]GC[AT]^C
BanII 31 GAGCTC G[GA]GC[CT]^C
BbvI 357 GCAGC GCAGC(8/12)
BfaI 142 CTAG C^TAG
BglI 124 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 31 GAGCTC G[AGT]GC[ACT]^C
BsaBI 82 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 28 GAGGAG GAGGAG(10/8)
BsgI 58 GTGCAG GTGCAG(16/14)
BsiHKAI 31 GAGCTC G[AT]GC[AT]^C
BsmAI 367 GTCTC GTCTC(1/5)
BsmI 271 GCATTC GAATGC(1/-1)
Bsp1286I 31 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 229 ACATGT A^CATGT
BsrFI 15 GCCGGC [GA]^CCGG[CT]
BssHII 68 GCGCGC G^CGCGC
BstAPI 299 GCATCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 300 CATCC GGATG(2/0)
BstXI 198 CCAGGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 15 GCCGGC [GA]^CCGG[CT]
DpnII 148 GATC ^GATC
DraIII 176 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2 TGGCCG [CT]^GGCC[GA]
EarI 288 GAAGAG CTCTTC(1/4)
EcoNI 330 CCTGAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 74 GAATTC G^AATTC
FokI 300 CATCC GGATG(9/13)
HapII 16 CCGG C^CGG
HincII 381 GTTGAC GT[CT]^[GA]AC
HindII 381 GTTGAC GT[CT]^[GA]AC
HinfI 333 GAGTC G^A[ACGT]TC
HpaII 16 CCGG C^CGG
Ksp632I 288 GAAGAG CTCTTC(1/4)
MaeI 142 CTAG C^TAG
MaeIII 293 GTGAC ^GT[ACGT]AC
MamI 82 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 148 GATC ^GATC
MseI 166 TTAA T^TAA
MslI 224 CATACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 16 CCGG C^CGG
NaeI 15 GCCGGC GCC^GGC
NdeII 148 GATC ^GATC
NgoMIV 15 GCCGGC G^CCGGC
NspI 229 ACATGT [GA]CATG^[CT]
PflMI 129 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 333 GAGTC GAGTC(4/5)
SacI 31 GAGCTC GAGCT^C
Sau3AI 148 GATC ^GATC
SexAI 197 ACCAGGT A^CC[AT]GGT
StuI 131 AGGCCT AGG^CCT
TaqI 150 TCGA T^CGA
Tru9I 166 TTAA T^TAA
TseI 357 GCAGC G^C[AT]GC
Tsp45I 293 GTGAC ^GT[GC]AC
Tsp509I 75 AATT ^AATT
TthHB8I 150 TCGA T^CGA
Van91I 129 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 125 CCACCCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 69 CGCG CG^CG
71 CGCG
AluI 32 AGCT AG^CT
359 AGCT
BsrI 179 CCAGT ACTGG(1/-1)
215 CCAGT
BssKI 129 CCAGG ^CC[ACGT]GG
198 CCAGG
BstNI 129 CCAGG CC^[AT]GG
198 CCAGG
BstUI 69 CGCG CG^CG
71 CGCG
CfoI 68 GCGC GCG^C
70 GCGC
DraII 200 AGGTCCT [GA]G^G[ACGT]CC[CT]
346 AGGACCT
EcoO109I 200 AGGTCCT [GA]G^G[ACGT]CC[CT]
346 AGGACCT
EcoRII 129 CCAGG ^CC[AT]GG
198 CCAGG
EcoT14I 134 CCTTGG C^C[AT][AT]GG
204 CCTTGG
HaeIII 3 GGCC GG^CC
132 GGCC
HhaI 68 GCGC GCG^C
70 GCGC
HinP1I 68 GCGC G^CGC
70 GCGC
HphI 161 TCACC GGTGA(8/7)
175 TCACC
MboII 288 GAAGA GAAGA(8/7)
405 GAAGA
MnlI 28 GAGG CCTC(7/6)
345 GAGG
MvaI 129 CCAGG CC^[AT]GG
198 CCAGG
MvnI 69 CGCG CG^CG
71 CGCG
MwoI 124 GCCACCCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
299 GCATCCTGTGC
NlaIII 230 CATG CATG^
257 CATG
PpuMI 200 AGGTCCT [GA]G^G[AT]CC[CT]
346 AGGACCT
ScrFI 129 CCAGG CC^[ACGT]GG
198 CCAGG
SfaNI 299 GCATC GCATC(5/9)
312 GCATC
StyI 134 CCTTGG C^C[AT][AT]GG
204 CCTTGG
TspRI 180 CAGTG CA[GC]TG[ACGT][ACGT]^
278 CAGTG
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AciI AvaII BsaJI BsiYI BslI
Cac8I Cfr13I DdeI Fnu4HI ItaI
Sau96I