Restriction map for
pF1KB7436
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI Fnu4HI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII HphI ItaI KasI
KpnI Ksp632I KspI MaeI MaeIII
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TfiI TseI Tsp45I Tth111I
Van91I XbaI XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AciI 230 CCGC CCGC(-3/-1)
AcsI 251 AAATTT [GA]^AATT[CT]
Alw44I 286 GTGCAC G^TGCAC
AlwI 159 GGATC GGATC(4/5)
ApaLI 286 GTGCAC G^TGCAC
ApoI 251 AAATTT [GA]^AATT[CT]
AspHI 286 GTGCAC G[AT]GC[AT]^C
AvaI 379 CTCGAG C^[CT]CG[GA]G
BalI 420 TGGCCA TGG^CCA
BbsI 436 GAAGAC GAAGAC(2/6)
BpuAI 436 GAAGAC GAAGAC(2/6)
BsiHKAI 286 GTGCAC G[AT]GC[AT]^C
BsiYI 276 CCACCAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 276 CCACCAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 410 GGGAC GGGAC(10/14)
BsoBI 379 CTCGAG C^[CT]CG[GA]G
BspHI 429 TCATGA T^CATGA
BspMI 332 GCAGGT ACCTGC(4/8)
BsrI 10 ACTGG ACTGG(1/-1)
BstAPI 56 GCACTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 70 GGATG GGATG(2/0)
BstYI 159 GGATCT [GA]^GATC[CT]
Cac8I 308 GCCTGC GC[ACGT]^[ACGT]GC
DpnII 160 GATC ^GATC
DraIII 215 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 390 CCGTGG C^C[GA][CT]GG
EaeI 420 TGGCCA [CT]^GGCC[GA]
EcoT14I 267 CCAAGG C^C[AT][AT]GG
FokI 70 GGATG GGATG(9/13)
HinfI 169 GACTC G^A[ACGT]TC
MaeII 425 ACGT A^CGT
MboI 160 GATC ^GATC
MflI 159 GGATCT [GA]^GATC[CT]
MluNI 420 TGGCCA TGG^CCA
MscI 420 TGGCCA TGG^CCA
MslI 424 CACGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 160 GATC ^GATC
NlaIV 4 GGAGCC GG[ACGT]^[ACGT]CC
NspI 107 GCATGT [GA]CATG^[CT]
PaeR7I 379 CTCGAG C^TCGAG
PleI 169 GACTC GAGTC(4/5)
RcaI 429 TCATGA T^CATGA
Sau3AI 160 GATC ^GATC
SspI 76 AATATT AAT^ATT
StyI 267 CCAAGG C^C[AT][AT]GG
TaqI 380 TCGA T^CGA
TthHB8I 380 TCGA T^CGA
XhoI 379 CTCGAG C^TCGAG
XhoII 159 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
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AluI 294 AGCT AG^CT
361 AGCT
BmyI 286 GTGCAC G[AGT]GC[ACT]^C
401 GGGCAC
BpmI 33 CTGGAG CTGGAG(16/14)
180 CTGGAG
BsmAI 36 GAGAC GTCTC(1/5)
94 GAGAC
Bsp1286I 286 GTGCAC G[AGT]GC[ACT]^C
401 GGGCAC
MseI 164 TTAA T^TAA
239 TTAA
MwoI 56 GCACTGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
65 GCCAGGGATGC
NlaIII 108 CATG CATG^
430 CATG
SfaNI 71 GATGC GCATC(5/9)
298 GATGC
SmlI 139 CTTGAG C^T[CT][GA]AG
379 CTCGAG
Tru9I 164 TTAA T^TAA
239 TTAA
Tsp509I 46 AATT ^AATT
252 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BsaJI BssKI BstNI DdeI EcoRII
HaeIII MboII MnlI MvaI ScrFI
TspRI