Restriction map for
pF1KB7437
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmAI BsmBI BsmFI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrI BssHII BssSI BstAPI
BstBI BstEII BstNI BstPI BstUI
BstXI Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MunI MvaI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 20 GGTACC G^GTACC
AcsI 451 AAATTT [GA]^AATT[CT]
Alw44I 82 GTGCAC G^TGCAC
AlwI 235 GGATC GGATC(4/5)
ApaLI 82 GTGCAC G^TGCAC
ApoI 451 AAATTT [GA]^AATT[CT]
Asp700I 438 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 20 GGTACC G^GTACC
AspHI 82 GTGCAC G[AT]GC[AT]^C
BanI 20 GGTACC G^G[CT][GA]CC
BanII 557 GAGCCC G[GA]GC[CT]^C
BciVI 279 GTATCC GTATCC(6/5)
BcnI 17 CCCGG CC^[GC]GG
BsaAI 628 TACGTA [CT]AC^GT[GA]
BseRI 30 CTCCTC GAGGAG(10/8)
BsgI 676 CTGCAC GTGCAG(16/14)
BsiEI 35 CGATCG CG[GA][CT]^CG
BsiHKAI 82 GTGCAC G[AT]GC[AT]^C
BsmI 119 GCATTC GAATGC(1/-1)
Bsp1407I 195 TGTACA T^GTACA
BspMI 132 GCAGGT ACCTGC(4/8)
BsrGI 195 TGTACA T^GTACA
BssKI 17 CCCGG ^CC[ACGT]GG
Bst1107I 420 GTATAC GTA^TAC
BstF5I 87 CATCC GGATG(2/0)
BstYI 235 GGATCT [GA]^GATC[CT]
BstZ17I 420 GTATAC GTA^TAC
Cac8I 375 GCAAGC GC[ACGT]^[ACGT]GC
DraI 264 TTTAAA TTT^AAA
EarI 622 GAAGAG CTCTTC(1/4)
EcoRV 658 GATATC GAT^ATC
EcoT22I 604 ATGCAT ATGCA^T
FokI 87 CATCC GGATG(9/13)
HapII 18 CCGG C^CGG
HindIII 377 AAGCTT A^AGCTT
HpaII 18 CCGG C^CGG
HphI 616 TCACC GGTGA(8/7)
KpnI 20 GGTACC GGTAC^C
Ksp632I 622 GAAGAG CTCTTC(1/4)
MaeII 629 ACGT A^CGT
MboII 622 GAAGA GAAGA(8/7)
MflI 235 GGATCT [GA]^GATC[CT]
MslI 87 CATCCGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 548 CCGCTG C[AC]G^C[GT]G
MspI 18 CCGG C^CGG
MwoI 550 GCTGCAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 17 CCCGG CC^[GC]GG
NlaIII 199 CATG CATG^
NsiI 604 ATGCAT ATGCA^T
PvuI 35 CGATCG CGAT^CG
ScrFI 17 CCCGG CC^[ACGT]GG
SfaNI 603 GATGC GCATC(5/9)
SnaBI 628 TACGTA TAC^GTA
SspBI 195 TGTACA T^GTACA
XhoII 235 GGATCT [GA]^GATC[CT]
XmnI 438 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 112 GTCTAC GT^[AC][GT]AC
420 GTATAC
AciI 74 CCGC CCGC(-3/-1)
548 CCGC
BmyI 82 GTGCAC G[AGT]GC[ACT]^C
557 GAGCCC
BsaJI 16 CCCCGG C^C[ACGT][ACGT]GG
231 CCTCGG
BsiYI 11 CCGACCCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
648 CCTCTACTTGG
BslI 11 CCGACCCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
648 CCTCTACTTGG
Bsp1286I 82 GTGCAC G[AGT]GC[ACT]^C
557 GAGCCC
DpnII 36 GATC ^GATC
236 GATC
HaeIII 69 GGCC GG^CC
96 GGCC
MboI 36 GATC ^GATC
236 GATC
NdeII 36 GATC ^GATC
236 GATC
NlaIV 20 GGTACC GG[ACGT]^[ACGT]CC
556 GGAGCC
PleI 178 GAGTC GAGTC(4/5)
204 GAGTC
PstI 130 CTGCAG CTGCA^G
551 CTGCAG
Sau3AI 36 GATC ^GATC
236 GATC
TaqI 34 TCGA T^CGA
38 TCGA
TfiI 500 GATTC G^A[AT]TC
575 GATTC
Tsp45I 151 GTGAC ^GT[GC]AC
615 GTCAC
Tsp509I 216 AATT ^AATT
452 AATT
TspRI 149 CAGTG CA[GC]TG[ACGT][ACGT]^
599 CACTG
TthHB8I 34 TCGA T^CGA
38 TCGA
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AfaI AluI BbvI DdeI Fnu4HI
HinfI ItaI MaeIII MnlI MseI
RsaI SfcI Tru9I TseI