##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BcgI        BciVI       BcnI        BfrI        BglI        
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FseI        FspI        HaeII       
HapII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MspI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
TaqI        Tru9I       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcsI           31  GAATTT               [GA]^AATT[CT]
ApaI          511  GGGCCC               GGGCC^C
ApoI           31  GAATTT               [GA]^AATT[CT]
Asp700I        31  GAATTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII         511  GGGCCC               G[GA]GC[CT]^C
BbvCI         903  GCTGAGG              CCTCAGC(-5/-2)
BclI          668  TGATCA               T^GATCA
BglII         302  AGATCT               A^GATCT
BmyI          511  GGGCCC               G[AGT]GC[ACT]^C
BsmAI         621  GTCTC                GTCTC(1/5)
BsmFI         544  GTCCC                GGGAC(10/14)
BsmI          923  GCATTC               GAATGC(1/-1)
Bsp1286I      511  GGGCCC               G[AGT]GC[ACT]^C
BspHI         407  TCATGA               T^CATGA
BstXI         515  CCAATGAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         302  AGATCT               [GA]^GATC[CT]
CfoI          393  GCGC                 GCG^C
EarI           79  CTCTTC               CTCTTC(1/4)
FbaI          668  TGATCA               T^GATCA
HhaI          393  GCGC                 GCG^C
HinP1I        393  GCGC                 G^CGC
HinfI          16  GATTC                G^A[ACGT]TC
Ksp632I        79  CTCTTC               CTCTTC(1/4)
MflI          302  AGATCT               [GA]^GATC[CT]
MslI          576  CACCTATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        496  CAGCTG               C[AC]G^C[GT]G
PshAI         735  GACAGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         496  CAGCTG               CAG^CTG
RcaI          407  TCATGA               T^CATGA
SpeI          486  ACTAGT               A^CTAGT
StuI          781  AGGCCT               AGG^CCT
TfiI           16  GATTC                G^A[AT]TC
Tsp45I         25  GTGAC                ^GT[GC]AC
Tsp509I        32  AATT                 ^AATT
XhoII         302  AGATCT               [GA]^GATC[CT]
XmnI           31  GAATTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BbsI          885  GAAGAC               GAAGAC(2/6)
              709  GTCTTC              
BfaI          322  CTAG                 C^TAG
              487  CTAG                
BpuAI         885  GAAGAC               GAAGAC(2/6)
              709  GTCTTC              
BsaJI         117  CCTGGG               C^C[ACGT][ACGT]GG
              693  CCAGGG              
BspMI         161  ACCTGC               ACCTGC(4/8)
              477  GCAGGT              
BsrDI         105  CATTGC               GCAATG(2/0)
              929  CATTGC              
BsrI          522  ACTGG                ACTGG(1/-1)
              329  CCAGT               
EcoNI         258  CCTACAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              359  CCTATGACAGG         
MaeI          322  CTAG                 C^TAG
              487  CTAG                
NlaIV         325  GGAGCC               GG[ACGT]^[ACGT]CC
              511  GGGCCC              
PstI           67  CTGCAG               CTGCA^G
              789  CTGCAG              
SfaNI         290  GATGC                GCATC(5/9)
              275  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AluI        BbvI        BsiYI       BslI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HaeIII      
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MvaI        NdeII       NlaIII      Sau3AI      
Sau96I      ScrFI       SfcI        TseI        TspRI