Restriction map for
pF1KB7439
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# NO CUT ENZYMES ( 176 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BmyI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI StyI SwaI
TaqI TfiI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 259 GGCGTC G[GA]^CG[CT]C
Asp700I 96 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 231 TGCGCA TGC^GCA
BcnI 335 CCGGG CC^[GC]GG
BlpI 63 GCTTAGC GC^T[ACGT]AGC
BpmI 300 CTGGAG CTGGAG(16/14)
Bpu1102I 63 GCTTAGC GC^T[ACGT]AGC
BsaHI 259 GGCGTC G[GA]^CG[CT]C
BseRI 203 GAGGAG GAGGAG(10/8)
BsmI 395 GCATTC GAATGC(1/-1)
BspMI 42 GCAGGT ACCTGC(4/8)
BstAPI 277 GCACTGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 235 CATCC GGATG(2/0)
CelII 63 GCTTAGC GC^T[ACGT]AGC
CfoI 232 GCGC GCG^C
Cfr13I 50 GGCCC G^G[ACGT]CC
DdeI 64 CTTAG C^T[ACGT]AG
EcoNI 177 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 100 GAATTC G^AATTC
FokI 235 CATCC GGATG(9/13)
FspI 231 TGCGCA TGC^GCA
HaeIII 50 GGCC GG^CC
HhaI 232 GCGC GCG^C
Hin1I 259 GGCGTC G[GA]^CG[CT]C
HinP1I 232 GCGC G^CGC
HinfI 343 GACTC G^A[ACGT]TC
HphI 45 GGTGA GGTGA(8/7)
MseI 427 TTAA T^TAA
MspA1I 227 CAGCTG C[AC]G^C[GT]G
NciI 335 CCGGG CC^[GC]GG
NlaIV 327 GGAACC GG[ACGT]^[ACGT]CC
PleI 343 GACTC GAGTC(4/5)
PstI 322 CTGCAG CTGCA^G
PvuII 227 CAGCTG CAG^CTG
Sau96I 50 GGCCC G^G[ACGT]CC
SbfI 321 CCTGCAGG CCTGCA^GG
SfcI 322 CTGCAG C^T[GA][CT]AG
SmlI 188 CTCAAG C^T[CT][GA]AG
Sse8387I 321 CCTGCAGG CCTGCA^GG
Tru9I 427 TTAA T^TAA
XmnI 96 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 291 CCGC CCGC(-3/-1)
256 GCGG
AcsI 100 GAATTC [GA]^AATT[CT]
221 GAATTT
ApoI 100 GAATTC [GA]^AATT[CT]
221 GAATTT
BbvI 316 GCAGC GCAGC(8/12)
229 GCTGC
BsgI 249 CTGCAC GTGCAG(16/14)
275 CTGCAC
BsiYI 11 CCCGATACGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
177 CCTACCCCAGG
BslI 11 CCCGATACGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
177 CCTACCCCAGG
BstNI 183 CCAGG CC^[AT]GG
295 CCTGG
DraIII 278 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
414 CACGAAGTG
EarI 124 GAAGAG CTCTTC(1/4)
142 GAAGAG
EcoRII 183 CCAGG ^CC[AT]GG
295 CCTGG
FauI 290 CCCGC CCCGC(4/6)
256 GCGGG
Fnu4HI 229 GCTGC GC^[ACGT]GC
316 GCAGC
HgaI 260 GCGTC GACGC(5/10)
286 GCGTC
ItaI 229 GCTGC GC^[ACGT]GC
316 GCAGC
Ksp632I 124 GAAGAG CTCTTC(1/4)
142 GAAGAG
MvaI 183 CCAGG CC^[AT]GG
295 CCTGG
SfaNI 34 GATGC GCATC(5/9)
234 GCATC
TseI 229 GCTGC G^C[AT]GC
316 GCAGC
Tsp509I 101 AATT ^AATT
222 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI AlwI BsaJI BsmFI BssKI
BstYI Cac8I DpnII HapII HpaII
MboI MboII MflI MnlI MspI
MwoI NdeII Sau3AI ScrFI TspRI
XhoII