Restriction map for
pF1KB7443
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfaI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiHKAI
BsmI Bsp1407I BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII CpoI
DdeI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI HphI KasI KpnI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tsp509I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 658 GACGTC GACGT^C
AccII 388 CGCG CG^CG
AcyI 658 GACGTC G[GA]^CG[CT]C
AfaI 155 GTAC GT^AC
AflII 30 CTTAAG C^TTAAG
AlwI 498 GGATC GGATC(4/5)
AlwNI 628 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 481 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 375 CGACCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 30 CTTAAG C^TTAAG
BpmI 170 CTCCAG CTGGAG(16/14)
BsaHI 658 GACGTC G[GA]^CG[CT]C
BsiEI 534 CGACCG CG[GA][CT]^CG
BsiWI 154 CGTACG C^GTACG
BsmAI 357 GAGAC GTCTC(1/5)
BsmBI 357 GAGACG CGTCTC(1/5)
BspDI 88 ATCGAT AT^CGAT
BsrI 719 CCAGT ACTGG(1/-1)
BstUI 388 CGCG CG^CG
CfoI 573 GCGC GCG^C
ClaI 88 ATCGAT AT^CGAT
DraIII 59 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 158 CGGCCA [CT]^GGCC[GA]
EarI 318 GAAGAG CTCTTC(1/4)
EcoT14I 439 CCAAGG C^C[AT][AT]GG
HaeII 572 AGCGCC [GA]GCGC^[CT]
HgaI 774 GCGTC GACGC(5/10)
HhaI 573 GCGC GCG^C
Hin1I 658 GACGTC G[GA]^CG[CT]C
HinP1I 573 GCGC G^CGC
Ksp632I 318 GAAGAG CTCTTC(1/4)
KspI 387 CCGCGG CCGC^GG
MaeII 659 ACGT A^CGT
MaeIII 868 GTGAC ^GT[ACGT]AC
MboII 318 GAAGA GAAGA(8/7)
MseI 31 TTAA T^TAA
MvnI 388 CGCG CG^CG
MwoI 363 GCGGCACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 455 GCCGGC GCC^GGC
NgoMIV 455 GCCGGC G^CCGGC
NspI 524 GCATGC [GA]CATG^[CT]
PflFI 481 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 246 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 281 CTGCAG CTGCA^G
RsaI 155 GTAC GT^AC
SacII 387 CCGCGG CCGC^GG
SfcI 281 CTGCAG C^T[GA][CT]AG
SmaI 828 CCCGGG CCC^GGG
SmlI 30 CTTAAG C^T[CT][GA]AG
SphI 524 GCATGC GCATG^C
StyI 439 CCAAGG C^C[AT][AT]GG
TaqI 89 TCGA T^CGA
Tru9I 31 TTAA T^TAA
Tsp45I 868 GTGAC ^GT[GC]AC
Tth111I 481 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 89 TCGA T^CGA
Van91I 246 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 671 CCACACCCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 828 CCCGGG C^CCGGG
XmaI 828 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 557 AGCT AG^CT
815 AGCT
AvaI 622 CCCGAG C^[CT]CG[GA]G
828 CCCGGG
BanI 365 GGCACC G^G[CT][GA]CC
391 GGCACC
BanII 14 GGGCTC G[GA]GC[CT]^C
214 GAGCCC
BmyI 14 GGGCTC G[AGT]GC[ACT]^C
214 GAGCCC
BsgI 577 CTGCAC GTGCAG(16/14)
817 CTGCAC
BsoBI 622 CCCGAG C^[CT]CG[GA]G
828 CCCGGG
Bsp1286I 14 GGGCTC G[AGT]GC[ACT]^C
214 GAGCCC
BsrBI 237 CCGCTC CCGCTC(-3/-3)
607 GAGCGG
BsrFI 114 GCCGGT [GA]^CCGG[CT]
455 GCCGGC
Cfr10I 114 GCCGGT [GA]^CCGG[CT]
455 GCCGGC
DpnII 122 GATC ^GATC
499 GATC
HinfI 168 GACTC G^A[ACGT]TC
277 GAGTC
MboI 122 GATC ^GATC
499 GATC
NdeII 122 GATC ^GATC
499 GATC
PleI 168 GACTC GAGTC(4/5)
277 GAGTC
PpuMI 485 GGGTCCT [GA]G^G[AT]CC[CT]
587 AGGACCC
PvuII 556 CAGCTG CAG^CTG
814 CAGCTG
Sau3AI 122 GATC ^GATC
499 GATC
SfaNI 705 GATGC GCATC(5/9)
539 GCATC
TspRI 61 CACTG CA[GC]TG[ACGT][ACGT]^
720 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AvaII BbvI BcnI BsaJI
BsiYI BslI BsmFI BspMI BssKI
BstF5I BstNI Cac8I Cfr13I DraII
DsaI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII ItaI
MnlI MslI MspA1I MspI MvaI
NciI NlaIII NlaIV Sau96I ScrFI
TseI