Restriction map for
pF1KB7445
##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AvaI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HapII Hin1I HindIII
HpaI HpaII KasI KpnI KspI
MfeI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 517 GTCTAC GT^[AC][GT]AC
AcsI 643 AAATTT [GA]^AATT[CT]
ApaI 264 GGGCCC GGGCC^C
ApoI 643 AAATTT [GA]^AATT[CT]
AseI 534 ATTAAT AT^TAAT
AsnI 534 ATTAAT AT^TAAT
AspI 362 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 264 GGGCCC G[GA]GC[CT]^C
BbvCI 7 GCTGAGG CCTCAGC(-5/-2)
BglI 936 GCCACAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 192 AGATCT A^GATCT
BmyI 264 GGGCCC G[AGT]GC[ACT]^C
BsaBI 469 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 140 GAGACC GGTCTC(1/5)
Bsp1286I 264 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 615 TGTACA T^GTACA
BsrBI 239 GAGCGG CCGCTC(-3/-3)
BsrGI 615 TGTACA T^GTACA
BstAPI 74 GCAAGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 155 GCGC GCG^C
DraI 669 TTTAAA TTT^AAA
EcoNI 98 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 169 CCAAGG C^C[AT][AT]GG
HaeII 154 AGCGCC [GA]GCGC^[CT]
HgaI 1279 GCGTC GACGC(5/10)
HhaI 155 GCGC GCG^C
HinP1I 155 GCGC G^CGC
HincII 368 GTCAAC GT[CT]^[GA]AC
HindII 368 GTCAAC GT[CT]^[GA]AC
MaeIII 979 GTGAC ^GT[ACGT]AC
MamI 469 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 249 CATCGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 1366 GCTAGC G^CTAGC
PflFI 362 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 981 GACTC GAGTC(4/5)
PshBI 534 ATTAAT AT^TAAT
PstI 102 CTGCAG CTGCA^G
SapI 931 GAAGAGC GCTCTTC(1/4)
SfcI 102 CTGCAG C^T[GA][CT]AG
SspBI 615 TGTACA T^GTACA
SspI 760 AATATT AAT^ATT
StuI 1186 AGGCCT AGG^CCT
StyI 169 CCAAGG C^C[AT][AT]GG
SwaI 668 ATTTAAAT ATTT^AAAT
TaqI 186 TCGA T^CGA
Tsp45I 979 GTGAC ^GT[GC]AC
Tth111I 362 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 186 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 113 GCGG CCGC(-3/-1)
241 GCGG
BalI 539 TGGCCA TGG^CCA
1264 TGGCCA
BbsI 347 GTCTTC GAAGAC(2/6)
743 GTCTTC
BpuAI 347 GTCTTC GAAGAC(2/6)
743 GTCTTC
BsaJI 169 CCAAGG C^C[ACGT][ACGT]GG
267 CCCTGG
BsiYI 98 CCTGCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
234 CCCCAGAGCGG
BslI 98 CCTGCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
234 CCCCAGAGCGG
BsmAI 140 GAGAC GTCTC(1/5)
1315 GAGAC
BsmFI 284 GGGAC GGGAC(10/14)
232 GTCCC
BsrDI 1325 CATTGC GCAATG(2/0)
1097 GCAATG
BstF5I 432 GGATG GGATG(2/0)
1386 GGATG
BstYI 192 AGATCT [GA]^GATC[CT]
243 GGATCT
Cfr13I 264 GGGCC G^G[ACGT]CC
265 GGCCC
DdeI 8 CTGAG C^T[ACGT]AG
259 CTCAG
DraII 263 GGGGCCC [GA]G^G[ACGT]CC[CT]
264 GGGCCCT
EaeI 539 TGGCCA [CT]^GGCC[GA]
1264 TGGCCA
EcoO109I 263 GGGGCCC [GA]G^G[ACGT]CC[CT]
264 GGGCCCT
FokI 432 GGATG GGATG(9/13)
1386 GGATG
MaeII 179 ACGT A^CGT
717 ACGT
MflI 192 AGATCT [GA]^GATC[CT]
243 GGATCT
MluNI 539 TGGCCA TGG^CCA
1264 TGGCCA
MscI 539 TGGCCA TGG^CCA
1264 TGGCCA
Sau96I 264 GGGCC G^G[ACGT]CC
265 GGCCC
SfaNI 870 GATGC GCATC(5/9)
1387 GATGC
SmlI 776 CTCAAG C^T[CT][GA]AG
983 CTCAAG
TfiI 574 GAATC G^A[AT]TC
1360 GATTC
XhoII 192 AGATCT [GA]^GATC[CT]
243 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI AlwI BbvI BfaI
BssKI BstNI Cac8I DpnII EarI
EcoRII Fnu4HI HaeIII HinfI HphI
ItaI Ksp632I MaeI MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI ScrFI
Tru9I TseI Tsp509I TspRI