Restriction map for
pF1KB7446
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspDI BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MluNI MscI MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
TaqI TspRI Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 179 TCCGGA T^CCGGA
AciI 354 CCGC CCGC(-3/-1)
AcsI 322 GAATTT [GA]^AATT[CT]
AfaI 648 GTAC GT^AC
AflIII 644 ACATGT A^C[GA][CT]GT
AlwI 49 GATCC GGATC(4/5)
ApoI 322 GAATTT [GA]^AATT[CT]
Asp700I 846 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvI 283 GCAGC GCAGC(8/12)
BclI 513 TGATCA T^GATCA
BfaI 574 CTAG C^TAG
BpmI 481 CTCCAG CTGGAG(16/14)
BsaAI 90 CACGTA [CT]AC^GT[GA]
BsaJI 33 CCTGGG C^C[ACGT][ACGT]GG
BsaWI 179 TCCGGA [AT]^CCGG[AT]
BseAI 179 TCCGGA T^CCGGA
BseRI 478 CTCCTC GAGGAG(10/8)
Bsp1407I 647 TGTACA T^GTACA
BspEI 179 TCCGGA T^CCGGA
BspHI 395 TCATGA T^CATGA
BspLU11I 644 ACATGT A^CATGT
BsrGI 647 TGTACA T^GTACA
BstXI 227 CCAGATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 371 GCAAGC GC[ACGT]^[ACGT]GC
EcoT22I 219 ATGCAT ATGCA^T
FbaI 513 TGATCA T^GATCA
Fnu4HI 283 GCAGC GC^[ACGT]GC
HaeIII 37 GGCC GG^CC
HapII 180 CCGG C^CGG
HpaII 180 CCGG C^CGG
ItaI 283 GCAGC GC^[ACGT]GC
MaeI 574 CTAG C^TAG
MroI 179 TCCGGA T^CCGGA
MslI 309 CATGGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 484 CAGCTG C[AC]G^C[GT]G
MspI 180 CCGG C^CGG
NlaIV 569 GGCTCC GG[ACGT]^[ACGT]CC
NsiI 219 ATGCAT ATGCA^T
PflMI 6 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 145 GACTC GAGTC(4/5)
PstI 154 CTGCAG CTGCA^G
PvuII 484 CAGCTG CAG^CTG
RcaI 395 TCATGA T^CATGA
RsaI 648 GTAC GT^AC
SfcI 154 CTGCAG C^T[GA][CT]AG
SspBI 647 TGTACA T^GTACA
TfiI 671 GAATC G^A[AT]TC
TseI 283 GCAGC G^C[AT]GC
Van91I 6 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 846 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AvaII 659 GGACC G^G[AT]CC
903 GGTCC
BsgI 411 CTGCAC GTGCAG(16/14)
281 GTGCAG
BsiYI 6 CCACACAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
480 CCTCCAGCTGG
BslI 6 CCACACAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
480 CCTCCAGCTGG
BsrI 109 ACTGG ACTGG(1/-1)
683 CCAGT
BstF5I 222 CATCC GGATG(2/0)
389 CATCC
DpnII 49 GATC ^GATC
514 GATC
FokI 222 CATCC GGATG(9/13)
389 CATCC
HinfI 145 GACTC G^A[ACGT]TC
671 GAATC
MaeII 91 ACGT A^CGT
506 ACGT
MboI 49 GATC ^GATC
514 GATC
MseI 100 TTAA T^TAA
334 TTAA
NdeII 49 GATC ^GATC
514 GATC
NspI 523 GCATGT [GA]CATG^[CT]
644 ACATGT
Sau3AI 49 GATC ^GATC
514 GATC
SfaNI 221 GCATC GCATC(5/9)
793 GCATC
Tru9I 100 TTAA T^TAA
334 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BssKI BstNI Cfr13I DdeI
EcoRII HphI MaeIII MboII MnlI
MvaI NlaIII Sau96I ScrFI Tsp45I
Tsp509I