Restriction map for
pF1KB7447
##################################
# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MflI MluI MunI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SpeI SphI Sse8387I StyI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 441 CGCG CG^CG
AccIII 365 TCCGGA T^CCGGA
AciI 490 GCGG CCGC(-3/-1)
AcsI 768 AAATTC [GA]^AATT[CT]
ApoI 768 AAATTC [GA]^AATT[CT]
BalI 532 TGGCCA TGG^CCA
BbsI 586 GTCTTC GAAGAC(2/6)
BpuAI 586 GTCTTC GAAGAC(2/6)
BsaI 346 GAGACC GGTCTC(1/5)
BsaJI 139 CCCAGG C^C[ACGT][ACGT]GG
BseAI 365 TCCGGA T^CCGGA
BsgI 243 CTGCAC GTGCAG(16/14)
BsmAI 346 GAGAC GTCTC(1/5)
Bsp1407I 660 TGTACA T^GTACA
BspEI 365 TCCGGA T^CCGGA
BsrDI 509 GCAATG GCAATG(2/0)
BsrGI 660 TGTACA T^GTACA
BstUI 441 CGCG CG^CG
BstXI 683 CCAAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 793 GGCCC G^G[ACGT]CC
DraI 30 TTTAAA TTT^AAA
DrdI 514 GACAATGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 532 TGGCCA [CT]^GGCC[GA]
EarI 214 GAAGAG CTCTTC(1/4)
EcoRV 54 GATATC GAT^ATC
HinfI 191 GAATC G^A[ACGT]TC
HphI 446 TCACC GGTGA(8/7)
Ksp632I 214 GAAGAG CTCTTC(1/4)
MluNI 532 TGGCCA TGG^CCA
MroI 365 TCCGGA T^CCGGA
MscI 532 TGGCCA TGG^CCA
MslI 447 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 488 CAGCGG C[AC]G^C[GT]G
MvnI 441 CGCG CG^CG
NruI 440 TCGCGA TCG^CGA
Sau96I 793 GGCCC G^G[ACGT]CC
SnaBI 595 TACGTA TAC^GTA
SspBI 660 TGTACA T^GTACA
SspI 411 AATATT AAT^ATT
StuI 638 AGGCCT AGG^CCT
TfiI 191 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AlwNI 189 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
674 CAGACACTG
Asp700I 408 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
696 GAAGAACTTC
AspHI 297 GAGCTC G[AT]GC[AT]^C
319 GAGCTC
BpmI 106 CTCCAG CTGGAG(16/14)
691 CTGGAG
BsaAI 433 CACGTA [CT]AC^GT[GA]
595 TACGTA
BsaWI 365 TCCGGA [AT]^CCGG[AT]
482 ACCGGA
BsiHKAI 297 GAGCTC G[AT]GC[AT]^C
319 GAGCTC
BsmFI 130 GGGAC GGGAC(10/14)
250 GGGAC
BssKI 108 CCAGG ^CC[ACGT]GG
140 CCAGG
BstF5I 170 CATCC GGATG(2/0)
210 GGATG
BstNI 108 CCAGG CC^[AT]GG
140 CCAGG
Cac8I 112 GCAAGC GC[ACGT]^[ACGT]GC
629 GCAGGC
CfoI 258 GCGC GCG^C
627 GCGC
DdeI 176 CTCAG C^T[ACGT]AG
300 CTCAG
DpnII 444 GATC ^GATC
654 GATC
EcoRII 108 CCAGG ^CC[AT]GG
140 CCAGG
FokI 170 CATCC GGATG(9/13)
210 GGATG
HapII 366 CCGG C^CGG
483 CCGG
HhaI 258 GCGC GCG^C
627 GCGC
HinP1I 258 GCGC G^CGC
627 GCGC
HpaII 366 CCGG C^CGG
483 CCGG
MaeIII 197 GTCAC ^GT[ACGT]AC
424 GTCAC
MboI 444 GATC ^GATC
654 GATC
MseI 31 TTAA T^TAA
74 TTAA
MspI 366 CCGG C^CGG
483 CCGG
MvaI 108 CCAGG CC^[AT]GG
140 CCAGG
NdeII 444 GATC ^GATC
654 GATC
NlaIV 143 GGAGCC GG[ACGT]^[ACGT]CC
793 GGCCCC
PstI 186 CTGCAG CTGCA^G
782 CTGCAG
SacI 297 GAGCTC GAGCT^C
319 GAGCTC
Sau3AI 444 GATC ^GATC
654 GATC
ScrFI 108 CCAGG CC^[ACGT]GG
140 CCAGG
SfaNI 82 GATGC GCATC(5/9)
733 GATGC
Tru9I 31 TTAA T^TAA
74 TTAA
Tsp45I 197 GTCAC ^GT[GC]AC
424 GTCAC
XmnI 408 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
696 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AfaI AluI BanII BbvI BmyI
BsiYI BslI Bsp1286I BsrI Fnu4HI
HaeIII ItaI MaeII MboII MnlI
NlaIII RsaI SfcI TseI Tsp509I
TspRI