Restriction map for
pF1KB7450
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeII MfeI MflI
MluI MroI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp509I Tth111I Van91I XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 530 GTCTAC GT^[AC][GT]AC
AflII 381 CTTAAG C^TTAAG
AflIII 712 ACATGT A^C[GA][CT]GT
AlwI 503 GATCC GGATC(4/5)
BalI 270 TGGCCA TGG^CCA
BbsI 742 GTCTTC GAAGAC(2/6)
BclI 147 TGATCA T^GATCA
BfrI 381 CTTAAG C^TTAAG
BpmI 683 CTGGAG CTGGAG(16/14)
BpuAI 742 GTCTTC GAAGAC(2/6)
BsaBI 72 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 686 GAGGAG GAGGAG(10/8)
BsmBI 580 CGTCTC CGTCTC(1/5)
BspLU11I 712 ACATGT A^CATGT
BstXI 30 CCAGCTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 701 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 270 TGGCCA [CT]^GGCC[GA]
EarI 125 CTCTTC CTCTTC(1/4)
EcoO109I 701 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 432 GCGGG CCCGC(4/6)
FbaI 147 TGATCA T^GATCA
HphI 569 TCACC GGTGA(8/7)
Ksp632I 125 CTCTTC CTCTTC(1/4)
MamI 72 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 270 TGGCCA TGG^CCA
MscI 270 TGGCCA TGG^CCA
NheI 552 GCTAGC G^CTAGC
NlaIV 277 GGAGCC GG[ACGT]^[ACGT]CC
PleI 325 GAGTC GAGTC(4/5)
SfaNI 734 GCATC GCATC(5/9)
StuI 293 AGGCCT AGG^CCT
TaqI 501 TCGA T^CGA
TthHB8I 501 TCGA T^CGA
XbaI 928 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AciI 372 CCGC CCGC(-3/-1)
432 GCGG
AfaI 171 GTAC GT^AC
511 GTAC
BanII 4 GGGCTC G[GA]GC[CT]^C
163 GAGCCC
BmyI 4 GGGCTC G[AGT]GC[ACT]^C
163 GAGCCC
BsiYI 45 CCCAGGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
81 CCCATTATTGG
BslI 45 CCCAGGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
81 CCCATTATTGG
BsmAI 581 GTCTC GTCTC(1/5)
718 GTCTC
Bsp1286I 4 GGGCTC G[AGT]GC[ACT]^C
163 GAGCCC
BsrDI 116 GCAATG GCAATG(2/0)
607 GCAATG
Cfr13I 695 GGGCC G^G[ACGT]CC
702 GGCCC
DsaI 273 CCATGG C^C[GA][CT]GG
344 CCATGG
MaeIII 211 GTGAC ^GT[ACGT]AC
751 GTCAC
MseI 382 TTAA T^TAA
875 TTAA
MslI 535 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
813 CATCACAATG
NcoI 273 CCATGG C^CATGG
344 CCATGG
NspI 712 ACATGT [GA]CATG^[CT]
792 GCATGT
RsaI 171 GTAC GT^AC
511 GTAC
Sau96I 695 GGGCC G^G[ACGT]CC
702 GGCCC
SmlI 381 CTTAAG C^T[CT][GA]AG
658 CTCAAG
TfiI 654 GATTC G^A[AT]TC
888 GATTC
Tru9I 382 TTAA T^TAA
875 TTAA
Tsp45I 211 GTGAC ^GT[GC]AC
751 GTCAC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI BbvI BfaI BsaJI BsrI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI ItaI MaeI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII Sau3AI ScrFI SfcI StyI
TseI TspRI