Restriction map for
pF1KB7451
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbrPI BbvCI BciVI
BclI BcnI BfaI BglI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HpaI KpnI
KspI MaeI MamI MfeI MluI
MroI MunI NciI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 978 AAATTC [GA]^AATT[CT]
AflII 1048 CTTAAG C^TTAAG
ApoI 978 AAATTC [GA]^AATT[CT]
AvaI 540 CTCGGG C^[CT]CG[GA]G
BbeI 9 GGCGCC GGCGC^C
BbsI 153 GTCTTC GAAGAC(2/6)
BcgI 285 GCAGTGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1048 CTTAAG C^TTAAG
BlpI 243 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 243 GCTCAGC GC^T[ACGT]AGC
BpuAI 153 GTCTTC GAAGAC(2/6)
BsiEI 197 CGGCCG CG[GA][CT]^CG
BsmBI 999 GAGACG CGTCTC(1/5)
BsoBI 540 CTCGGG C^[CT]CG[GA]G
BspMI 533 ACCTGC ACCTGC(4/8)
BsrBI 1167 CCGCTC CCGCTC(-3/-3)
BssSI 14 CACGAG CACGAG(-5/-1)
BstAPI 465 GCAGTATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 243 GCTCAGC GC^T[ACGT]AGC
DraII 1104 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 669 CCATGG C^C[GA][CT]GG
EagI 197 CGGCCG C^GGCCG
EclXI 197 CGGCCG C^GGCCG
Eco52I 197 CGGCCG C^GGCCG
EcoNI 497 CCTTTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1104 AGGTCCC [GA]G^G[ACGT]CC[CT]
HincII 612 GTCAAC GT[CT]^[GA]AC
HindII 612 GTCAAC GT[CT]^[GA]AC
HindIII 546 AAGCTT A^AGCTT
KasI 9 GGCGCC G^GCGCC
NaeI 202 GCCGGC GCC^GGC
NarI 9 GGCGCC GG^CGCC
NcoI 669 CCATGG C^CATGG
NgoMIV 202 GCCGGC G^CCGGC
NotI 196 GCGGCCGC GC^GGCCGC
NspI 1141 ACATGC [GA]CATG^[CT]
PflMI 744 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1104 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 859 CTGCAG CTGCA^G
SapI 787 GAAGAGC GCTCTTC(1/4)
ScaI 1052 AGTACT AGT^ACT
SfcI 859 CTGCAG C^T[GA][CT]AG
SfoI 9 GGCGCC GGC^GCC
SgrAI 207 CACCGGCG C[GA]^CCGG[CT]G
TfiI 664 GAATC G^A[AT]TC
Van91I 744 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 451 GTAGAC GT^[AC][GT]AC
943 GTCTAC
AccII 129 CGCG CG^CG
189 CGCG
AcyI 9 GGCGCC G[GA]^CG[CT]C
1115 GACGCC
BalI 585 TGGCCA TGG^CCA
672 TGGCCA
BamHI 603 GGATCC G^GATCC
1125 GGATCC
BanI 9 GGCGCC G^G[CT][GA]CC
205 GGCACC
BpmI 238 CTGGAG CTGGAG(16/14)
751 CTGGAG
BsaHI 9 GGCGCC G[GA]^CG[CT]C
1115 GACGCC
BsmAI 999 GAGAC GTCTC(1/5)
1099 GAGAC
BsmFI 132 GTCCC GGGAC(10/14)
1106 GTCCC
BsrFI 202 GCCGGC [GA]^CCGG[CT]
208 ACCGGC
BstUI 129 CGCG CG^CG
189 CGCG
BstYI 603 GGATCC [GA]^GATC[CT]
1125 GGATCC
Cfr10I 202 GCCGGC [GA]^CCGG[CT]
208 ACCGGC
EarI 700 GAAGAG CTCTTC(1/4)
787 GAAGAG
EcoT14I 669 CCATGG C^C[AT][AT]GG
1065 CCTTGG
HaeII 9 GGCGCC [GA]GCGC^[CT]
229 AGCGCC
HgaI 1115 GACGC GACGC(5/10)
130 GCGTC
Hin1I 9 GGCGCC G[GA]^CG[CT]C
1115 GACGCC
HphI 1177 TCACC GGTGA(8/7)
1197 TCACC
Ksp632I 700 GAAGAG CTCTTC(1/4)
787 GAAGAG
MaeII 599 ACGT A^CGT
658 ACGT
MflI 603 GGATCC [GA]^GATC[CT]
1125 GGATCC
MluNI 585 TGGCCA TGG^CCA
672 TGGCCA
MscI 585 TGGCCA TGG^CCA
672 TGGCCA
MslI 456 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
813 CATCCCAGTG
MvnI 129 CGCG CG^CG
189 CGCG
PvuII 893 CAGCTG CAG^CTG
922 CAGCTG
StyI 669 CCATGG C^C[AT][AT]GG
1065 CCTTGG
Tsp45I 410 GTCAC ^GT[GC]AC
1176 GTCAC
XhoII 603 GGATCC [GA]^GATC[CT]
1125 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I