##################################
# NO CUT ENZYMES (  227 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrI        
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MboII       MfeI        MluI        MluNI       
MroI        MscI        MseI        MslI        MspA1I      
MspI        MunI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        ScrFI       SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        StyI        SwaI        
TfiI        Tru9I       Tsp509I     TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AfaI          132  GTAC                 GT^AC
BamHI          93  GGATCC               G^GATCC
BbvI           33  GCAGC                GCAGC(8/12)
BciVI         118  GGATAC               GTATCC(6/5)
BfaI           89  CTAG                 C^TAG
BsaBI          45  GATATATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I      131  TGTACA               T^GTACA
BsrGI         131  TGTACA               T^GTACA
BstYI          93  GGATCC               [GA]^GATC[CT]
DpnII          94  GATC                 ^GATC
Fnu4HI         33  GCAGC                GC^[ACGT]GC
ItaI           33  GCAGC                GC^[ACGT]GC
MaeI           89  CTAG                 C^TAG
MaeII          17  ACGT                 A^CGT
MaeIII         65  GTCAC                ^GT[ACGT]AC
MamI           45  GATATATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI           94  GATC                 ^GATC
MflI           93  GGATCC               [GA]^GATC[CT]
MnlI           25  CCTC                 CCTC(7/6)
NdeII          94  GATC                 ^GATC
NlaIV          93  GGATCC               GG[ACGT]^[ACGT]CC
RsaI          132  GTAC                 GT^AC
Sau3AI         94  GATC                 ^GATC
SspBI         131  TGTACA               T^GTACA
TaqI          171  TCGA                 T^CGA
TseI           33  GCAGC                G^C[AT]GC
Tsp45I         65  GTCAC                ^GT[GC]AC
TthHB8I       171  TCGA                 T^CGA
XhoII          93  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    2 enzymes) #
###################################

AlwI           94  GATCC                GGATC(4/5)
               93  GGATC               
SfaNI         104  GATGC                GCATC(5/9)
              166  GATGC               


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################