##################################
# NO CUT ENZYMES (  181 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanII       BbeI        BbrPI       BcgI        BciVI       
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FseI        FspI        HaeIII      
HapII       HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AcyI            3  GGCGTC               G[GA]^CG[CT]C
AlwI           12  GGATC                GGATC(4/5)
AseI          526  ATTAAT               AT^TAAT
AsnI          526  ATTAAT               AT^TAAT
AvaII         447  GGACC                G^G[AT]CC
BanI          354  GGTGCC               G^G[CT][GA]CC
BbsI          171  GAAGAC               GAAGAC(2/6)
BbvCI         502  GCTGAGG              CCTCAGC(-5/-2)
BclI          390  TGATCA               T^GATCA
BfaI          485  CTAG                 C^TAG
BpmI          362  CTGGAG               CTGGAG(16/14)
BpuAI         171  GAAGAC               GAAGAC(2/6)
BsaHI           3  GGCGTC               G[GA]^CG[CT]C
BsgI          537  CTGCAC               GTGCAG(16/14)
BsmAI          38  GAGAC                GTCTC(1/5)
BspMI         562  GCAGGT               ACCTGC(4/8)
Cac8I         220  GCCAGC               GC[ACGT]^[ACGT]GC
CfoI          468  GCGC                 GCG^C
Cfr13I        447  GGACC                G^G[ACGT]CC
DsaI          547  CCACGG               C^C[GA][CT]GG
EcoNI         498  CCTGGCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          390  TGATCA               T^GATCA
HaeII         467  AGCGCC               [GA]GCGC^[CT]
HhaI          468  GCGC                 GCG^C
Hin1I           3  GGCGTC               G[GA]^CG[CT]C
HinP1I        468  GCGC                 G^CGC
MaeI          485  CTAG                 C^TAG
MaeIII        373  GTGAC                ^GT[ACGT]AC
PshBI         526  ATTAAT               AT^TAAT
PstI          560  CTGCAG               CTGCA^G
Sau96I        447  GGACC                G^G[ACGT]CC
SfcI          560  CTGCAG               C^T[GA][CT]AG
Tsp45I        373  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BsaBI         385  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              508  GATTTCAATC          
BsaJI           8  CCCTGG               C^C[ACGT][ACGT]GG
              547  CCACGG              
BsmFI         436  GGGAC                GGGAC(10/14)
                6  GTCCC               
BspHI         141  TCATGA               T^CATGA
              456  TCATGA              
BstF5I        129  GGATG                GGATG(2/0)
              195  GGATG               
EarI          166  GAAGAG               CTCTTC(1/4)
              615  GAAGAG              
FokI          129  GGATG                GGATG(9/13)
              195  GGATG               
HgaI            4  GCGTC                GACGC(5/10)
              318  GCGTC               
HphI           18  GGTGA                GGTGA(8/7)
              372  GGTGA               
Ksp632I       166  GAAGAG               CTCTTC(1/4)
              615  GAAGAG              
MamI          385  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              508  GATTTCAATC          
NlaIV         157  GGAACC               GG[ACGT]^[ACGT]CC
              354  GGTGCC              
PleI           40  GACTC                GAGTC(4/5)
              365  GAGTC               
RcaI          141  TCATGA               T^CATGA
              456  TCATGA              
TfiI          453  GATTC                G^A[AT]TC
              645  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AfaI        AluI        BbvI        BsiYI       BslI        
BsrI        BssKI       BstNI       DdeI        DpnII       
EcoRII      Fnu4HI      HinfI       ItaI        MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      RsaI        Sau3AI      ScrFI       
Tru9I       TseI        Tsp509I     TspRI