##################################
# NO CUT ENZYMES (  212 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstBI       BstEII      
BstF5I      BstNI       BstPI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DdeI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MspA1I      MspI        
MunI        MvaI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        ScrFI       SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      TspRI       TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccII         156  CGCG                 CG^CG
AciI          157  GCGG                 CCGC(-3/-1)
AcsI           76  AAATTT               [GA]^AATT[CT]
ApoI           76  AAATTT               [GA]^AATT[CT]
AspI           84  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvI           60  GCAGC                GCAGC(8/12)
BsaJI          38  CCTCGG               C^C[ACGT][ACGT]GG
BsiYI          33  CCTCACCTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           33  CCTCACCTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI           7  GAGAC                GTCTC(1/5)
BspHI         164  TCATGA               T^CATGA
BspMI         210  ACCTGC               ACCTGC(4/8)
BsrI          189  ACTGG                ACTGG(1/-1)
BstAPI         60  GCAGCAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         156  CGCG                 CG^CG
BstXI          96  CCAGATCATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII          29  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI            2  TGGCCG               [CT]^GGCC[GA]
EcoO109I       29  AGGACCT              [GA]G^G[ACGT]CC[CT]
Fnu4HI         60  GCAGC                GC^[ACGT]GC
HaeIII          3  GGCC                 GG^CC
HphI           35  TCACC                GGTGA(8/7)
ItaI           60  GCAGC                GC^[ACGT]GC
MboII         184  GAAGA                GAAGA(8/7)
MslI           36  CACCTCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          156  CGCG                 CG^CG
MwoI           60  GCAGCAGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflFI          84  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI          29  AGGACCT              [GA]G^G[AT]CC[CT]
PstI           58  CTGCAG               CTGCA^G
RcaI          164  TCATGA               T^CATGA
SfaNI          66  GATGC                GCATC(5/9)
SfcI           58  CTGCAG               C^T[GA][CT]AG
TseI           60  GCAGC                G^C[AT]GC
Tth111I        84  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AvaII          30  GGACC                G^G[AT]CC
              159  GGACC               
BsgI           25  GTGCAG               GTGCAG(16/14)
               46  GTGCAG              
Cfr13I         30  GGACC                G^G[ACGT]CC
              159  GGACC               
DpnII          99  GATC                 ^GATC
              139  GATC                
MboI           99  GATC                 ^GATC
              139  GATC                
NdeII          99  GATC                 ^GATC
              139  GATC                
NlaIII         87  CATG                 CATG^
              165  CATG                
Sau3AI         99  GATC                 ^GATC
              139  GATC                
Sau96I         30  GGACC                G^G[ACGT]CC
              159  GGACC               
Tsp509I        77  AATT                 ^AATT
              111  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

MnlI