Restriction map for
pF1KB7505
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI HaeII
HaeIII Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI MvnI NarI NciI NcoI
NdeI NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tru9I Tth111I TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 193 GTAGAC GT^[AC][GT]AC
AfaI 160 GTAC GT^AC
AlwNI 16 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 234 GAGCAC G[AT]GC[AT]^C
AviII 23 TGCGCA TGC^GCA
BbsI 147 GTCTTC GAAGAC(2/6)
BclI 116 TGATCA T^GATCA
BfaI 125 CTAG C^TAG
BmyI 234 GAGCAC G[AGT]GC[ACT]^C
BpmI 226 CTGGAG CTGGAG(16/14)
BpuAI 147 GTCTTC GAAGAC(2/6)
BsaJI 224 CCCTGG C^C[ACGT][ACGT]GG
BsgI 64 CTGCAC GTGCAG(16/14)
BsiHKAI 234 GAGCAC G[AT]GC[AT]^C
BsmAI 37 GAGAC GTCTC(1/5)
BsmBI 37 GAGACG CGTCTC(1/5)
Bsp1286I 234 GAGCAC G[AGT]GC[ACT]^C
BsrFI 8 GCCGGC [GA]^CCGG[CT]
BssKI 225 CCTGG ^CC[ACGT]GG
BstF5I 135 GGATG GGATG(2/0)
BstNI 225 CCTGG CC^[AT]GG
Cac8I 8 GCCGGC GC[ACGT]^[ACGT]GC
CfoI 24 GCGC GCG^C
Cfr10I 8 GCCGGC [GA]^CCGG[CT]
DpnII 117 GATC ^GATC
EcoRII 225 CCTGG ^CC[AT]GG
FbaI 116 TGATCA T^GATCA
FokI 135 GGATG GGATG(9/13)
FspI 23 TGCGCA TGC^GCA
HapII 9 CCGG C^CGG
HgaI 39 GACGC GACGC(5/10)
HhaI 24 GCGC GCG^C
HinP1I 24 GCGC G^CGC
HpaII 9 CCGG C^CGG
MaeI 125 CTAG C^TAG
MboI 117 GATC ^GATC
MspA1I 73 CAGCTG C[AC]G^C[GT]G
MspI 9 CCGG C^CGG
MvaI 225 CCTGG CC^[AT]GG
NaeI 8 GCCGGC GCC^GGC
NdeII 117 GATC ^GATC
NgoMIV 8 GCCGGC G^CCGGC
NspI 189 GCATGT [GA]CATG^[CT]
PleI 243 GAGTC GAGTC(4/5)
PvuII 73 CAGCTG CAG^CTG
RsaI 160 GTAC GT^AC
Sau3AI 117 GATC ^GATC
ScrFI 225 CCTGG CC^[ACGT]GG
SmlI 85 CTCAAG C^T[CT][GA]AG
Tsp509I 98 AATT ^AATT
TspRI 156 CACTG CA[GC]TG[ACGT][ACGT]^
XbaI 124 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 13 enzymes) #
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AciI 81 CCGC CCGC(-3/-1)
48 GCGG
AluI 74 AGCT AG^CT
230 AGCT
BlpI 3 GCTGAGC GC^T[ACGT]AGC
231 GCTGAGC
Bpu1102I 3 GCTGAGC GC^T[ACGT]AGC
231 GCTGAGC
BsiYI 9 CCGGCTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
81 CCGCCTCAAGG
BslI 9 CCGGCTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
81 CCGCCTCAAGG
CelII 3 GCTGAGC GC^T[ACGT]AGC
231 GCTGAGC
DdeI 4 CTGAG C^T[ACGT]AG
232 CTGAG
MaeIII 171 GTCAC ^GT[ACGT]AC
250 GTGAC
MwoI 3 GCTGAGCCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
66 GCACGACCAGC
SfaNI 141 GATGC GCATC(5/9)
205 GCATC
TfiI 283 GAATC G^A[AT]TC
289 GATTC
Tsp45I 171 GTCAC ^GT[GC]AC
250 GTGAC
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BbvI BseRI EarI Fnu4HI HinfI
ItaI Ksp632I MboII MnlI NlaIII
TseI