##################################
# NO CUT ENZYMES (  212 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AcsI          238  GAATTC               [GA]^AATT[CT]
AluI           62  AGCT                 AG^CT
AlwNI         195  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          238  GAATTC               [GA]^AATT[CT]
AspHI          61  GAGCTC               G[AT]GC[AT]^C
BbsI           41  GTCTTC               GAAGAC(2/6)
BbvI          136  GCAGC                GCAGC(8/12)
BfaI           55  CTAG                 C^TAG
BpuAI          41  GTCTTC               GAAGAC(2/6)
BsaAI         189  TACGTA               [CT]AC^GT[GA]
BsiHKAI        61  GAGCTC               G[AT]GC[AT]^C
BsmAI          72  GTCTC                GTCTC(1/5)
DdeI          146  CTCAG                C^T[ACGT]AG
DpnII          81  GATC                 ^GATC
EcoRI         238  GAATTC               G^AATTC
Fnu4HI        136  GCAGC                GC^[ACGT]GC
ItaI          136  GCAGC                GC^[ACGT]GC
MaeI           55  CTAG                 C^TAG
MaeII         190  ACGT                 A^CGT
MaeIII        250  GTGAC                ^GT[ACGT]AC
MboI           81  GATC                 ^GATC
MboII          42  TCTTC                GAAGA(8/7)
MseI          263  TTAA                 T^TAA
NdeII          81  GATC                 ^GATC
NlaIV         310  GGCTCC               GG[ACGT]^[ACGT]CC
PleI          164  GAGTC                GAGTC(4/5)
SacI           61  GAGCTC               GAGCT^C
Sau3AI         81  GATC                 ^GATC
SfcI          320  CTACAG               C^T[GA][CT]AG
SnaBI         189  TACGTA               TAC^GTA
TfiI          197  GATTC                G^A[AT]TC
Tru9I         263  TTAA                 T^TAA
TseI          136  GCAGC                G^C[AT]GC
Tsp45I        250  GTGAC                ^GT[GC]AC
TspRI         140  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AfaI          192  GTAC                 GT^AC
              223  GTAC                
BanII          61  GAGCTC               G[GA]GC[CT]^C
              177  GGGCTC              
BmyI           61  GAGCTC               G[AGT]GC[ACT]^C
              177  GGGCTC              
Bsp1286I       61  GAGCTC               G[AGT]GC[ACT]^C
              177  GGGCTC              
BsrI          141  ACTGG                ACTGG(1/-1)
              307  ACTGG               
HinfI         164  GAGTC                G^A[ACGT]TC
              197  GATTC               
HphI          249  GGTGA                GGTGA(8/7)
               83  TCACC               
MnlI          150  GAGG                 CCTC(7/6)
              247  GAGG                
MwoI          127  GCTATAAATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              136  GCAGCACTGGC         
RsaI          192  GTAC                 GT^AC
              223  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

Tsp509I