##################################
# NO CUT ENZYMES (  204 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoT14I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeIII      MamI        MboI        MboII       MflI        
MluI        MroI        MslI        MspA1I      MspI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIV       NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AfaI          234  GTAC                 GT^AC
AflIII        201  ACATGT               A^C[GA][CT]GT
AluI          256  AGCT                 AG^CT
AvaII          37  GGACC                G^G[AT]CC
BalI           75  TGGCCA               TGG^CCA
BbvI          316  GCTGC                GCAGC(8/12)
BsaAI         231  TACGTA               [CT]AC^GT[GA]
BsaJI         144  CCCAGG               C^C[ACGT][ACGT]GG
BseRI          10  GAGGAG               GAGGAG(10/8)
BspLU11I      201  ACATGT               A^CATGT
BsrI          300  ACTGG                ACTGG(1/-1)
BssKI         145  CCAGG                ^CC[ACGT]GG
BstF5I          3  GGATG                GGATG(2/0)
BstNI         145  CCAGG                CC^[AT]GG
DraII          36  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI           75  TGGCCA               [CT]^GGCC[GA]
EcoO109I       36  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI         274  GAATTC               G^AATTC
EcoRII        145  CCAGG                ^CC[AT]GG
EcoRV          63  GATATC               GAT^ATC
Fnu4HI        316  GCTGC                GC^[ACGT]GC
FokI            3  GGATG                GGATG(9/13)
HinfI         267  GATTC                G^A[ACGT]TC
ItaI          316  GCTGC                GC^[ACGT]GC
MaeII         232  ACGT                 A^CGT
MfeI          281  CAATTG               C^AATTG
MluNI          75  TGGCCA               TGG^CCA
MscI           75  TGGCCA               TGG^CCA
MseI          109  TTAA                 T^TAA
MunI          281  CAATTG               C^AATTG
MvaI          145  CCAGG                CC^[AT]GG
PpuMI          36  AGGACCT              [GA]G^G[AT]CC[CT]
RsaI          234  GTAC                 GT^AC
ScrFI         145  CCAGG                CC^[ACGT]GG
SfaNI          43  GATGC                GCATC(5/9)
SnaBI         231  TACGTA               TAC^GTA
TaqI          211  TCGA                 T^CGA
TfiI          267  GATTC                G^A[AT]TC
Tru9I         109  TTAA                 T^TAA
TseI          316  GCTGC                G^C[AT]GC
TspRI         299  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       211  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AcsI           82  GAATTT               [GA]^AATT[CT]
              274  GAATTC              
ApoI           82  GAATTT               [GA]^AATT[CT]
              274  GAATTC              
Cfr13I         37  GGACC                G^G[ACGT]CC
              142  GGCCC               
DdeI          158  CTGAG                C^T[ACGT]AG
              327  CTTAG               
EcoT22I       104  ATGCAT               ATGCA^T
              219  ATGCAT              
HaeIII         76  GGCC                 GG^CC
              142  GGCC                
MnlI           10  GAGG                 CCTC(7/6)
              175  GAGG                
NsiI          104  ATGCAT               ATGCA^T
              219  ATGCAT              
Sau96I         37  GGACC                G^G[ACGT]CC
              142  GGCCC               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

NlaIII      NspI        Tsp509I