Restriction map for
pF1KB7510
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanII BbrPI BbvCI
BbvI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstUI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FauI FbaI Fnu4HI FseI FspI
HgaI HpaI ItaI Ksp632I KspI
MaeI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TseI TspRI Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 277 GGTACC G^GTACC
AcsI 472 AAATTT [GA]^AATT[CT]
AcyI 73 GGCGCC G[GA]^CG[CT]C
AfaI 278 GTAC GT^AC
AlwNI 121 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 472 AAATTT [GA]^AATT[CT]
Asp718I 277 GGTACC G^GTACC
AspHI 97 GTGCTC G[AT]GC[AT]^C
BbeI 73 GGCGCC GGCGC^C
BbsI 34 GAAGAC GAAGAC(2/6)
BcgI 522 GCAAGAATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 97 GTGCTC G[AGT]GC[ACT]^C
BpuAI 34 GAAGAC GAAGAC(2/6)
BsaHI 73 GGCGCC G[GA]^CG[CT]C
BsaI 645 GAGACC GGTCTC(1/5)
BsiHKAI 97 GTGCTC G[AT]GC[AT]^C
BsmI 594 GCATTC GAATGC(1/-1)
Bsp1286I 97 GTGCTC G[AGT]GC[ACT]^C
BspHI 252 TCATGA T^CATGA
BsrDI 625 GCAATG GCAATG(2/0)
BsrFI 88 ACCGGC [GA]^CCGG[CT]
BstAPI 594 GCATTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 609 GGTGACC G^GT[ACGT]ACC
BstPI 609 GGTGACC G^GT[ACGT]ACC
Cfr10I 88 ACCGGC [GA]^CCGG[CT]
DpnII 478 GATC ^GATC
DraII 122 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 614 CCGTGG C^C[GA][CT]GG
EcoNI 114 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 122 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 609 GGTGACC G^GT[ACGT]ACC
EcoT14I 455 CCAAGG C^C[AT][AT]GG
HaeII 73 GGCGCC [GA]GCGC^[CT]
Hin1I 73 GGCGCC G[GA]^CG[CT]C
HincII 319 GTTGAC GT[CT]^[GA]AC
HindII 319 GTTGAC GT[CT]^[GA]AC
KasI 73 GGCGCC G^GCGCC
KpnI 277 GGTACC GGTAC^C
MboI 478 GATC ^GATC
MspA1I 78 CAGCGG C[AC]G^C[GT]G
NarI 73 GGCGCC GG^CGCC
NdeII 478 GATC ^GATC
PflMI 690 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 577 GACTC GAGTC(4/5)
PstI 344 CTGCAG CTGCA^G
RcaI 252 TCATGA T^CATGA
RsaI 278 GTAC GT^AC
Sau3AI 478 GATC ^GATC
SfaNI 45 GATGC GCATC(5/9)
SfcI 344 CTGCAG C^T[GA][CT]AG
SfoI 73 GGCGCC GGC^GCC
SmlI 718 CTCAAG C^T[CT][GA]AG
StuI 496 AGGCCT AGG^CCT
StyI 455 CCAAGG C^C[AT][AT]GG
Van91I 690 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 686 CCATCCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 80 GCGG CCGC(-3/-1)
301 GCGG
BanI 73 GGCGCC G^G[CT][GA]CC
277 GGTACC
BsmAI 645 GAGAC GTCTC(1/5)
653 GAGAC
BstXI 535 CCAGGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
574 CCAGACTCTTGG
Cac8I 76 GCCAGC GC[ACGT]^[ACGT]GC
351 GCTGGC
CfoI 74 GCGC GCG^C
706 GCGC
Cfr13I 123 GGGCC G^G[ACGT]CC
584 GGGCC
EaeI 2 TGGCCG [CT]^GGCC[GA]
150 TGGCCG
HapII 89 CCGG C^CGG
153 CCGG
HhaI 74 GCGC GCG^C
706 GCGC
HinP1I 74 GCGC G^CGC
706 GCGC
HindIII 25 AAGCTT A^AGCTT
179 AAGCTT
HpaII 89 CCGG C^CGG
153 CCGG
MboII 34 GAAGA GAAGA(8/7)
463 GAAGA
MspI 89 CCGG C^CGG
153 CCGG
MwoI 346 GCAGAGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
594 GCATTCTGTGC
Sau96I 123 GGGCC G^G[ACGT]CC
584 GGGCC
TaqI 22 TCGA T^CGA
170 TCGA
Tsp45I 139 GTCAC ^GT[GC]AC
610 GTGAC
TthHB8I 22 TCGA T^CGA
170 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BpmI BsaJI BsiYI BslI
BssKI BstF5I BstNI EcoRII FokI
HaeIII HinfI HphI MaeIII MnlI
MseI MvaI NlaIII NlaIV ScrFI
TfiI Tru9I Tsp509I