##################################
# NO CUT ENZYMES ( 192 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmFI BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrI BssHII
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DdeI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII HphI KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MseI MspA1I
MspI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
Sau96I SbfI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Asp700I 237 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 276 GTGCTC G[AT]GC[AT]^C
AvaI 310 CCCGAG C^[CT]CG[GA]G
BanII 268 GAGCCC G[GA]GC[CT]^C
BbsI 175 GAAGAC GAAGAC(2/6)
BbvI 219 GCAGC GCAGC(8/12)
BpuAI 175 GAAGAC GAAGAC(2/6)
BsgI 160 CTGCAC GTGCAG(16/14)
BsiHKAI 276 GTGCTC G[AT]GC[AT]^C
BsmI 334 GCATTC GAATGC(1/-1)
BsoBI 310 CCCGAG C^[CT]CG[GA]G
Bsp1407I 320 TGTACA T^GTACA
BsrBI 39 CCGCTC CCGCTC(-3/-3)
BsrGI 320 TGTACA T^GTACA
BssSI 265 CACGAG CACGAG(-5/-1)
DpnII 213 GATC ^GATC
EaeI 287 TGGCCG [CT]^GGCC[GA]
FauI 360 CCCGC CCCGC(4/6)
HaeII 325 AGCGCC [GA]GCGC^[CT]
MaeII 247 ACGT A^CGT
MaeIII 23 GTGAC ^GT[ACGT]AC
MboI 213 GATC ^GATC
MslI 246 CACGTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 213 GATC ^GATC
NlaIII 349 CATG CATG^
SapI 123 GAAGAGC GCTCTTC(1/4)
Sau3AI 213 GATC ^GATC
ScaI 239 AGTACT AGT^ACT
SspBI 320 TGTACA T^GTACA
TseI 219 GCAGC G^C[AT]GC
Tsp45I 23 GTGAC ^GT[GC]AC
TspRI 21 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 237 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 195 CGCG CG^CG
362 CGCG
AfaI 240 GTAC GT^AC
321 GTAC
BmyI 268 GAGCCC G[AGT]GC[ACT]^C
276 GTGCTC
BsaJI 51 CCCTGG C^C[ACGT][ACGT]GG
311 CCGAGG
BseRI 44 CTCCTC GAGGAG(10/8)
93 GAGGAG
Bsp1286I 268 GAGCCC G[AGT]GC[ACT]^C
276 GTGCTC
BstUI 195 CGCG CG^CG
362 CGCG
CfoI 326 GCGC GCG^C
363 GCGC
EarI 83 GAAGAG CTCTTC(1/4)
123 GAAGAG
HaeIII 150 GGCC GG^CC
288 GGCC
HhaI 326 GCGC GCG^C
363 GCGC
HinP1I 326 GCGC G^CGC
363 GCGC
HinfI 25 GACTC G^A[ACGT]TC
64 GACTC
Ksp632I 83 GAAGAG CTCTTC(1/4)
123 GAAGAG
MvnI 195 CGCG CG^CG
362 CGCG
PleI 25 GACTC GAGTC(4/5)
64 GACTC
RsaI 240 GTAC GT^AC
321 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI AluI BpmI BsiYI BslI
BssKI BstNI Cac8I EcoRII Fnu4HI
ItaI MboII MnlI MvaI MwoI
ScrFI