################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmFI BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI Sau96I SbfI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Asp700I 237 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 276 GTGCTC G[AT]GC[AT]^C AvaI 310 CCCGAG C^[CT]CG[GA]G BanII 268 GAGCCC G[GA]GC[CT]^C BbsI 175 GAAGAC GAAGAC(2/6) BbvI 219 GCAGC GCAGC(8/12) BpuAI 175 GAAGAC GAAGAC(2/6) BsgI 160 CTGCAC GTGCAG(16/14) BsiHKAI 276 GTGCTC G[AT]GC[AT]^C BsmI 334 GCATTC GAATGC(1/-1) BsoBI 310 CCCGAG C^[CT]CG[GA]G Bsp1407I 320 TGTACA T^GTACA BsrBI 39 CCGCTC CCGCTC(-3/-3) BsrGI 320 TGTACA T^GTACA BssSI 265 CACGAG CACGAG(-5/-1) DpnII 213 GATC ^GATC EaeI 287 TGGCCG [CT]^GGCC[GA] FauI 360 CCCGC CCCGC(4/6) HaeII 325 AGCGCC [GA]GCGC^[CT] MaeII 247 ACGT A^CGT MaeIII 23 GTGAC ^GT[ACGT]AC MboI 213 GATC ^GATC MslI 246 CACGTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 213 GATC ^GATC NlaIII 349 CATG CATG^ SapI 123 GAAGAGC GCTCTTC(1/4) Sau3AI 213 GATC ^GATC ScaI 239 AGTACT AGT^ACT SspBI 320 TGTACA T^GTACA TseI 219 GCAGC G^C[AT]GC Tsp45I 23 GTGAC ^GT[GC]AC TspRI 21 CAGTG CA[GC]TG[ACGT][ACGT]^ XmnI 237 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccII 195 CGCG CG^CG 362 CGCG AfaI 240 GTAC GT^AC 321 GTAC BmyI 268 GAGCCC G[AGT]GC[ACT]^C 276 GTGCTC BsaJI 51 CCCTGG C^C[ACGT][ACGT]GG 311 CCGAGG BseRI 44 CTCCTC GAGGAG(10/8) 93 GAGGAG Bsp1286I 268 GAGCCC G[AGT]GC[ACT]^C 276 GTGCTC BstUI 195 CGCG CG^CG 362 CGCG CfoI 326 GCGC GCG^C 363 GCGC EarI 83 GAAGAG CTCTTC(1/4) 123 GAAGAG HaeIII 150 GGCC GG^CC 288 GGCC HhaI 326 GCGC GCG^C 363 GCGC HinP1I 326 GCGC G^CGC 363 GCGC HinfI 25 GACTC G^A[ACGT]TC 64 GACTC Ksp632I 83 GAAGAG CTCTTC(1/4) 123 GAAGAG MvnI 195 CGCG CG^CG 362 CGCG PleI 25 GACTC GAGTC(4/5) 64 GACTC RsaI 240 GTAC GT^AC 321 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AciI AluI BpmI BsiYI BslI BssKI BstNI Cac8I EcoRII Fnu4HI ItaI MboII MnlI MvaI MwoI ScrFI