Restriction map for
pF1KB7518
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaJI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII HphI KasI KpnI KspI
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
StyI SwaI Tru9I Tsp45I Tth111I
Van91I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AlwNI 644 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 658 CCTGAGG CC^T[ACGT]AGG
BamHI 59 GGATCC G^GATCC
BanI 321 GGCACC G^G[CT][GA]CC
BanII 223 GAGCCC G[GA]GC[CT]^C
BbvI 579 GCAGC GCAGC(8/12)
BmyI 223 GAGCCC G[AGT]GC[ACT]^C
BsaI 670 GAGACC GGTCTC(1/5)
BseRI 661 GAGGAG GAGGAG(10/8)
BsgI 577 GTGCAG GTGCAG(16/14)
BsmFI 261 GTCCC GGGAC(10/14)
Bsp1286I 223 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 684 TGTACA T^GTACA
BspHI 453 TCATGA T^CATGA
BspLU11I 130 ACATGT A^CATGT
BsrDI 300 CATTGC GCAATG(2/0)
BsrGI 684 TGTACA T^GTACA
BsrI 607 CCAGT ACTGG(1/-1)
BstYI 59 GGATCC [GA]^GATC[CT]
Bsu36I 658 CCTGAGG CC^T[ACGT]AGG
Cac8I 213 GCATGC GC[ACGT]^[ACGT]GC
Cfr13I 709 GGCCC G^G[ACGT]CC
DraII 708 AGGCCCC [GA]G^G[ACGT]CC[CT]
EarI 457 GAAGAG CTCTTC(1/4)
Eco81I 658 CCTGAGG CC^T[ACGT]AGG
EcoNI 421 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 708 AGGCCCC [GA]G^G[ACGT]CC[CT]
HaeIII 709 GGCC GG^CC
HincII 46 GTTGAC GT[CT]^[GA]AC
HindII 46 GTTGAC GT[CT]^[GA]AC
Ksp632I 457 GAAGAG CTCTTC(1/4)
MaeII 525 ACGT A^CGT
MflI 59 GGATCC [GA]^GATC[CT]
MspA1I 253 CAGCGG C[AC]G^C[GT]G
PstI 362 CTGCAG CTGCA^G
RcaI 453 TCATGA T^CATGA
SapI 457 GAAGAGC GCTCTTC(1/4)
Sau96I 709 GGCCC G^G[ACGT]CC
SfaNI 532 GATGC GCATC(5/9)
SfcI 362 CTGCAG C^T[GA][CT]AG
SmlI 218 CTCAAG C^T[CT][GA]AG
SphI 213 GCATGC GCATG^C
SspBI 684 TGTACA T^GTACA
TseI 579 GCAGC G^C[AT]GC
XbaI 349 TCTAGA T^CTAGA
XhoII 59 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 201 GTAC GT^AC
685 GTAC
AflIII 130 ACATGT A^C[GA][CT]GT
525 ACGTGT
BfaI 283 CTAG C^TAG
350 CTAG
BpmI 305 CTGGAG CTGGAG(16/14)
590 CTGGAG
BsiYI 325 CCAGACTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
421 CCTTCCAAAGG
BslI 325 CCAGACTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
421 CCTTCCAAAGG
BssKI 87 CCTGG ^CC[ACGT]GG
589 CCTGG
BstNI 87 CCTGG CC^[AT]GG
589 CCTGG
DdeI 550 CTCAG C^T[ACGT]AG
659 CTGAG
EcoRII 87 CCTGG ^CC[AT]GG
589 CCTGG
EcoRV 13 GATATC GAT^ATC
413 GATATC
HinfI 180 GAATC G^A[ACGT]TC
194 GAATC
MaeI 283 CTAG C^TAG
350 CTAG
MboII 457 GAAGA GAAGA(8/7)
496 GAAGA
MvaI 87 CCTGG CC^[AT]GG
589 CCTGG
MwoI 249 GCAACAGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
579 GCAGCTAAAGC
NspI 130 ACATGT [GA]CATG^[CT]
213 GCATGC
RsaI 201 GTAC GT^AC
685 GTAC
ScrFI 87 CCTGG CC^[ACGT]GG
589 CCTGG
TaqI 197 TCGA T^CGA
411 TCGA
TfiI 180 GAATC G^A[AT]TC
194 GAATC
TthHB8I 197 TCGA T^CGA
411 TCGA
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BsmAI BstF5I DpnII Fnu4HI FokI
ItaI MboI MnlI NdeII NlaIII
NlaIV Sau3AI Tsp509I TspRI