Restriction map for
pF1KB7521
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssKI
BssSI Bst1107I BstBI BstEII BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HaeIII HapII Hin1I HincII HindII
HindIII HpaI HpaII HphI KasI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspI MunI MvaI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI ScrFI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 403 GGTACC G^GTACC
AciI 4 GCGG CCGC(-3/-1)
AcsI 36 GAATTC [GA]^AATT[CT]
AfaI 404 GTAC GT^AC
AflIII 304 ACATGT A^C[GA][CT]GT
AlwI 6 GGATC GGATC(4/5)
ApoI 36 GAATTC [GA]^AATT[CT]
Asp718I 403 GGTACC G^GTACC
AvaII 88 GGTCC G^G[AT]CC
BanI 403 GGTACC G^G[CT][GA]CC
BbsI 390 GAAGAC GAAGAC(2/6)
BpuAI 390 GAAGAC GAAGAC(2/6)
BseRI 329 GAGGAG GAGGAG(10/8)
BspLU11I 304 ACATGT A^CATGT
BsrDI 81 GCAATG GCAATG(2/0)
BstAPI 178 GCACTGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 315 GGATG GGATG(2/0)
CfoI 359 GCGC GCG^C
Cfr13I 88 GGTCC G^G[ACGT]CC
EcoRI 36 GAATTC G^AATTC
FokI 315 GGATG GGATG(9/13)
HgaI 28 GACGC GACGC(5/10)
HhaI 359 GCGC GCG^C
HinP1I 359 GCGC G^CGC
KpnI 403 GGTACC GGTAC^C
MaeIII 381 GTCAC ^GT[ACGT]AC
MseI 125 TTAA T^TAA
NheI 262 GCTAGC G^CTAGC
NlaIV 403 GGTACC GG[ACGT]^[ACGT]CC
PleI 427 GACTC GAGTC(4/5)
RsaI 404 GTAC GT^AC
Sau96I 88 GGTCC G^G[ACGT]CC
SspI 109 AATATT AAT^ATT
TaqI 327 TCGA T^CGA
Tru9I 125 TTAA T^TAA
Tsp45I 381 GTCAC ^GT[GC]AC
TthHB8I 327 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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BfaI 263 CTAG C^TAG
277 CTAG
BpmI 310 CTGGAG CTGGAG(16/14)
343 CTGGAG
BsmAI 332 GAGAC GTCTC(1/5)
417 GTCTC
BsrI 230 CCAGT ACTGG(1/-1)
414 CCAGT
DpnII 7 GATC ^GATC
49 GATC
EarI 419 CTCTTC CTCTTC(1/4)
154 GAAGAG
Ksp632I 419 CTCTTC CTCTTC(1/4)
154 GAAGAG
MaeI 263 CTAG C^TAG
277 CTAG
MboI 7 GATC ^GATC
49 GATC
MspA1I 245 CAGCTG C[AC]G^C[GT]G
385 CAGCTG
NdeII 7 GATC ^GATC
49 GATC
NspI 304 ACATGT [GA]CATG^[CT]
335 ACATGC
PvuII 245 CAGCTG CAG^CTG
385 CAGCTG
Sau3AI 7 GATC ^GATC
49 GATC
SfcI 149 CTACAG C^T[GA][CT]AG
242 CTACAG
TfiI 220 GATTC G^A[AT]TC
238 GAATC
Tsp509I 37 AATT ^AATT
123 AATT
TspRI 179 CACTG CA[GC]TG[ACGT][ACGT]^
231 CAGTG
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
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AluI BbvI Cac8I Fnu4HI HinfI
ItaI MboII MnlI MwoI NlaIII
TseI