Restriction map for
pF1KB7529
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI DdeI
DraI DraIII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI KspI
MaeII MamI MfeI MluI MluNI
MscI MseI MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsaI SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tsp509I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccIII 422 TCCGGA T^CCGGA
AcyI 230 GGCGCC G[GA]^CG[CT]C
AlwI 15 GATCC GGATC(4/5)
AspI 430 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 230 GGCGCC G^G[CT][GA]CC
BanII 70 GGGCTC G[GA]GC[CT]^C
BbeI 230 GGCGCC GGCGC^C
BbsI 342 GAAGAC GAAGAC(2/6)
BfaI 202 CTAG C^TAG
BmyI 70 GGGCTC G[AGT]GC[ACT]^C
BpmI 157 CTGGAG CTGGAG(16/14)
BpuAI 342 GAAGAC GAAGAC(2/6)
BsaHI 230 GGCGCC G[GA]^CG[CT]C
BsaWI 422 TCCGGA [AT]^CCGG[AT]
BseAI 422 TCCGGA T^CCGGA
Bsp1286I 70 GGGCTC G[AGT]GC[ACT]^C
BspEI 422 TCCGGA T^CCGGA
BspHI 326 TCATGA T^CATGA
BspMI 412 ACCTGC ACCTGC(4/8)
BsrI 168 ACTGG ACTGG(1/-1)
BstEII 30 GGTGACC G^GT[ACGT]ACC
BstPI 30 GGTGACC G^GT[ACGT]ACC
BstYI 14 AGATCC [GA]^GATC[CT]
Cac8I 37 GCAGGC GC[ACGT]^[ACGT]GC
CpoI 424 CGGACCG CG^G[AT]CCG
DpnII 15 GATC ^GATC
DraII 178 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 441 GACCCCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 211 GAAGAG CTCTTC(1/4)
EcoO109I 178 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 30 GGTGACC G^GT[ACGT]ACC
FauI 303 GCGGG CCCGC(4/6)
HaeII 230 GGCGCC [GA]GCGC^[CT]
Hin1I 230 GGCGCC G[GA]^CG[CT]C
KasI 230 GGCGCC G^GCGCC
Ksp632I 211 GAAGAG CTCTTC(1/4)
MaeI 202 CTAG C^TAG
MaeIII 31 GTGAC ^GT[ACGT]AC
MboI 15 GATC ^GATC
MflI 14 AGATCC [GA]^GATC[CT]
MroI 422 TCCGGA T^CCGGA
MslI 384 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 301 CAGCGG C[AC]G^C[GT]G
NarI 230 GGCGCC GG^CGCC
NdeII 15 GATC ^GATC
PflFI 430 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 49 CCAGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 448 GAGTC GAGTC(4/5)
PstI 298 CTGCAG CTGCA^G
RcaI 326 TCATGA T^CATGA
RsrII 424 CGGACCG CG^G[AT]CCG
Sau3AI 15 GATC ^GATC
SfcI 298 CTGCAG C^T[GA][CT]AG
SfoI 230 GGCGCC GGC^GCC
TfiI 307 GAATC G^A[AT]TC
Tsp45I 31 GTGAC ^GT[GC]AC
Tth111I 430 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 49 CCAGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 14 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 21 GGACC G^G[AT]CC
425 GGACC
BsrBI 313 GAGCGG CCGCTC(-3/-3)
370 GAGCGG
BstF5I 117 CATCC GGATG(2/0)
384 CATCC
CfoI 231 GCGC GCG^C
285 GCGC
FokI 117 CATCC GGATG(9/13)
384 CATCC
HhaI 231 GCGC GCG^C
285 GCGC
HinP1I 231 GCGC G^CGC
285 GCGC
HinfI 307 GAATC G^A[ACGT]TC
448 GAGTC
HphI 30 GGTGA GGTGA(8/7)
162 GGTGA
NlaIII 135 CATG CATG^
327 CATG
PaeR7I 469 CTCGAG C^TCGAG
478 CTCGAG
SmlI 469 CTCGAG C^T[CT][GA]AG
478 CTCGAG
TaqI 470 TCGA T^CGA
479 TCGA
TspRI 432 CAGTG CA[GC]TG[ACGT][ACGT]^
464 CACTG
TthHB8I 470 TCGA T^CGA
479 TCGA
XhoI 469 CTCGAG C^TCGAG
478 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI AvaI BbvI BcnI
BsaJI BseRI BsiYI BslI BsoBI
BssKI BstNI Cfr13I EcoRII Fnu4HI
HaeIII HapII HpaII ItaI MboII
MnlI MspI MvaI MwoI NciI
NlaIV Sau96I ScrFI SfaNI TseI