##################################
# NO CUT ENZYMES ( 166 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AfaI AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BseAI BseRI BsgI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DraII DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MaeIII MamI MboI
MboII MfeI MflI MluI MluNI
MroI MscI MslI MunI NarI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI Sau3AI ScaI SexAI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI SspBI SspI StuI StyI
SwaI Tsp45I Tsp509I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 172 GACGTC GACGT^C
AcyI 172 GACGTC G[GA]^CG[CT]C
AflII 106 CTTAAG C^TTAAG
AscI 282 GGCGCGCC GG^CGCGCC
AspHI 349 GAGCAC G[AT]GC[AT]^C
AviII 38 TGCGCA TGC^GCA
BanI 468 GGTGCC G^G[CT][GA]CC
BfaI 160 CTAG C^TAG
BfrI 106 CTTAAG C^TTAAG
BglI 274 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 172 GACGTC G[GA]^CG[CT]C
BsaWI 465 TCCGGT [AT]^CCGG[AT]
BsiEI 511 CGGCCG CG[GA][CT]^CG
BsiHKAI 349 GAGCAC G[AT]GC[AT]^C
BsmFI 23 GGGAC GGGAC(10/14)
BsrFI 71 GCCGGC [GA]^CCGG[CT]
BsrI 407 CCAGT ACTGG(1/-1)
BstAPI 201 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 71 GCCGGC [GA]^CCGG[CT]
DdeI 97 CTGAG C^T[ACGT]AG
EagI 511 CGGCCG C^GGCCG
EclXI 511 CGGCCG C^GGCCG
Eco52I 511 CGGCCG C^GGCCG
FspI 38 TGCGCA TGC^GCA
Hin1I 172 GACGTC G[GA]^CG[CT]C
HinfI 403 GATTC G^A[ACGT]TC
MaeI 160 CTAG C^TAG
MaeII 173 ACGT A^CGT
MseI 107 TTAA T^TAA
NaeI 71 GCCGGC GCC^GGC
NgoMIV 71 GCCGGC G^CCGGC
NlaIII 221 CATG CATG^
PstI 199 CTGCAG CTGCA^G
SbfI 198 CCTGCAGG CCTGCA^GG
SgrAI 70 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 106 CTTAAG C^T[CT][GA]AG
Sse8387I 198 CCTGCAGG CCTGCA^GG
TaqI 154 TCGA T^CGA
TfiI 403 GATTC G^A[AT]TC
Tru9I 107 TTAA T^TAA
TspRI 408 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 154 TCGA T^CGA
XcmI 371 CCAGCGCCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BcnI 15 CCGGG CC^[GC]GG
135 CCGGG
BsrBI 461 CCGCTC CCGCTC(-3/-3)
365 GAGCGG
BstF5I 117 CATCC GGATG(2/0)
458 CATCC
DsaI 227 CCACGG C^C[GA][CT]GG
514 CCGTGG
EaeI 368 CGGCCA [CT]^GGCC[GA]
511 CGGCCG
FokI 117 CATCC GGATG(9/13)
458 CATCC
MspA1I 55 CCGCTG C[AC]G^C[GT]G
124 CCGCTG
NciI 15 CCGGG CC^[GC]GG
135 CCGGG
NlaIV 321 GGAGCC GG[ACGT]^[ACGT]CC
468 GGTGCC
SfaNI 83 GCATC GCATC(5/9)
457 GCATC
SfcI 199 CTGCAG C^T[GA][CT]AG
267 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AluI AlwNI BanII
BbvI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr13I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI HinP1I HpaII ItaI
MnlI MspI MvaI MvnI MwoI
Sau96I ScrFI TseI