Restriction map for pF1KB7537
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AfaI        AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BseAI       BseRI       BsgI        
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrGI       BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
Eam1105I    EarI        Eco47III    Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        HincII      HindII      
HindIII     HpaI        HphI        KasI        KpnI        
Ksp632I     KspI        MaeIII      MamI        MboI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MunI        NarI        
NcoI        NdeI        NdeII       NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      ScaI        SexAI       SfiI        
SfoI        SfuI        SmaI        SnaBI       SpeI        
SphI        SspBI       SspI        StuI        StyI        
SwaI        Tsp45I      Tsp509I     Tth111I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII         172  GACGTC               GACGT^C
AcyI          172  GACGTC               G[GA]^CG[CT]C
AflII         106  CTTAAG               C^TTAAG
AscI          282  GGCGCGCC             GG^CGCGCC
AspHI         349  GAGCAC               G[AT]GC[AT]^C
AviII          38  TGCGCA               TGC^GCA
BanI          468  GGTGCC               G^G[CT][GA]CC
BfaI          160  CTAG                 C^TAG
BfrI          106  CTTAAG               C^TTAAG
BglI          274  GCCTGTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         172  GACGTC               G[GA]^CG[CT]C
BsaWI         465  TCCGGT               [AT]^CCGG[AT]
BsiEI         511  CGGCCG               CG[GA][CT]^CG
BsiHKAI       349  GAGCAC               G[AT]GC[AT]^C
BsmFI          23  GGGAC                GGGAC(10/14)
BsrFI          71  GCCGGC               [GA]^CCGG[CT]
BsrI          407  CCAGT                ACTGG(1/-1)
BstAPI        201  GCAGGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I         71  GCCGGC               [GA]^CCGG[CT]
DdeI           97  CTGAG                C^T[ACGT]AG
EagI          511  CGGCCG               C^GGCCG
EclXI         511  CGGCCG               C^GGCCG
Eco52I        511  CGGCCG               C^GGCCG
FspI           38  TGCGCA               TGC^GCA
Hin1I         172  GACGTC               G[GA]^CG[CT]C
HinfI         403  GATTC                G^A[ACGT]TC
MaeI          160  CTAG                 C^TAG
MaeII         173  ACGT                 A^CGT
MseI          107  TTAA                 T^TAA
NaeI           71  GCCGGC               GCC^GGC
NgoMIV         71  GCCGGC               G^CCGGC
NlaIII        221  CATG                 CATG^
PstI          199  CTGCAG               CTGCA^G
SbfI          198  CCTGCAGG             CCTGCA^GG
SgrAI          70  CGCCGGCG             C[GA]^CCGG[CT]G
SmlI          106  CTTAAG               C^T[CT][GA]AG
Sse8387I      198  CCTGCAGG             CCTGCA^GG
TaqI          154  TCGA                 T^CGA
TfiI          403  GATTC                G^A[AT]TC
Tru9I         107  TTAA                 T^TAA
TspRI         408  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       154  TCGA                 T^CGA
XcmI          371  CCAGCGCCACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BcnI           15  CCGGG                CC^[GC]GG
              135  CCGGG               
BsrBI         461  CCGCTC               CCGCTC(-3/-3)
              365  GAGCGG              
BstF5I        117  CATCC                GGATG(2/0)
              458  CATCC               
DsaI          227  CCACGG               C^C[GA][CT]GG
              514  CCGTGG              
EaeI          368  CGGCCA               [CT]^GGCC[GA]
              511  CGGCCG              
FokI          117  CATCC                GGATG(9/13)
              458  CATCC               
MspA1I         55  CCGCTG               C[AC]G^C[GT]G
              124  CCGCTG              
NciI           15  CCGGG                CC^[GC]GG
              135  CCGGG               
NlaIV         321  GGAGCC               GG[ACGT]^[ACGT]CC
              468  GGTGCC              
SfaNI          83  GCATC                GCATC(5/9)
              457  GCATC               
SfcI          199  CTGCAG               C^T[GA][CT]AG
              267  CTACAG              


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       BanII       
BbvI        BmyI        BpmI        BsaJI       BsiYI       
BslI        Bsp1286I    BssHII      BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr13I      EcoRII      
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HpaII       ItaI        
MnlI        MspI        MvaI        MvnI        MwoI        
Sau96I      ScrFI       TseI        
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