################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AfaI AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BseRI BsgI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraII DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeIII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MslI MunI NarI NcoI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI ScaI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI Tsp45I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 172 GACGTC GACGT^C AcyI 172 GACGTC G[GA]^CG[CT]C AflII 106 CTTAAG C^TTAAG AscI 282 GGCGCGCC GG^CGCGCC AspHI 349 GAGCAC G[AT]GC[AT]^C AviII 38 TGCGCA TGC^GCA BanI 468 GGTGCC G^G[CT][GA]CC BfaI 160 CTAG C^TAG BfrI 106 CTTAAG C^TTAAG BglI 274 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 172 GACGTC G[GA]^CG[CT]C BsaWI 465 TCCGGT [AT]^CCGG[AT] BsiEI 511 CGGCCG CG[GA][CT]^CG BsiHKAI 349 GAGCAC G[AT]GC[AT]^C BsmFI 23 GGGAC GGGAC(10/14) BsrFI 71 GCCGGC [GA]^CCGG[CT] BsrI 407 CCAGT ACTGG(1/-1) BstAPI 201 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 71 GCCGGC [GA]^CCGG[CT] DdeI 97 CTGAG C^T[ACGT]AG EagI 511 CGGCCG C^GGCCG EclXI 511 CGGCCG C^GGCCG Eco52I 511 CGGCCG C^GGCCG FspI 38 TGCGCA TGC^GCA Hin1I 172 GACGTC G[GA]^CG[CT]C HinfI 403 GATTC G^A[ACGT]TC MaeI 160 CTAG C^TAG MaeII 173 ACGT A^CGT MseI 107 TTAA T^TAA NaeI 71 GCCGGC GCC^GGC NgoMIV 71 GCCGGC G^CCGGC NlaIII 221 CATG CATG^ PstI 199 CTGCAG CTGCA^G SbfI 198 CCTGCAGG CCTGCA^GG SgrAI 70 CGCCGGCG C[GA]^CCGG[CT]G SmlI 106 CTTAAG C^T[CT][GA]AG Sse8387I 198 CCTGCAGG CCTGCA^GG TaqI 154 TCGA T^CGA TfiI 403 GATTC G^A[AT]TC Tru9I 107 TTAA T^TAA TspRI 408 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 154 TCGA T^CGA XcmI 371 CCAGCGCCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BcnI 15 CCGGG CC^[GC]GG 135 CCGGG BsrBI 461 CCGCTC CCGCTC(-3/-3) 365 GAGCGG BstF5I 117 CATCC GGATG(2/0) 458 CATCC DsaI 227 CCACGG C^C[GA][CT]GG 514 CCGTGG EaeI 368 CGGCCA [CT]^GGCC[GA] 511 CGGCCG FokI 117 CATCC GGATG(9/13) 458 CATCC MspA1I 55 CCGCTG C[AC]G^C[GT]G 124 CCGCTG NciI 15 CCGGG CC^[GC]GG 135 CCGGG NlaIV 321 GGAGCC GG[ACGT]^[ACGT]CC 468 GGTGCC SfaNI 83 GCATC GCATC(5/9) 457 GCATC SfcI 199 CTGCAG C^T[GA][CT]AG 267 CTACAG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AccII AciI AluI AlwNI BanII BbvI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MnlI MspI MvaI MvnI MwoI Sau96I ScrFI TseI