Restriction map for
pF1KB7540
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I CelII
ClaI CpoI DpnII DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FbaI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MaeIII MamI MboI
MfeI MflI MluI MroI MseI
MslI MunI MvnI NdeI NdeII
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tru9I Tsp45I
Tsp509I TspRI Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AfaI 342 GTAC GT^AC
AocI 172 CCTCAGG CC^T[ACGT]AGG
BalI 371 TGGCCA TGG^CCA
BbeI 157 GGCGCC GGCGC^C
BglI 131 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 463 CTGCAC GTGCAG(16/14)
BspMI 303 GCAGGT ACCTGC(4/8)
BsrBI 358 GAGCGG CCGCTC(-3/-3)
BsrFI 698 GCCGGC [GA]^CCGG[CT]
BssSI 23 CTCGTG CACGAG(-5/-1)
Bsu36I 172 CCTCAGG CC^T[ACGT]AGG
Cfr10I 698 GCCGGC [GA]^CCGG[CT]
DraIII 20 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 363 GAAGAG CTCTTC(1/4)
Eco81I 172 CCTCAGG CC^T[ACGT]AGG
EcoNI 688 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 767 ATGCAT ATGCA^T
FseI 697 GGCCGGCC GGCCGG^CC
HaeII 157 GGCGCC [GA]GCGC^[CT]
HinfI 420 GAATC G^A[ACGT]TC
HphI 293 TCACC GGTGA(8/7)
KasI 157 GGCGCC G^GCGCC
Ksp632I 363 GAAGAG CTCTTC(1/4)
MaeII 514 ACGT A^CGT
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
NaeI 698 GCCGGC GCC^GGC
NarI 157 GGCGCC GG^CGCC
NgoMIV 698 GCCGGC G^CCGGC
NsiI 767 ATGCAT ATGCA^T
PflMI 19 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 538 CTGCAG CTGCA^G
RsaI 342 GTAC GT^AC
SexAI 409 ACCTGGT A^CC[AT]GGT
SfcI 538 CTGCAG C^T[GA][CT]AG
SfoI 157 GGCGCC GGC^GCC
StuI 106 AGGCCT AGG^CCT
TfiI 420 GAATC G^A[AT]TC
Van91I 19 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BanI 157 GGCGCC G^G[CT][GA]CC
558 GGCACC
BbsI 325 GTCTTC GAAGAC(2/6)
516 GTCTTC
BpmI 42 CTCCAG CTGGAG(16/14)
355 CTGGAG
BpuAI 325 GTCTTC GAAGAC(2/6)
516 GTCTTC
BsaI 482 GGTCTC GGTCTC(1/5)
610 GGTCTC
BseRI 9 CTCCTC GAGGAG(10/8)
12 CTCCTC
BsmAI 483 GTCTC GTCTC(1/5)
611 GTCTC
BsmFI 711 GGGAC GGGAC(10/14)
678 GTCCC
BsrI 62 ACTGG ACTGG(1/-1)
339 CCAGT
BstF5I 152 CATCC GGATG(2/0)
765 GGATG
EaeI 236 TGGCCG [CT]^GGCC[GA]
371 TGGCCA
FokI 152 CATCC GGATG(9/13)
765 GGATG
HgaI 323 GCGTC GACGC(5/10)
676 GCGTC
NcoI 233 CCATGG C^CATGG
296 CCATGG
PpuMI 202 GGGTCCT [GA]G^G[AT]CC[CT]
452 AGGACCC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcyI AluI AlwNI ApaI
AvaI AvaII BanII BbvI BcnI
BmyI BsaHI BsaJI BsiYI BslI
BsoBI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI MboII MnlI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PvuII Sau96I ScrFI SfaNI
SmaI StyI TseI XmaCI XmaI