Restriction map for
pF1KB7543
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglII BlnI BsaAI BsaBI
BsaHI BsaJI BsaWI BseAI BsgI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraII DraIII DrdI DsaI
Eam1105I Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI FseI FspI HaeII Hin1I
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MroI MslI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tsp45I TspRI Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 256 GTCGAC GT^[AC][GT]AC
AcsI 454 GAATTT [GA]^AATT[CT]
AfaI 84 GTAC GT^AC
ApoI 454 GAATTT [GA]^AATT[CT]
AvaII 99 GGACC G^G[AT]CC
BalI 173 TGGCCA TGG^CCA
BanII 392 GAGCCC G[GA]GC[CT]^C
BbsI 531 GAAGAC GAAGAC(2/6)
BbvI 510 GCTGC GCAGC(8/12)
BfaI 242 CTAG C^TAG
BglI 143 GCCGCGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 60 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 60 GCTGAGC GC^T[ACGT]AGC
BpuAI 531 GAAGAC GAAGAC(2/6)
BsaI 473 GGTCTC GGTCTC(1/5)
BsiEI 150 CGGCCG CG[GA][CT]^CG
BsmFI 98 GGGAC GGGAC(10/14)
BsrBI 153 CCGCTC CCGCTC(-3/-3)
BsrFI 101 ACCGGC [GA]^CCGG[CT]
BssKI 120 CCAGG ^CC[ACGT]GG
BssSI 109 CACGAG CACGAG(-5/-1)
BstNI 120 CCAGG CC^[AT]GG
CelII 60 GCTGAGC GC^T[ACGT]AGC
CfoI 6 GCGC GCG^C
Cfr10I 101 ACCGGC [GA]^CCGG[CT]
DpnII 343 GATC ^GATC
EagI 150 CGGCCG C^GGCCG
EarI 430 GAAGAG CTCTTC(1/4)
EclXI 150 CGGCCG C^GGCCG
Eco52I 150 CGGCCG C^GGCCG
EcoRII 120 CCAGG ^CC[AT]GG
EcoT22I 407 ATGCAT ATGCA^T
HgaI 8 GCGTC GACGC(5/10)
HhaI 6 GCGC GCG^C
HinP1I 6 GCGC G^CGC
HincII 256 GTCGAC GT[CT]^[GA]AC
HindII 256 GTCGAC GT[CT]^[GA]AC
HphI 495 GGTGA GGTGA(8/7)
Ksp632I 430 GAAGAG CTCTTC(1/4)
MaeI 242 CTAG C^TAG
MaeIII 520 GTAAC ^GT[ACGT]AC
MboI 343 GATC ^GATC
MluNI 173 TGGCCA TGG^CCA
MscI 173 TGGCCA TGG^CCA
MseI 80 TTAA T^TAA
MvaI 120 CCAGG CC^[AT]GG
NdeII 343 GATC ^GATC
NlaIII 300 CATG CATG^
NsiI 407 ATGCAT ATGCA^T
PflMI 348 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 314 GAGTC GAGTC(4/5)
RsaI 84 GTAC GT^AC
SalI 256 GTCGAC G^TCGAC
Sau3AI 343 GATC ^GATC
ScrFI 120 CCAGG CC^[ACGT]GG
SfaNI 406 GATGC GCATC(5/9)
StuI 422 AGGCCT AGG^CCT
TfiI 303 GATTC G^A[AT]TC
Tru9I 80 TTAA T^TAA
TseI 510 GCTGC G^C[AT]GC
Tsp509I 455 AATT ^AATT
Van91I 348 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 348 CCAAAGACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 508 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
518 CAGTAACTG
BmyI 13 GTGCCC G[AGT]GC[ACT]^C
392 GAGCCC
BpmI 196 CTCCAG CTGGAG(16/14)
373 CTGGAG
BseRI 43 GAGGAG GAGGAG(10/8)
385 GAGGAG
BsiYI 348 CCAAAGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
396 CCAGCAGGAGG
BslI 348 CCAAAGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
396 CCAGCAGGAGG
Bsp1286I 13 GTGCCC G[AGT]GC[ACT]^C
392 GAGCCC
BstF5I 357 GGATG GGATG(2/0)
405 GGATG
Cfr13I 99 GGACC G^G[ACGT]CC
104 GGCCC
DdeI 61 CTGAG C^T[ACGT]AG
261 CTTAG
FokI 357 GGATG GGATG(9/13)
405 GGATG
HapII 102 CCGG C^CGG
208 CCGG
HinfI 303 GATTC G^A[ACGT]TC
314 GAGTC
HpaII 102 CCGG C^CGG
208 CCGG
MspA1I 213 CAGCTG C[AC]G^C[GT]G
508 CAGCTG
MspI 102 CCGG C^CGG
208 CCGG
PvuII 213 CAGCTG CAG^CTG
508 CAGCTG
Sau96I 99 GGACC G^G[ACGT]CC
104 GGCCC
TaqI 35 TCGA T^CGA
257 TCGA
TthHB8I 35 TCGA T^CGA
257 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AccII AciI AluI BsmAI BsrI
BstUI Cac8I EaeI Fnu4HI HaeIII
ItaI MboII MnlI MvnI MwoI
NlaIV