################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspA1I MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AflIII 11 ACACGT A^C[GA][CT]GT AhdI 121 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 527 AGCT AG^CT AlwI 465 GATCC GGATC(4/5) AlwNI 127 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 121 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 327 GAGCAC G[AT]GC[AT]^C AvaI 335 CCCGGG C^[CT]CG[GA]G BalI 93 TGGCCA TGG^CCA BanI 433 GGCACC G^G[CT][GA]CC BbsI 198 GAAGAC GAAGAC(2/6) BglI 363 GCCCATGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 327 GAGCAC G[AGT]GC[ACT]^C BpuAI 198 GAAGAC GAAGAC(2/6) BsaI 448 GAGACC GGTCTC(1/5) BsiHKAI 327 GAGCAC G[AT]GC[AT]^C BsiYI 126 CCAGTCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 126 CCAGTCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 448 GAGAC GTCTC(1/5) BsoBI 335 CCCGGG C^[CT]CG[GA]G Bsp1286I 327 GAGCAC G[AGT]GC[ACT]^C BspHI 230 TCATGA T^CATGA BspMI 273 GCAGGT ACCTGC(4/8) BsrBI 21 GAGCGG CCGCTC(-3/-3) BsrFI 531 GCCGGC [GA]^CCGG[CT] BstBI 265 TTCGAA TT^CGAA BstNI 132 CCTGG CC^[AT]GG BstXI 365 CCATGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 464 AGATCC [GA]^GATC[CT] Cfr10I 531 GCCGGC [GA]^CCGG[CT] DpnII 465 GATC ^GATC EaeI 93 TGGCCA [CT]^GGCC[GA] Eam1105I 121 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRII 132 CCTGG ^CC[AT]GG HaeIII 94 GGCC GG^CC MaeII 13 ACGT A^CGT MaeIII 317 GTAAC ^GT[ACGT]AC MboI 465 GATC ^GATC MflI 464 AGATCC [GA]^GATC[CT] MluNI 93 TGGCCA TGG^CCA MscI 93 TGGCCA TGG^CCA MseI 212 TTAA T^TAA MslI 360 CATGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 132 CCTGG CC^[AT]GG NaeI 531 GCCGGC GCC^GGC NdeII 465 GATC ^GATC NgoMIV 531 GCCGGC G^CCGGC NspI 178 ACATGC [GA]CATG^[CT] NspV 265 TTCGAA TT^CGAA PflMI 126 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 509 GACTC GAGTC(4/5) PstI 185 CTGCAG CTGCA^G RcaI 230 TCATGA T^CATGA Sau3AI 465 GATC ^GATC SfuI 265 TTCGAA TT^CGAA SmaI 335 CCCGGG CCC^GGG Tru9I 212 TTAA T^TAA TspRI 111 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 126 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 194 CCAAGAAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 464 AGATCC [GA]^GATC[CT] XmaCI 335 CCCGGG C^CCGGG XmaI 335 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 23 GCGG CCGC(-3/-1) 369 GCGG AfaI 85 GTAC GT^AC 277 GTAC BbvI 53 GCAGC GCAGC(8/12) 528 GCTGC BcnI 335 CCCGG CC^[GC]GG 336 CCGGG BsaJI 131 CCCTGG C^C[ACGT][ACGT]GG 335 CCCGGG BsrI 126 CCAGT ACTGG(1/-1) 556 CCAGT HapII 336 CCGG C^CGG 532 CCGG HgaI 201 GACGC GACGC(5/10) 297 GCGTC HpaII 336 CCGG C^CGG 532 CCGG MspI 336 CCGG C^CGG 532 CCGG MwoI 47 GCATCGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 363 GCCCATGCGGC NciI 335 CCCGG CC^[GC]GG 336 CCGGG NlaIV 386 GGAACC GG[ACGT]^[ACGT]CC 433 GGCACC RsaI 85 GTAC GT^AC 277 GTAC SfaNI 47 GCATC GCATC(5/9) 482 GCATC SfcI 42 CTACAG C^T[GA][CT]AG 185 CTGCAG TaqI 266 TCGA T^CGA 446 TCGA TfiI 107 GAATC G^A[AT]TC 393 GATTC TseI 53 GCAGC G^C[AT]GC 528 GCTGC TthHB8I 266 TCGA T^CGA 446 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### BfaI BsgI BsmFI BssKI BstF5I Cac8I DdeI Fnu4HI FokI HinfI ItaI MaeI MboII MnlI NlaIII ScrFI Tsp509I