##################################
# NO CUT ENZYMES (  199 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
Eam1105I    EarI        Eco47III    EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        HpaII       HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MamI        MfeI        MflI        
MluI        MroI        MslI        MspA1I      MspI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TseI        Tsp45I      
TspRI       Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AfaI          168  GTAC                 GT^AC
AocI           76  CCTGAGG              CC^T[ACGT]AGG
BbsI          478  GAAGAC               GAAGAC(2/6)
BpmI          472  CTGGAG               CTGGAG(16/14)
BpuAI         478  GAAGAC               GAAGAC(2/6)
BsaI          304  GAGACC               GGTCTC(1/5)
BsiEI           5  CGGCCG               CG[GA][CT]^CG
BspHI         110  TCATGA               T^CATGA
BstXI         293  CCAACTCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I         76  CCTGAGG              CC^T[ACGT]AGG
Cac8I         318  GCTGGC               GC[ACGT]^[ACGT]GC
DpnII         499  GATC                 ^GATC
EagI            5  CGGCCG               C^GGCCG
EclXI           5  CGGCCG               C^GGCCG
Eco52I          5  CGGCCG               C^GGCCG
Eco81I         76  CCTGAGG              CC^T[ACGT]AGG
HindIII       341  AAGCTT               A^AGCTT
MboI          499  GATC                 ^GATC
NdeII         499  GATC                 ^GATC
NlaIV          59  GGCTCC               GG[ACGT]^[ACGT]CC
NspI          269  ACATGC               [GA]CATG^[CT]
PstI          134  CTGCAG               CTGCA^G
RcaI          110  TCATGA               T^CATGA
RsaI          168  GTAC                 GT^AC
Sau3AI        499  GATC                 ^GATC
SfcI          134  CTGCAG               C^T[GA][CT]AG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AcsI          257  AAATTC               [GA]^AATT[CT]
              438  GAATTT              
ApoI          257  AAATTC               [GA]^AATT[CT]
              438  GAATTT              
BalI           38  TGGCCA               TGG^CCA
              527  TGGCCA              
BsmAI         130  GAGAC                GTCTC(1/5)
              304  GAGAC               
DdeI           77  CTGAG                C^T[ACGT]AG
              419  CTCAG               
MluNI          38  TGGCCA               TGG^CCA
              527  TGGCCA              
MscI           38  TGGCCA               TGG^CCA
              527  TGGCCA              
NlaIII        111  CATG                 CATG^
              270  CATG                
PleI          571  GACTC                GAGTC(4/5)
              286  GAGTC               
TaqI          445  TCGA                 T^CGA
              495  TCGA                
TthHB8I       445  TCGA                 T^CGA
              495  TCGA                
XcmI          290  CCACCAACTCAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              308  CCAGATTCTTGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AciI        AluI        BsaJI       BssKI       BstF5I      
BstNI       EaeI        EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       ItaI        MboII       MnlI        
MseI        MvaI        ScrFI       TfiI        Tru9I       
Tsp509I