##################################
# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI DsaI
Eam1105I Eco47III EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AocI 634 CCTGAGG CC^T[ACGT]AGG
AspHI 30 GAGCTC G[AT]GC[AT]^C
AvaI 27 CCCGAG C^[CT]CG[GA]G
BbsI 700 GTCTTC GAAGAC(2/6)
BbvCI 734 CCTCAGC CCTCAGC(-5/-2)
BcgI 505 CGACCCATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 481 CTAG C^TAG
BpmI 266 CTCCAG CTGGAG(16/14)
BpuAI 700 GTCTTC GAAGAC(2/6)
BsaI 767 GAGACC GGTCTC(1/5)
BsaWI 371 ACCGGA [AT]^CCGG[AT]
BsiEI 74 CGGCCG CG[GA][CT]^CG
BsiHKAI 30 GAGCTC G[AT]GC[AT]^C
BsmFI 542 GGGAC GGGAC(10/14)
BsoBI 27 CCCGAG C^[CT]CG[GA]G
BspHI 659 TCATGA T^CATGA
BsrBI 337 GAGCGG CCGCTC(-3/-3)
Bsu36I 634 CCTGAGG CC^T[ACGT]AGG
DpnII 297 GATC ^GATC
DraIII 106 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 74 CGGCCG C^GGCCG
EarI 310 GAAGAG CTCTTC(1/4)
EclXI 74 CGGCCG C^GGCCG
Eco52I 74 CGGCCG C^GGCCG
Eco81I 634 CCTGAGG CC^T[ACGT]AGG
HinfI 242 GAGTC G^A[ACGT]TC
Ksp632I 310 GAAGAG CTCTTC(1/4)
MaeI 481 CTAG C^TAG
MaeII 276 ACGT A^CGT
MaeIII 188 GTTAC ^GT[ACGT]AC
MboI 297 GATC ^GATC
MslI 654 CACACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 762 CAGCGG C[AC]G^C[GT]G
NdeII 297 GATC ^GATC
PleI 242 GAGTC GAGTC(4/5)
RcaI 659 TCATGA T^CATGA
SacI 30 GAGCTC GAGCT^C
Sau3AI 297 GATC ^GATC
SbfI 396 CCTGCAGG CCTGCA^GG
SexAI 107 ACCTGGT A^CC[AT]GGT
SfaNI 119 GCATC GCATC(5/9)
Sse8387I 396 CCTGCAGG CCTGCA^GG
TaqI 279 TCGA T^CGA
TthHB8I 279 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccII 63 CGCG CG^CG
334 CGCG
AlwNI 142 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
617 CAGGGCCTG
ApaI 178 GGGCCC GGGCC^C
251 GGGCCC
BanI 111 GGTGCC G^G[CT][GA]CC
562 GGCACC
BsgI 643 CTGCAC GTGCAG(16/14)
363 GTGCAG
BsrI 417 ACTGG ACTGG(1/-1)
557 CCAGT
BstUI 63 CGCG CG^CG
334 CGCG
EcoT14I 533 CCAAGG C^C[AT][AT]GG
786 CCTTGG
FauI 61 CCCGC CCCGC(4/6)
47 GCGGG
HaeII 317 AGCGCC [GA]GCGC^[CT]
476 AGCGCC
HphI 686 TCACC GGTGA(8/7)
704 TCACC
MvnI 63 CGCG CG^CG
334 CGCG
NlaIII 129 CATG CATG^
660 CATG
StuI 196 AGGCCT AGG^CCT
283 AGGCCT
StyI 533 CCAAGG C^C[AT][AT]GG
786 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI BanII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MboII MnlI MspI
MvaI MwoI NciI NlaIV PstI
RsaI Sau96I ScrFI SfcI TseI
TspRI