################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BbeI BbrPI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI DsaI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AocI 634 CCTGAGG CC^T[ACGT]AGG AspHI 30 GAGCTC G[AT]GC[AT]^C AvaI 27 CCCGAG C^[CT]CG[GA]G BbsI 700 GTCTTC GAAGAC(2/6) BbvCI 734 CCTCAGC CCTCAGC(-5/-2) BcgI 505 CGACCCATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 481 CTAG C^TAG BpmI 266 CTCCAG CTGGAG(16/14) BpuAI 700 GTCTTC GAAGAC(2/6) BsaI 767 GAGACC GGTCTC(1/5) BsaWI 371 ACCGGA [AT]^CCGG[AT] BsiEI 74 CGGCCG CG[GA][CT]^CG BsiHKAI 30 GAGCTC G[AT]GC[AT]^C BsmFI 542 GGGAC GGGAC(10/14) BsoBI 27 CCCGAG C^[CT]CG[GA]G BspHI 659 TCATGA T^CATGA BsrBI 337 GAGCGG CCGCTC(-3/-3) Bsu36I 634 CCTGAGG CC^T[ACGT]AGG DpnII 297 GATC ^GATC DraIII 106 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 74 CGGCCG C^GGCCG EarI 310 GAAGAG CTCTTC(1/4) EclXI 74 CGGCCG C^GGCCG Eco52I 74 CGGCCG C^GGCCG Eco81I 634 CCTGAGG CC^T[ACGT]AGG HinfI 242 GAGTC G^A[ACGT]TC Ksp632I 310 GAAGAG CTCTTC(1/4) MaeI 481 CTAG C^TAG MaeII 276 ACGT A^CGT MaeIII 188 GTTAC ^GT[ACGT]AC MboI 297 GATC ^GATC MslI 654 CACACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 762 CAGCGG C[AC]G^C[GT]G NdeII 297 GATC ^GATC PleI 242 GAGTC GAGTC(4/5) RcaI 659 TCATGA T^CATGA SacI 30 GAGCTC GAGCT^C Sau3AI 297 GATC ^GATC SbfI 396 CCTGCAGG CCTGCA^GG SexAI 107 ACCTGGT A^CC[AT]GGT SfaNI 119 GCATC GCATC(5/9) Sse8387I 396 CCTGCAGG CCTGCA^GG TaqI 279 TCGA T^CGA TthHB8I 279 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccII 63 CGCG CG^CG 334 CGCG AlwNI 142 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 617 CAGGGCCTG ApaI 178 GGGCCC GGGCC^C 251 GGGCCC BanI 111 GGTGCC G^G[CT][GA]CC 562 GGCACC BsgI 643 CTGCAC GTGCAG(16/14) 363 GTGCAG BsrI 417 ACTGG ACTGG(1/-1) 557 CCAGT BstUI 63 CGCG CG^CG 334 CGCG EcoT14I 533 CCAAGG C^C[AT][AT]GG 786 CCTTGG FauI 61 CCCGC CCCGC(4/6) 47 GCGGG HaeII 317 AGCGCC [GA]GCGC^[CT] 476 AGCGCC HphI 686 TCACC GGTGA(8/7) 704 TCACC MvnI 63 CGCG CG^CG 334 CGCG NlaIII 129 CATG CATG^ 660 CATG StuI 196 AGGCCT AGG^CCT 283 AGGCCT StyI 533 CCAAGG C^C[AT][AT]GG 786 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AfaI AluI BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MvaI MwoI NciI NlaIV PstI RsaI Sau96I ScrFI SfcI TseI TspRI