Restriction map for
pF1KB7561
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# NO CUT ENZYMES ( 203 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BbvI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssKI
BssSI Bst1107I BstBI BstEII BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FauI Fnu4HI FseI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII ItaI KasI KpnI KspI
MfeI MflI MluI MluNI MroI
MscI MslI MspI MunI MvaI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsaI RsrII SacI
SacII SalI Sau96I SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TseI TspRI
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 366 GTCTAC GT^[AC][GT]AC
BanI 311 GGTGCC G^G[CT][GA]CC
BbsI 127 GAAGAC GAAGAC(2/6)
BclI 120 TGATCA T^GATCA
BpuAI 127 GAAGAC GAAGAC(2/6)
BsaBI 166 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 499 GAGGAG GAGGAG(10/8)
BsiYI 217 CCTCAAACGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 217 CCTCAAACGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrI 516 CCAGT ACTGG(1/-1)
BstAPI 235 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 9 GGATG GGATG(2/0)
Cac8I 526 GCTGGC GC[ACGT]^[ACGT]GC
DpnII 121 GATC ^GATC
EarI 487 GAAGAG CTCTTC(1/4)
FbaI 120 TGATCA T^GATCA
FokI 9 GGATG GGATG(9/13)
HincII 548 GTCAAC GT[CT]^[GA]AC
HindII 548 GTCAAC GT[CT]^[GA]AC
HinfI 47 GAATC G^A[ACGT]TC
HphI 13 GGTGA GGTGA(8/7)
Ksp632I 487 GAAGAG CTCTTC(1/4)
MaeII 188 ACGT A^CGT
MamI 166 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 121 GATC ^GATC
MseI 532 TTAA T^TAA
MspA1I 524 CAGCTG C[AC]G^C[GT]G
NdeII 121 GATC ^GATC
PstI 53 CTGCAG CTGCA^G
PvuII 524 CAGCTG CAG^CTG
SapI 487 GAAGAGC GCTCTTC(1/4)
Sau3AI 121 GATC ^GATC
TfiI 47 GAATC G^A[AT]TC
Tru9I 532 TTAA T^TAA
XcmI 516 CCAGTTACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcsI 418 AAATTC [GA]^AATT[CT]
608 AAATTC
ApoI 418 AAATTC [GA]^AATT[CT]
608 AAATTC
Asp700I 250 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
346 GAAGATATTC
BfaI 43 CTAG C^TAG
194 CTAG
BspHI 93 TCATGA T^CATGA
123 TCATGA
DdeI 104 CTGAG C^T[ACGT]AG
481 CTAAG
MaeI 43 CTAG C^TAG
194 CTAG
NlaIV 311 GGTGCC GG[ACGT]^[ACGT]CC
393 GGAACC
RcaI 93 TCATGA T^CATGA
123 TCATGA
SfaNI 31 GATGC GCATC(5/9)
241 GATGC
SfcI 53 CTGCAG C^T[GA][CT]AG
470 CTACAG
Tsp45I 14 GTGAC ^GT[GC]AC
463 GTCAC
XmnI 250 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
346 GAAGATATTC
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
AluI MaeIII MboII MnlI MwoI
NlaIII Tsp509I