Restriction map for
pF1KB7562
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 127 GACGCC G[GA]^CG[CT]C
AlwNI 190 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 321 GGGCCC GGGCC^C
AspHI 715 GAGCAC G[AT]GC[AT]^C
BamHI 762 GGATCC G^GATCC
BbrPI 232 CACGTG CAC^GTG
BbsI 561 GTCTTC GAAGAC(2/6)
BbvCI 307 GCTGAGG CCTCAGC(-5/-2)
BglI 202 GCCGAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 561 GTCTTC GAAGAC(2/6)
BsaAI 232 CACGTG [CT]AC^GT[GA]
BsaHI 127 GACGCC G[GA]^CG[CT]C
BsiHKAI 715 GAGCAC G[AT]GC[AT]^C
BsmAI 150 GTCTC GTCTC(1/5)
BspHI 426 TCATGA T^CATGA
BspMI 723 ACCTGC ACCTGC(4/8)
BsrBI 403 CCGCTC CCGCTC(-3/-3)
BsrDI 17 GCAATG GCAATG(2/0)
BsrFI 101 GCCGGC [GA]^CCGG[CT]
BssSI 145 CACGAG CACGAG(-5/-1)
BstF5I 637 CATCC GGATG(2/0)
CfoI 243 GCGC GCG^C
Cfr10I 101 GCCGGC [GA]^CCGG[CT]
DsaI 254 CCATGG C^C[GA][CT]GG
EcoNI 174 CCTGCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 478 CCCGC CCCGC(4/6)
FokI 637 CATCC GGATG(9/13)
HaeII 242 AGCGCC [GA]GCGC^[CT]
HgaI 127 GACGC GACGC(5/10)
HhaI 243 GCGC GCG^C
Hin1I 127 GACGCC G[GA]^CG[CT]C
HinP1I 243 GCGC G^CGC
HphI 774 TCACC GGTGA(8/7)
MaeIII 389 GTTAC ^GT[ACGT]AC
NaeI 101 GCCGGC GCC^GGC
NcoI 254 CCATGG C^CATGG
NgoMIV 101 GCCGGC G^CCGGC
PaeR7I 625 CTCGAG C^TCGAG
PleI 148 GAGTC GAGTC(4/5)
PmaCI 232 CACGTG CAC^GTG
PmlI 232 CACGTG CAC^GTG
PpuMI 658 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 426 TCATGA T^CATGA
SfaNI 636 GCATC GCATC(5/9)
SmaI 378 CCCGGG CCC^GGG
TaqI 626 TCGA T^CGA
TfiI 430 GATTC G^A[AT]TC
TthHB8I 626 TCGA T^CGA
XcmI 644 CCAGAGGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 625 CTCGAG C^TCGAG
XmaCI 378 CCCGGG C^CCGGG
XmaI 378 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaI 378 CCCGGG C^[CT]CG[GA]G
625 CTCGAG
BlpI 272 GCTCAGC GC^T[ACGT]AGC
397 GCTCAGC
Bpu1102I 272 GCTCAGC GC^T[ACGT]AGC
397 GCTCAGC
BseRI 442 CTCCTC GAGGAG(10/8)
801 CTCCTC
BsgI 133 CTGCAC GTGCAG(16/14)
229 CTGCAC
BsoBI 378 CCCGGG C^[CT]CG[GA]G
625 CTCGAG
BstYI 185 AGATCC [GA]^GATC[CT]
762 GGATCC
CelII 272 GCTCAGC GC^T[ACGT]AGC
397 GCTCAGC
DpnII 186 GATC ^GATC
763 GATC
DraII 321 GGGCCCT [GA]G^G[ACGT]CC[CT]
658 GGGTCCT
EcoO109I 321 GGGCCCT [GA]G^G[ACGT]CC[CT]
658 GGGTCCT
HinfI 148 GAGTC G^A[ACGT]TC
430 GATTC
MaeII 233 ACGT A^CGT
699 ACGT
MboI 186 GATC ^GATC
763 GATC
MflI 185 AGATCC [GA]^GATC[CT]
762 GGATCC
MslI 4 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
232 CACGTGCATG
NdeII 186 GATC ^GATC
763 GATC
Sau3AI 186 GATC ^GATC
763 GATC
SbfI 195 CCTGCAGG CCTGCA^GG
552 CCTGCAGG
SmlI 625 CTCGAG C^T[CT][GA]AG
809 CTCAAG
Sse8387I 195 CCTGCAGG CCTGCA^GG
552 CCTGCAGG
XhoII 185 AGATCC [GA]^GATC[CT]
762 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DdeI
EcoRII EcoT14I Fnu4HI HaeIII HapII
HpaII ItaI MboII MnlI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PstI PvuII Sau96I ScrFI
SfcI StyI TseI TspRI