Restriction map for
pF1KB7565
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BfaI BfrI
BglI BglII BlnI BpmI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I TspRI Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcsI 118 GAATTC [GA]^AATT[CT]
AfaI 45 GTAC GT^AC
ApaI 519 GGGCCC GGGCC^C
ApoI 118 GAATTC [GA]^AATT[CT]
AspHI 76 GAGCAC G[AT]GC[AT]^C
AspI 150 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 270 GGACC G^G[AT]CC
BanII 519 GGGCCC G[GA]GC[CT]^C
BbsI 147 GAAGAC GAAGAC(2/6)
BciVI 369 GGATAC GTATCC(6/5)
BlpI 445 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 445 GCTAAGC GC^T[ACGT]AGC
BpuAI 147 GAAGAC GAAGAC(2/6)
BsaI 11 GAGACC GGTCTC(1/5)
BsgI 58 GTGCAG GTGCAG(16/14)
BsiHKAI 76 GAGCAC G[AT]GC[AT]^C
BsiWI 44 CGTACG C^GTACG
BsiYI 238 CCTCCCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 238 CCTCCCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 11 GAGAC GTCTC(1/5)
BspMI 64 ACCTGC ACCTGC(4/8)
BstAPI 481 GCATATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 612 GGATG GGATG(2/0)
CelII 445 GCTAAGC GC^T[ACGT]AGC
DpnII 336 GATC ^GATC
DrdI 97 GACTCTTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 25 CCGCGG C^C[GA][CT]GG
EcoNI 238 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 118 GAATTC G^AATTC
EcoT14I 491 CCAAGG C^C[AT][AT]GG
FauI 27 GCGGG CCCGC(4/6)
FokI 612 GGATG GGATG(9/13)
HinfI 97 GACTC G^A[ACGT]TC
KspI 25 CCGCGG CCGC^GG
MaeII 233 ACGT A^CGT
MaeIII 197 GTGAC ^GT[ACGT]AC
MboI 336 GATC ^GATC
MspA1I 25 CCGCGG C[AC]G^C[GT]G
MwoI 481 GCATATCGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 428 CATATG CA^TATG
NdeII 336 GATC ^GATC
NlaIII 153 CATG CATG^
PflFI 150 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 97 GACTC GAGTC(4/5)
PpuMI 269 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 45 GTAC GT^AC
SacII 25 CCGCGG CCGC^GG
SapI 73 GAAGAGC GCTCTTC(1/4)
Sau3AI 336 GATC ^GATC
StyI 491 CCAAGG C^C[AT][AT]GG
TaqI 172 TCGA T^CGA
Tsp45I 197 GTGAC ^GT[GC]AC
Tth111I 150 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 172 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccII 26 CGCG CG^CG
114 CGCG
BbvI 442 GCAGC GCAGC(8/12)
478 GCTGC
BcnI 69 CCGGG CC^[GC]GG
93 CCCGG
BmyI 76 GAGCAC G[AGT]GC[ACT]^C
519 GGGCCC
Bsp1286I 76 GAGCAC G[AGT]GC[ACT]^C
519 GGGCCC
BstNI 352 CCTGG CC^[AT]GG
523 CCTGG
BstUI 26 CGCG CG^CG
114 CGCG
Cac8I 205 GCTCGC GC[ACGT]^[ACGT]GC
527 GCAGGC
DdeI 401 CTGAG C^T[ACGT]AG
446 CTAAG
EcoRII 352 CCTGG ^CC[AT]GG
523 CCTGG
HapII 69 CCGG C^CGG
94 CCGG
HpaII 69 CCGG C^CGG
94 CCGG
HphI 247 GGTGA GGTGA(8/7)
388 GGTGA
MspI 69 CCGG C^CGG
94 CCGG
MvaI 352 CCTGG CC^[AT]GG
523 CCTGG
MvnI 26 CGCG CG^CG
114 CGCG
NciI 69 CCGGG CC^[GC]GG
93 CCCGG
NlaIV 270 GGACCC GG[ACGT]^[ACGT]CC
519 GGGCCC
TseI 442 GCAGC G^C[AT]GC
478 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI AluI BsaJI BseRI BssKI
Cfr13I DraII EarI EcoO109I Fnu4HI
HaeIII ItaI Ksp632I MboII MnlI
Sau96I ScrFI Tsp509I