##################################
# NO CUT ENZYMES ( 182 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiWI BsmFI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HaeIII HapII HgaI
Hin1I HpaII KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Aor51HI 614 AGCGCT AGC^GCT
AvaI 365 CTCGGG C^[CT]CG[GA]G
BciVI 582 GGATAC GTATCC(6/5)
BfaI 374 CTAG C^TAG
BglII 96 AGATCT A^GATCT
BsaI 76 GAGACC GGTCTC(1/5)
BsaJI 80 CCAAGG C^C[ACGT][ACGT]GG
BseRI 142 GAGGAG GAGGAG(10/8)
BsiEI 5 CGACCG CG[GA][CT]^CG
BsiYI 397 CCAACAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 397 CCAACAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 10 GAGACG CGTCTC(1/5)
BsmI 305 GAATGC GAATGC(1/-1)
BsoBI 365 CTCGGG C^[CT]CG[GA]G
BssKI 114 CCAGG ^CC[ACGT]GG
BssSI 65 CTCGTG CACGAG(-5/-1)
BstNI 114 CCAGG CC^[AT]GG
BstYI 96 AGATCT [GA]^GATC[CT]
Cac8I 36 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 615 GCGC GCG^C
DdeI 299 CTGAG C^T[ACGT]AG
DpnII 97 GATC ^GATC
Eco47III 614 AGCGCT AGC^GCT
EcoRII 114 CCAGG ^CC[AT]GG
EcoT14I 80 CCAAGG C^C[AT][AT]GG
HaeII 614 AGCGCT [GA]GCGC^[CT]
HhaI 615 GCGC GCG^C
HinP1I 615 GCGC G^CGC
HincII 433 GTTAAC GT[CT]^[GA]AC
HindII 433 GTTAAC GT[CT]^[GA]AC
HindIII 34 AAGCTT A^AGCTT
HpaI 433 GTTAAC GTT^AAC
HphI 270 GGTGA GGTGA(8/7)
MaeI 374 CTAG C^TAG
MaeIII 550 GTCAC ^GT[ACGT]AC
MboI 97 GATC ^GATC
MflI 96 AGATCT [GA]^GATC[CT]
MseI 434 TTAA T^TAA
MspA1I 560 CAGCTG C[AC]G^C[GT]G
MvaI 114 CCAGG CC^[AT]GG
NdeII 97 GATC ^GATC
NlaIII 133 CATG CATG^
NlaIV 571 GGAACC GG[ACGT]^[ACGT]CC
NspI 132 ACATGC [GA]CATG^[CT]
PleI 110 GACTC GAGTC(4/5)
PvuII 560 CAGCTG CAG^CTG
Sau3AI 97 GATC ^GATC
ScrFI 114 CCAGG CC^[ACGT]GG
StyI 80 CCAAGG C^C[AT][AT]GG
TfiI 326 GATTC G^A[AT]TC
Tru9I 434 TTAA T^TAA
Tsp45I 550 GTCAC ^GT[GC]AC
Tsp509I 276 AATT ^AATT
XhoII 96 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 5 enzymes) #
###################################
AfaI 163 GTAC GT^AC
334 GTAC
BpmI 112 CTCCAG CTGGAG(16/14)
492 CTCCAG
BsrI 165 ACTGG ACTGG(1/-1)
568 ACTGG
HinfI 110 GACTC G^A[ACGT]TC
326 GATTC
RsaI 163 GTAC GT^AC
334 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI AspHI BanII BbvI BmyI
BsiHKAI BsmAI Bsp1286I EarI Fnu4HI
ItaI Ksp632I MboII MnlI MwoI
SacI TseI