################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiWI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeIII HapII HgaI Hin1I HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Aor51HI 614 AGCGCT AGC^GCT AvaI 365 CTCGGG C^[CT]CG[GA]G BciVI 582 GGATAC GTATCC(6/5) BfaI 374 CTAG C^TAG BglII 96 AGATCT A^GATCT BsaI 76 GAGACC GGTCTC(1/5) BsaJI 80 CCAAGG C^C[ACGT][ACGT]GG BseRI 142 GAGGAG GAGGAG(10/8) BsiEI 5 CGACCG CG[GA][CT]^CG BsiYI 397 CCAACAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 397 CCAACAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 10 GAGACG CGTCTC(1/5) BsmI 305 GAATGC GAATGC(1/-1) BsoBI 365 CTCGGG C^[CT]CG[GA]G BssKI 114 CCAGG ^CC[ACGT]GG BssSI 65 CTCGTG CACGAG(-5/-1) BstNI 114 CCAGG CC^[AT]GG BstYI 96 AGATCT [GA]^GATC[CT] Cac8I 36 GCTTGC GC[ACGT]^[ACGT]GC CfoI 615 GCGC GCG^C DdeI 299 CTGAG C^T[ACGT]AG DpnII 97 GATC ^GATC Eco47III 614 AGCGCT AGC^GCT EcoRII 114 CCAGG ^CC[AT]GG EcoT14I 80 CCAAGG C^C[AT][AT]GG HaeII 614 AGCGCT [GA]GCGC^[CT] HhaI 615 GCGC GCG^C HinP1I 615 GCGC G^CGC HincII 433 GTTAAC GT[CT]^[GA]AC HindII 433 GTTAAC GT[CT]^[GA]AC HindIII 34 AAGCTT A^AGCTT HpaI 433 GTTAAC GTT^AAC HphI 270 GGTGA GGTGA(8/7) MaeI 374 CTAG C^TAG MaeIII 550 GTCAC ^GT[ACGT]AC MboI 97 GATC ^GATC MflI 96 AGATCT [GA]^GATC[CT] MseI 434 TTAA T^TAA MspA1I 560 CAGCTG C[AC]G^C[GT]G MvaI 114 CCAGG CC^[AT]GG NdeII 97 GATC ^GATC NlaIII 133 CATG CATG^ NlaIV 571 GGAACC GG[ACGT]^[ACGT]CC NspI 132 ACATGC [GA]CATG^[CT] PleI 110 GACTC GAGTC(4/5) PvuII 560 CAGCTG CAG^CTG Sau3AI 97 GATC ^GATC ScrFI 114 CCAGG CC^[ACGT]GG StyI 80 CCAAGG C^C[AT][AT]GG TfiI 326 GATTC G^A[AT]TC Tru9I 434 TTAA T^TAA Tsp45I 550 GTCAC ^GT[GC]AC Tsp509I 276 AATT ^AATT XhoII 96 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 5 enzymes) # ################################### AfaI 163 GTAC GT^AC 334 GTAC BpmI 112 CTCCAG CTGGAG(16/14) 492 CTCCAG BsrI 165 ACTGG ACTGG(1/-1) 568 ACTGG HinfI 110 GACTC G^A[ACGT]TC 326 GATTC RsaI 163 GTAC GT^AC 334 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AluI AspHI BanII BbvI BmyI BsiHKAI BsmAI Bsp1286I EarI Fnu4HI ItaI Ksp632I MboII MnlI MwoI SacI TseI