Restriction map for
pF1KB7587
##################################
# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HgaI Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AfaI 381 GTAC GT^AC
Alw44I 524 GTGCAC G^TGCAC
ApaLI 524 GTGCAC G^TGCAC
AviII 365 TGCGCA TGC^GCA
BanI 471 GGTGCC G^G[CT][GA]CC
BcnI 444 CCGGG CC^[GC]GG
BglI 298 GCCTTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 405 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 405 GCTCAGC GC^T[ACGT]AGC
BseRI 424 GAGGAG GAGGAG(10/8)
BsgI 592 CTGCAC GTGCAG(16/14)
BsmAI 13 GAGAC GTCTC(1/5)
BsmFI 166 GGGAC GGGAC(10/14)
BsmI 481 GAATGC GAATGC(1/-1)
BsrBI 106 GAGCGG CCGCTC(-3/-3)
BsrFI 242 ACCGGC [GA]^CCGG[CT]
BstBI 88 TTCGAA TT^CGAA
BstYI 359 AGATCC [GA]^GATC[CT]
CelII 405 GCTCAGC GC^T[ACGT]AGC
Cfr10I 242 ACCGGC [GA]^CCGG[CT]
EaeI 254 CGGCCA [CT]^GGCC[GA]
EarI 312 GAAGAG CTCTTC(1/4)
EcoNI 414 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 350 CCAAGG C^C[AT][AT]GG
FspI 365 TGCGCA TGC^GCA
Ksp632I 312 GAAGAG CTCTTC(1/4)
MaeIII 431 GTGAC ^GT[ACGT]AC
MflI 359 AGATCC [GA]^GATC[CT]
MslI 621 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 340 CAGCGG C[AC]G^C[GT]G
NciI 444 CCGGG CC^[GC]GG
NlaIII 663 CATG CATG^
NspV 88 TTCGAA TT^CGAA
PpuMI 166 GGGACCC [GA]G^G[AT]CC[CT]
PstI 504 CTGCAG CTGCA^G
RsaI 381 GTAC GT^AC
SacI 115 GAGCTC GAGCT^C
SfuI 88 TTCGAA TT^CGAA
StuI 296 AGGCCT AGG^CCT
StyI 350 CCAAGG C^C[AT][AT]GG
Tsp45I 431 GTGAC ^GT[GC]AC
XhoII 359 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 360 GATCC GGATC(4/5)
552 GGATC
ApaI 142 GGGCCC GGGCC^C
457 GGGCCC
AvaI 130 CCCGAG C^[CT]CG[GA]G
172 CCCGAG
AvaII 167 GGACC G^G[AT]CC
240 GGACC
BbvI 485 GCAGC GCAGC(8/12)
345 GCTGC
BsoBI 130 CCCGAG C^[CT]CG[GA]G
172 CCCGAG
BspMI 65 ACCTGC ACCTGC(4/8)
590 ACCTGC
BstF5I 17 CATCC GGATG(2/0)
375 CATCC
DpnII 360 GATC ^GATC
553 GATC
DsaI 199 CCGTGG C^C[GA][CT]GG
236 CCGTGG
FokI 17 CATCC GGATG(9/13)
375 CATCC
HapII 243 CCGG C^CGG
444 CCGG
HpaII 243 CCGG C^CGG
444 CCGG
MboI 360 GATC ^GATC
553 GATC
MboII 312 GAAGA GAAGA(8/7)
631 GAAGA
MspI 243 CCGG C^CGG
444 CCGG
NdeII 360 GATC ^GATC
553 GATC
Sau3AI 360 GATC ^GATC
553 GATC
SfaNI 580 GATGC GCATC(5/9)
620 GCATC
SfcI 504 CTGCAG C^T[GA][CT]AG
575 CTACAG
SmlI 148 CTTGAG C^T[CT][GA]AG
280 CTCAAG
TseI 345 GCTGC G^C[AT]GC
485 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AlwNI AspHI BanII
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DraII
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HhaI HinP1I HphI ItaI
MnlI MvaI MwoI NlaIV Sau96I
ScrFI TaqI TspRI TthHB8I