Restriction map for
pF1KB7592
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# NO CUT ENZYMES ( 182 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BbvI BcgI BciVI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BsrI BssHII BssKI
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRII
EcoRV EcoT22I FauI Fnu4HI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HpaI HpaII HphI ItaI
KasI KpnI Ksp632I KspI MaeI
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvaI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI ScrFI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TseI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AclI 71 AACGTT AA^CGTT
AfaI 426 GTAC GT^AC
AlwNI 649 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 640 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 44 GAGCTC G[AT]GC[AT]^C
BamHI 3 GGATCC G^GATCC
BanII 44 GAGCTC G[GA]GC[CT]^C
BclI 165 TGATCA T^GATCA
BmyI 44 GAGCTC G[AGT]GC[ACT]^C
BpmI 833 CTGGAG CTGGAG(16/14)
BsaBI 277 GATACGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 680 CCATGG C^C[ACGT][ACGT]GG
BsiHKAI 44 GAGCTC G[AT]GC[AT]^C
BsiYI 449 CCCTACTTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 449 CCCTACTTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 44 GAGCTC G[AGT]GC[ACT]^C
BsrDI 173 GCAATG GCAATG(2/0)
BstYI 3 GGATCC [GA]^GATC[CT]
Cac8I 783 GCCTGC GC[ACGT]^[ACGT]GC
DraII 446 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 680 CCATGG C^C[GA][CT]GG
EcoO109I 446 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 494 GAATTC G^AATTC
EcoT14I 680 CCATGG C^C[AT][AT]GG
FbaI 165 TGATCA T^GATCA
HgaI 803 GACGC GACGC(5/10)
HincII 373 GTCAAC GT[CT]^[GA]AC
HindII 373 GTCAAC GT[CT]^[GA]AC
HindIII 194 AAGCTT A^AGCTT
MaeIII 667 GTCAC ^GT[ACGT]AC
MamI 277 GATACGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3 GGATCC [GA]^GATC[CT]
MslI 169 CATAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 680 CCATGG C^CATGG
Psp1406I 71 AACGTT AA^CGTT
RsaI 426 GTAC GT^AC
SacI 44 GAGCTC GAGCT^C
SmlI 337 CTCAAG C^T[CT][GA]AG
StyI 680 CCATGG C^C[AT][AT]GG
Tsp45I 667 GTCAC ^GT[GC]AC
XhoII 3 GGATCC [GA]^GATC[CT]
XmnI 640 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AvaII 558 GGACC G^G[AT]CC
745 GGACC
BbsI 255 GAAGAC GAAGAC(2/6)
505 GAAGAC
BpuAI 255 GAAGAC GAAGAC(2/6)
505 GAAGAC
BsmFI 557 GGGAC GGGAC(10/14)
581 GTCCC
HaeIII 447 GGCC GG^CC
759 GGCC
MaeII 72 ACGT A^CGT
431 ACGT
MseI 185 TTAA T^TAA
809 TTAA
MwoI 471 GCATGACCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
541 GCTGTCAAGGC
SfcI 59 CTGTAG C^T[GA][CT]AG
671 CTGTAG
TfiI 676 GATTC G^A[AT]TC
739 GAATC
Tru9I 185 TTAA T^TAA
809 TTAA
TspRI 521 CACTG CA[GC]TG[ACGT][ACGT]^
669 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AcsI AluI AlwI ApoI BseRI
BstF5I Cfr13I DdeI DpnII FokI
HinfI MboI MboII MnlI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
SfaNI Tsp509I