################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsaI RsrII SacII SalI SapI SbfI ScaI ScrFI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AseI 43 ATTAAT AT^TAAT AsnI 43 ATTAAT AT^TAAT BanII 214 GAGCTC G[GA]GC[CT]^C BclI 582 TGATCA T^GATCA BfaI 553 CTAG C^TAG BglII 858 AGATCT A^GATCT BpmI 706 CTGGAG CTGGAG(16/14) BseRI 709 GAGGAG GAGGAG(10/8) BsgI 760 CTGCAC GTGCAG(16/14) BsiYI 447 CCTAAACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 447 CCTAAACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BstF5I 537 GGATG GGATG(2/0) BstYI 858 AGATCT [GA]^GATC[CT] Cac8I 125 GCAGGC GC[ACGT]^[ACGT]GC EarI 475 GAAGAG CTCTTC(1/4) EcoNI 447 CCTAAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 582 TGATCA T^GATCA FokI 537 GGATG GGATG(9/13) HphI 146 TCACC GGTGA(8/7) Ksp632I 475 GAAGAG CTCTTC(1/4) MaeI 553 CTAG C^TAG MflI 858 AGATCT [GA]^GATC[CT] MslI 28 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 523 GCTACAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PleI 83 GACTC GAGTC(4/5) PshBI 43 ATTAAT AT^TAAT SacI 214 GAGCTC GAGCT^C TaqI 813 TCGA T^CGA TthHB8I 813 TCGA T^CGA XhoII 858 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflII 286 CTTAAG C^TTAAG 621 CTTAAG AspHI 22 GAGCAC G[AT]GC[AT]^C 214 GAGCTC BbsI 504 GAAGAC GAAGAC(2/6) 669 GAAGAC BfrI 286 CTTAAG C^TTAAG 621 CTTAAG BmyI 22 GAGCAC G[AGT]GC[ACT]^C 214 GAGCTC BpuAI 504 GAAGAC GAAGAC(2/6) 669 GAAGAC BsiHKAI 22 GAGCAC G[AT]GC[AT]^C 214 GAGCTC BsmAI 81 GAGAC GTCTC(1/5) 160 GTCTC Bsp1286I 22 GAGCAC G[AGT]GC[ACT]^C 214 GAGCTC Cfr13I 12 GGGCC G^G[ACGT]CC 612 GGCCC HaeIII 13 GGCC GG^CC 612 GGCC MspA1I 256 CAGCTG C[AC]G^C[GT]G 757 CAGCTG NlaIII 765 CATG CATG^ 819 CATG PvuII 256 CAGCTG CAG^CTG 757 CAGCTG Sau96I 12 GGGCC G^G[ACGT]CC 612 GGCCC SfcI 92 CTGTAG C^T[GA][CT]AG 292 CTACAG Tsp45I 397 GTCAC ^GT[GC]AC 702 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AcsI AluI ApoI BbvI BsrI DdeI DpnII Fnu4HI HinfI ItaI MaeIII MboI MboII MnlI MseI NdeII Sau3AI SmlI TfiI Tru9I TseI Tsp509I TspRI